A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth

Abstract Objectives This study aimed to assess the microtensile bond strength of a chlorhexidine-containing dentin bonding agent compared with a standard adhesive, and to investigate its clinical success in Class II cavities in primary teeth, with a 12-month follow-up. Materials and methods The stud...

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Main Authors: Hülya Altıntop, Hasibe Elif Kuru, Fahinur Ertuğrul, Murat Türkün, Ebru Küçükyılmaz
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Oral Health
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Online Access:https://doi.org/10.1186/s12903-025-05960-0
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author Hülya Altıntop
Hasibe Elif Kuru
Fahinur Ertuğrul
Murat Türkün
Ebru Küçükyılmaz
author_facet Hülya Altıntop
Hasibe Elif Kuru
Fahinur Ertuğrul
Murat Türkün
Ebru Küçükyılmaz
author_sort Hülya Altıntop
collection DOAJ
description Abstract Objectives This study aimed to assess the microtensile bond strength of a chlorhexidine-containing dentin bonding agent compared with a standard adhesive, and to investigate its clinical success in Class II cavities in primary teeth, with a 12-month follow-up. Materials and methods The study consisted of two parts: a prospective, single-blind, split-mouth randomized controlled clinical trial and an in vitro laboratory evaluation. Ethical approval were obtained for the both parts of the study. Forty pediatric patients (aged 5–9 years) with primary molars requiring Class II restorations were included in the clinical trial, where bulk-fill restorations bonded with a chlorhexidine-containing adhesive and a standard adhesive were evaluated based on FDI criteria at 3-month intervals for 12 months. he in vitro study examined the microtensile bond strength (µTBS) of immediate and thermally aged specimens prepared with bulk-fill composite materials and adhesives with or without chlorhexidine.The data were analyzed using Mann Whitney U test for in-vivo, ANOVA test followed by Tukey’s post hoc and Chi-square test for in-vitro study (p = 0.05). Results Clinically, both adhesives showed similar success rates (%100) across all FDI evaluation parameters after 12 months (p > 0.05). The chlorhexidine-containing adhesive demonstrated significantly higher microtensile bond strength than the standard adhesive in both immediate and aged samples (p < 0.05). Conclusions The chlorhexidine-containing adhesive showed promising clinical success and improved bond strength compared to the standard adhesive. Longer follow-ups are needed to confirm its long-term durability.“Incorporating chlorhexidine simplifies restorative procedures without compromising performance. Clinical Relevance Chlorhexidine-containing adhesives may improve bond durability and procedural efficiency in pediatric dentistry, offering a practical and effective alternative for restoring primary molars. Trial registration Invivo part of the study was registered in a public trial registry, www.clinicaltrials.gov (#NCT06257108). Registration Date 2nd. May 2024 (Retrospectively registered).
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issn 1472-6831
language English
publishDate 2025-04-01
publisher BMC
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series BMC Oral Health
spelling doaj-art-4307d1d9fefd48b096aabd9a4ec102792025-08-20T03:18:39ZengBMCBMC Oral Health1472-68312025-04-0125111510.1186/s12903-025-05960-0A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teethHülya Altıntop0Hasibe Elif Kuru1Fahinur Ertuğrul2Murat Türkün3Ebru Küçükyılmaz4Faculty of Dentistry, Department of Pediatric Dentistry, İzmir Katip Çelebi UniversityFaculty of Dentistry, Department of Pediatric Dentistry, Uşak UniversityFaculty of Dentistry, Department of Pediatric Dentistry, Ege UniversityFaculty of Dentistry, Department of Restorative Dentistry, Ege UniversityFaculty of Dentistry, Department of Pediatric Dentistry, İzmir Katip Çelebi UniversityAbstract Objectives This study aimed to assess the microtensile bond strength of a chlorhexidine-containing dentin bonding agent compared with a standard adhesive, and to investigate its clinical success in Class II cavities in primary teeth, with a 12-month follow-up. Materials and methods The study consisted of two parts: a prospective, single-blind, split-mouth randomized controlled clinical trial and an in vitro laboratory evaluation. Ethical approval were obtained for the both parts of the study. Forty pediatric patients (aged 5–9 years) with primary molars requiring Class II restorations were included in the clinical trial, where bulk-fill restorations bonded with a chlorhexidine-containing adhesive and a standard adhesive were evaluated based on FDI criteria at 3-month intervals for 12 months. he in vitro study examined the microtensile bond strength (µTBS) of immediate and thermally aged specimens prepared with bulk-fill composite materials and adhesives with or without chlorhexidine.The data were analyzed using Mann Whitney U test for in-vivo, ANOVA test followed by Tukey’s post hoc and Chi-square test for in-vitro study (p = 0.05). Results Clinically, both adhesives showed similar success rates (%100) across all FDI evaluation parameters after 12 months (p > 0.05). The chlorhexidine-containing adhesive demonstrated significantly higher microtensile bond strength than the standard adhesive in both immediate and aged samples (p < 0.05). Conclusions The chlorhexidine-containing adhesive showed promising clinical success and improved bond strength compared to the standard adhesive. Longer follow-ups are needed to confirm its long-term durability.“Incorporating chlorhexidine simplifies restorative procedures without compromising performance. Clinical Relevance Chlorhexidine-containing adhesives may improve bond durability and procedural efficiency in pediatric dentistry, offering a practical and effective alternative for restoring primary molars. Trial registration Invivo part of the study was registered in a public trial registry, www.clinicaltrials.gov (#NCT06257108). Registration Date 2nd. May 2024 (Retrospectively registered).https://doi.org/10.1186/s12903-025-05960-0ChlorhexidineDentin bondingMicrotensile bond strengthPrimary molarsClass II restorationClinical study
spellingShingle Hülya Altıntop
Hasibe Elif Kuru
Fahinur Ertuğrul
Murat Türkün
Ebru Küçükyılmaz
A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
BMC Oral Health
Chlorhexidine
Dentin bonding
Microtensile bond strength
Primary molars
Class II restoration
Clinical study
title A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
title_full A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
title_fullStr A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
title_full_unstemmed A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
title_short A prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine-containing dentin bonding agent and a bulk fill composite material in primary teeth
title_sort prospective randomized clinical trial and an in vitro evaluation of the microtensile bond strength of a chlorhexidine containing dentin bonding agent and a bulk fill composite material in primary teeth
topic Chlorhexidine
Dentin bonding
Microtensile bond strength
Primary molars
Class II restoration
Clinical study
url https://doi.org/10.1186/s12903-025-05960-0
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