Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors

This study aimed to investigate the impact of incorporating mature compost on the harmlessness and maturity level of composting from swine manure and carcasses from industrialized pig farms in continuously running large-scale silo reactor systems. The potential human or animal bacterial pathogens an...

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Main Authors: Ziwei Jiao, Liping Zhang, Ake Zhang, Ruoqi Li, Kui Zhang, Zhen Wu, Zitong Kang, Yuquan Wei, Longli Zhang, Yue Wang, Xiong Shi, Ji Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494332/full
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author Ziwei Jiao
Liping Zhang
Ake Zhang
Ake Zhang
Ruoqi Li
Kui Zhang
Zhen Wu
Zitong Kang
Yuquan Wei
Longli Zhang
Yue Wang
Xiong Shi
Ji Li
author_facet Ziwei Jiao
Liping Zhang
Ake Zhang
Ake Zhang
Ruoqi Li
Kui Zhang
Zhen Wu
Zitong Kang
Yuquan Wei
Longli Zhang
Yue Wang
Xiong Shi
Ji Li
author_sort Ziwei Jiao
collection DOAJ
description This study aimed to investigate the impact of incorporating mature compost on the harmlessness and maturity level of composting from swine manure and carcasses from industrialized pig farms in continuously running large-scale silo reactor systems. The potential human or animal bacterial pathogens and core bacterial community in composting were analyzed by high-throughput sequencing of 16S rRNA gene amplicons. The results showed that the addition of mature compost in the GD group significantly increased the temperature of all depths, the accumulated temperature of compost, and the germination index (75.43%) compared to that in the HN group without mature compost. High-throughput sequencing revealed that the dominated genera in GD were Ureibacillus, Lactobacillus, Corynebacterium, Staphylococcus, and Jeotgalicoccus, and the addition of mature compost could significantly increase the relative abundance of Ureibacillus (16.82%) that was associated with the biodegradation of organics. A total of 421 potential bacterial pathogens were detected, and the dominated genera of pathogens were Streptococcus, Staphylococcus, and Anaerococcus. The potential pathogen in the GD group with mature compost was reduced from 7.16 to 0.77%, which was significantly lower than that (2.97%) in the HN group. Together, these findings revealed that mature compost addition in large-scale reactor composting could accelerate the harmless and humification process, providing an effective and environmentally friendly scheme to deal with the main organic wastes in intensive pig farms.
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spelling doaj-art-42fd93e203bb4c8c9c304e33aa0bb9952025-08-20T02:14:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14943321494332Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactorsZiwei Jiao0Liping Zhang1Ake Zhang2Ake Zhang3Ruoqi Li4Kui Zhang5Zhen Wu6Zitong Kang7Yuquan Wei8Longli Zhang9Yue Wang10Xiong Shi11Ji Li12College of Biological Science and Technology, Yili Normal University, Yining, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaFuyang Agricultural Science Academy, Fuyang, Anhui, ChinaCollege of Agricultural Science and Engineering, Hohai University, Nanjing, Jiangsu, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaBeijing VOTO Biotech Co., Ltd., Beijing, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaYangtze Eco-Environment Engineering Research Center, China Three Gorges Corporation, Wuhan, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, ChinaThis study aimed to investigate the impact of incorporating mature compost on the harmlessness and maturity level of composting from swine manure and carcasses from industrialized pig farms in continuously running large-scale silo reactor systems. The potential human or animal bacterial pathogens and core bacterial community in composting were analyzed by high-throughput sequencing of 16S rRNA gene amplicons. The results showed that the addition of mature compost in the GD group significantly increased the temperature of all depths, the accumulated temperature of compost, and the germination index (75.43%) compared to that in the HN group without mature compost. High-throughput sequencing revealed that the dominated genera in GD were Ureibacillus, Lactobacillus, Corynebacterium, Staphylococcus, and Jeotgalicoccus, and the addition of mature compost could significantly increase the relative abundance of Ureibacillus (16.82%) that was associated with the biodegradation of organics. A total of 421 potential bacterial pathogens were detected, and the dominated genera of pathogens were Streptococcus, Staphylococcus, and Anaerococcus. The potential pathogen in the GD group with mature compost was reduced from 7.16 to 0.77%, which was significantly lower than that (2.97%) in the HN group. Together, these findings revealed that mature compost addition in large-scale reactor composting could accelerate the harmless and humification process, providing an effective and environmentally friendly scheme to deal with the main organic wastes in intensive pig farms.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494332/fullfull-scale compostingsilo reactorswine manurebacterial communitypotential pathogenmature compost
spellingShingle Ziwei Jiao
Liping Zhang
Ake Zhang
Ake Zhang
Ruoqi Li
Kui Zhang
Zhen Wu
Zitong Kang
Yuquan Wei
Longli Zhang
Yue Wang
Xiong Shi
Ji Li
Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
Frontiers in Microbiology
full-scale composting
silo reactor
swine manure
bacterial community
potential pathogen
mature compost
title Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
title_full Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
title_fullStr Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
title_full_unstemmed Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
title_short Mature compost enhanced the harmlessness level in co-composting swine manure and carcasses in large-scale silo reactors
title_sort mature compost enhanced the harmlessness level in co composting swine manure and carcasses in large scale silo reactors
topic full-scale composting
silo reactor
swine manure
bacterial community
potential pathogen
mature compost
url https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494332/full
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