Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers
Increasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggests that miRNA might be exploitable as signatures of exposure to specific infectious agents. In order to identify potential early miRNA biomarkers of bacterial infections, human per...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/6489383 |
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| author | Regina Z. Cer J. Enrique Herrera-Galeano Kenneth G. Frey Kevin L. Schully Truong V. Luu John Pesce Vishwesh P. Mokashi Andrea M. Keane-Myers Kimberly A. Bishop-Lilly |
| author_facet | Regina Z. Cer J. Enrique Herrera-Galeano Kenneth G. Frey Kevin L. Schully Truong V. Luu John Pesce Vishwesh P. Mokashi Andrea M. Keane-Myers Kimberly A. Bishop-Lilly |
| author_sort | Regina Z. Cer |
| collection | DOAJ |
| description | Increasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggests that miRNA might be exploitable as signatures of exposure to specific infectious agents. In order to identify potential early miRNA biomarkers of bacterial infections, human peripheral blood mononuclear cells (hPBMCs) were exposed to two select agents, Burkholderia pseudomallei K96243 and Francisella tularensis SHU S4, as well as to the nonpathogenic control Escherichia coli DH5α. RNA samples were harvested at three early time points, 30, 60, and 120 minutes postexposure, then sequenced. RNAseq analyses identified 87 miRNAs to be differentially expressed (DE) in a linear fashion. Of these, 31 miRNAs were tested using the miScript miRNA qPCR assay. Through RNAseq identification and qPCR validation, we identified differentially expressed miRNA species that may be involved in the early response to bacterial infections. Based upon its upregulation at early time points postexposure in two different individuals, hsa-mir-30c-5p is a miRNA species that could be studied further as a potential biomarker for exposure to these gram-negative intracellular pathogens. Gene ontology functional analyses demonstrated that programmed cell death is the first ranking biological process associated with miRNAs that are upregulated in F. tularensis-exposed hPBMCs. |
| format | Article |
| id | doaj-art-42f8fb111f8c45c784fdd6750ea3efbc |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-42f8fb111f8c45c784fdd6750ea3efbc2025-08-20T02:37:58ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/64893836489383Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential BiomarkersRegina Z. Cer0J. Enrique Herrera-Galeano1Kenneth G. Frey2Kevin L. Schully3Truong V. Luu4John Pesce5Vishwesh P. Mokashi6Andrea M. Keane-Myers7Kimberly A. Bishop-Lilly8Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAGenomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center, Frederick, MD, USAIncreasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggests that miRNA might be exploitable as signatures of exposure to specific infectious agents. In order to identify potential early miRNA biomarkers of bacterial infections, human peripheral blood mononuclear cells (hPBMCs) were exposed to two select agents, Burkholderia pseudomallei K96243 and Francisella tularensis SHU S4, as well as to the nonpathogenic control Escherichia coli DH5α. RNA samples were harvested at three early time points, 30, 60, and 120 minutes postexposure, then sequenced. RNAseq analyses identified 87 miRNAs to be differentially expressed (DE) in a linear fashion. Of these, 31 miRNAs were tested using the miScript miRNA qPCR assay. Through RNAseq identification and qPCR validation, we identified differentially expressed miRNA species that may be involved in the early response to bacterial infections. Based upon its upregulation at early time points postexposure in two different individuals, hsa-mir-30c-5p is a miRNA species that could be studied further as a potential biomarker for exposure to these gram-negative intracellular pathogens. Gene ontology functional analyses demonstrated that programmed cell death is the first ranking biological process associated with miRNAs that are upregulated in F. tularensis-exposed hPBMCs.http://dx.doi.org/10.1155/2017/6489383 |
| spellingShingle | Regina Z. Cer J. Enrique Herrera-Galeano Kenneth G. Frey Kevin L. Schully Truong V. Luu John Pesce Vishwesh P. Mokashi Andrea M. Keane-Myers Kimberly A. Bishop-Lilly Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers International Journal of Genomics |
| title | Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers |
| title_full | Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers |
| title_fullStr | Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers |
| title_full_unstemmed | Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers |
| title_short | Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers |
| title_sort | differential microrna analyses of burkholderia pseudomallei and francisella tularensis exposed hpbmcs reveal potential biomarkers |
| url | http://dx.doi.org/10.1155/2017/6489383 |
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