Full-thickness cervix reconstruction via collagen scaffolds in rabbits
Congenital deficiency or extensive cervical injury leads to female genital tract obstruction and cervical laxity, resulting in infertility or miscarriage. Current clinical approaches could be conducted to restore the continuity of cervix. However, full-thickness and large-scale cervical regeneration...
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KeAi Communications Co., Ltd.
2025-05-01
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| Series: | Bioactive Materials |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X25000155 |
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| author | Yaqian Li Weijie Tian Jianbin Guo Yang Ye Qianqian Gao Yiwei Zhang Xiaoyue Zhao Jianwu Dai Lan Zhu |
| author_facet | Yaqian Li Weijie Tian Jianbin Guo Yang Ye Qianqian Gao Yiwei Zhang Xiaoyue Zhao Jianwu Dai Lan Zhu |
| author_sort | Yaqian Li |
| collection | DOAJ |
| description | Congenital deficiency or extensive cervical injury leads to female genital tract obstruction and cervical laxity, resulting in infertility or miscarriage. Current clinical approaches could be conducted to restore the continuity of cervix. However, full-thickness and large-scale cervical regeneration with complete structural and functional restoration have not been reported. In this study, we fabricated a double-layered collagen membrane (CM) scaffold based on decellularized extracellular matrix. Each layer was adapted to support the growth of epithelial cells and stromal cells, respectively. Further, the thickness and folded “sandwich” structure were tailored to match the cervical structure. When transplanted into rabbit full-thickness and total ectocervix excision models, only CM scaffold groups enabled the regeneration of neo-ectocervix tissue, including epithelium, stroma and muscular layers after 3 and 6 months. The neo-ectocervix regenerated by CM scaffolds exhibited significantly higher expression of secretory glands and estrogen receptors, more secretion of neutral and acidic mucins, showing functional maturity of regenerated epithelium. Notably, CM scaffolds supported the regeneration of stroma and muscular layers. The mechanical strength of neo ectocervix was comparable to that of normal ectocervix. CM scaffolds demonstrate good biocompatibility, support different cell growth, and enhance superior regeneration of epithelium, stromal and muscular tissue, confirming its construction capacity as new strategies for addressing cervical deficiency and damage. |
| format | Article |
| id | doaj-art-42f79b86174a40c986e6b88bc29d419e |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-42f79b86174a40c986e6b88bc29d419e2025-01-23T05:27:23ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-05-0147170180Full-thickness cervix reconstruction via collagen scaffolds in rabbitsYaqian Li0Weijie Tian1Jianbin Guo2Yang Ye3Qianqian Gao4Yiwei Zhang5Xiaoyue Zhao6Jianwu Dai7Lan Zhu8Institute of Clinical Medicine, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China; Department of Gynecology, Guizhou Provincial People's Hospital, Medical College of Guizhou University, Guiyang, 550002, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China; Department of Obstetrics and Gynecology, Shandong Provincial Health Commission Key Laboratory of Prevention and Treatment for Major Gynecological Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Ji'nan, Shandong, 250021, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China; Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Ji'nan, Shandong, 250012, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, ChinaDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100080, China; Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China; Corresponding author. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, State Key Laboratory of Common Mechanism Research for Major Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China; Corresponding author.Congenital deficiency or extensive cervical injury leads to female genital tract obstruction and cervical laxity, resulting in infertility or miscarriage. Current clinical approaches could be conducted to restore the continuity of cervix. However, full-thickness and large-scale cervical regeneration with complete structural and functional restoration have not been reported. In this study, we fabricated a double-layered collagen membrane (CM) scaffold based on decellularized extracellular matrix. Each layer was adapted to support the growth of epithelial cells and stromal cells, respectively. Further, the thickness and folded “sandwich” structure were tailored to match the cervical structure. When transplanted into rabbit full-thickness and total ectocervix excision models, only CM scaffold groups enabled the regeneration of neo-ectocervix tissue, including epithelium, stroma and muscular layers after 3 and 6 months. The neo-ectocervix regenerated by CM scaffolds exhibited significantly higher expression of secretory glands and estrogen receptors, more secretion of neutral and acidic mucins, showing functional maturity of regenerated epithelium. Notably, CM scaffolds supported the regeneration of stroma and muscular layers. The mechanical strength of neo ectocervix was comparable to that of normal ectocervix. CM scaffolds demonstrate good biocompatibility, support different cell growth, and enhance superior regeneration of epithelium, stromal and muscular tissue, confirming its construction capacity as new strategies for addressing cervical deficiency and damage.http://www.sciencedirect.com/science/article/pii/S2452199X25000155 |
| spellingShingle | Yaqian Li Weijie Tian Jianbin Guo Yang Ye Qianqian Gao Yiwei Zhang Xiaoyue Zhao Jianwu Dai Lan Zhu Full-thickness cervix reconstruction via collagen scaffolds in rabbits Bioactive Materials |
| title | Full-thickness cervix reconstruction via collagen scaffolds in rabbits |
| title_full | Full-thickness cervix reconstruction via collagen scaffolds in rabbits |
| title_fullStr | Full-thickness cervix reconstruction via collagen scaffolds in rabbits |
| title_full_unstemmed | Full-thickness cervix reconstruction via collagen scaffolds in rabbits |
| title_short | Full-thickness cervix reconstruction via collagen scaffolds in rabbits |
| title_sort | full thickness cervix reconstruction via collagen scaffolds in rabbits |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X25000155 |
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