Chromosome-level genome assembly of the Rhizoctonia solani

Abstract Rhizoctonia solani is a ubiquitously distributed soil-borne fungal pathogen that causes serious diseases in many plants worldwide. It attracts significant research attention due to its considerable economic importance in agricultural production. However, the limited availability of genome i...

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Main Authors: Haoxue Xia, Tianbo Liu, Yaru He, Qitang Guo, Hanxiang Wu, Guangfei Tang
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05351-8
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author Haoxue Xia
Tianbo Liu
Yaru He
Qitang Guo
Hanxiang Wu
Guangfei Tang
author_facet Haoxue Xia
Tianbo Liu
Yaru He
Qitang Guo
Hanxiang Wu
Guangfei Tang
author_sort Haoxue Xia
collection DOAJ
description Abstract Rhizoctonia solani is a ubiquitously distributed soil-borne fungal pathogen that causes serious diseases in many plants worldwide. It attracts significant research attention due to its considerable economic importance in agricultural production. However, the limited availability of genome information has further impeded the development of new molecular-targeted control technologies. By utilizing Illumina short-read, PacBio HiFi long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, we present a comprehensive and continuous chromosome-level assembly for R. solani. The final genome size is 40,801,261 bp, consisting of 23 contigs with a N50 of 2,529,230 bp. Hi-C data aids in anchoring the assembly onto 16 chromosomes. Additionally, the genome contains 16.17% (6,597,897 bp) repeat elements, including 10,698 protein-coding genes and 232 non-coding RNAs. The high-quality genome of R. solani not only provides valuable genomic information for further comprehending the fungal pathobiology and evolution, but also contributes to the development of scientific control strategies for disease prevention and control in agriculture.
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spelling doaj-art-42f486ef67134b458f15c70fced440dc2025-08-20T02:36:50ZengNature PortfolioScientific Data2052-44632025-06-011211810.1038/s41597-025-05351-8Chromosome-level genome assembly of the Rhizoctonia solaniHaoxue Xia0Tianbo Liu1Yaru He2Qitang Guo3Hanxiang Wu4Guangfei Tang5State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesChina Tobacco Corporation Hunan CompanyState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesDatian Branch of Sanming Tobacco Company, SanmingState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAbstract Rhizoctonia solani is a ubiquitously distributed soil-borne fungal pathogen that causes serious diseases in many plants worldwide. It attracts significant research attention due to its considerable economic importance in agricultural production. However, the limited availability of genome information has further impeded the development of new molecular-targeted control technologies. By utilizing Illumina short-read, PacBio HiFi long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, we present a comprehensive and continuous chromosome-level assembly for R. solani. The final genome size is 40,801,261 bp, consisting of 23 contigs with a N50 of 2,529,230 bp. Hi-C data aids in anchoring the assembly onto 16 chromosomes. Additionally, the genome contains 16.17% (6,597,897 bp) repeat elements, including 10,698 protein-coding genes and 232 non-coding RNAs. The high-quality genome of R. solani not only provides valuable genomic information for further comprehending the fungal pathobiology and evolution, but also contributes to the development of scientific control strategies for disease prevention and control in agriculture.https://doi.org/10.1038/s41597-025-05351-8
spellingShingle Haoxue Xia
Tianbo Liu
Yaru He
Qitang Guo
Hanxiang Wu
Guangfei Tang
Chromosome-level genome assembly of the Rhizoctonia solani
Scientific Data
title Chromosome-level genome assembly of the Rhizoctonia solani
title_full Chromosome-level genome assembly of the Rhizoctonia solani
title_fullStr Chromosome-level genome assembly of the Rhizoctonia solani
title_full_unstemmed Chromosome-level genome assembly of the Rhizoctonia solani
title_short Chromosome-level genome assembly of the Rhizoctonia solani
title_sort chromosome level genome assembly of the rhizoctonia solani
url https://doi.org/10.1038/s41597-025-05351-8
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AT yaruhe chromosomelevelgenomeassemblyoftherhizoctoniasolani
AT qitangguo chromosomelevelgenomeassemblyoftherhizoctoniasolani
AT hanxiangwu chromosomelevelgenomeassemblyoftherhizoctoniasolani
AT guangfeitang chromosomelevelgenomeassemblyoftherhizoctoniasolani