Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction

Re-introduction of captive endangering or extinct animals to their native habitats holds profound ecological significance. But evaluating the feralization progress of re-introduced “wild” animals requires concise and quantitative indicator(s) to integrate traditional assessments on self-sustainabili...

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Main Authors: Min Zhou, Muyang Wang, Weikang Yang, Yuanming Zhang, Shen Yu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Global Ecology and Conservation
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Online Access:http://www.sciencedirect.com/science/article/pii/S2351989424004451
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author Min Zhou
Muyang Wang
Weikang Yang
Yuanming Zhang
Shen Yu
author_facet Min Zhou
Muyang Wang
Weikang Yang
Yuanming Zhang
Shen Yu
author_sort Min Zhou
collection DOAJ
description Re-introduction of captive endangering or extinct animals to their native habitats holds profound ecological significance. But evaluating the feralization progress of re-introduced “wild” animals requires concise and quantitative indicator(s) to integrate traditional assessments on self-sustainability and reproduction. A fecal ARGs’ profiling was conducted for Przewalski’s horses, an extinct flagship herbivore, in Xinjiang, China from the Breeding Center (captive), Tianshan Wildlife Zoo (semi-captive), and Kalamaili Nature Reserve (re-introduction) with Mongolian wild asses serving as a reference in the shared Kalamaili range. Over 60 ARGs were quantified across 6 samples at each site for Przewalski’s horses while 46 ARGs for Mongolian wild asses. The re-introduced Przewalski’s horses had significant losses of fecal ARGs in sharing number and relative abundance in comparison to the captive ones. The re-introduced Przewalski’s horses had complex internal connections among the quantified fecal ARGs in expression potential (average relative abundance per quantified ARGs by antimicrobial category) and even more significant external connections with Mongolian wild asses than with captive horse. Overall, the re-introduction of Przewalski’s horses achieved notable and progressive success in feralization. Tetracyclines resistance genes (Tetra-RGs) alone, with full quantification frequency, could quantitatively characterized this achievement. Ratio of Tetra-RGs to aminoglycosides resistant genes (Amino-RGs) would differentiate the feralization progress with orders of magnitude, for the re-introduced Przewalski’s horses at 279 and the captive one at 11.7, against Mongolian wild asses at 0.86 in this study. The two ARGs-based indicators might be applicable to quantify feralization progress in re-introduction of other captive endangering or extinct animals with validations.
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spelling doaj-art-42d1b324659e46fc9c3f9741eb82a9a02025-08-20T02:49:08ZengElsevierGlobal Ecology and Conservation2351-98942024-12-0156e0324110.1016/j.gecco.2024.e03241Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introductionMin Zhou0Muyang Wang1Weikang Yang2Yuanming Zhang3Shen Yu4Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Corresponding authors.State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; Corresponding authors.Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Corresponding authors.Re-introduction of captive endangering or extinct animals to their native habitats holds profound ecological significance. But evaluating the feralization progress of re-introduced “wild” animals requires concise and quantitative indicator(s) to integrate traditional assessments on self-sustainability and reproduction. A fecal ARGs’ profiling was conducted for Przewalski’s horses, an extinct flagship herbivore, in Xinjiang, China from the Breeding Center (captive), Tianshan Wildlife Zoo (semi-captive), and Kalamaili Nature Reserve (re-introduction) with Mongolian wild asses serving as a reference in the shared Kalamaili range. Over 60 ARGs were quantified across 6 samples at each site for Przewalski’s horses while 46 ARGs for Mongolian wild asses. The re-introduced Przewalski’s horses had significant losses of fecal ARGs in sharing number and relative abundance in comparison to the captive ones. The re-introduced Przewalski’s horses had complex internal connections among the quantified fecal ARGs in expression potential (average relative abundance per quantified ARGs by antimicrobial category) and even more significant external connections with Mongolian wild asses than with captive horse. Overall, the re-introduction of Przewalski’s horses achieved notable and progressive success in feralization. Tetracyclines resistance genes (Tetra-RGs) alone, with full quantification frequency, could quantitatively characterized this achievement. Ratio of Tetra-RGs to aminoglycosides resistant genes (Amino-RGs) would differentiate the feralization progress with orders of magnitude, for the re-introduced Przewalski’s horses at 279 and the captive one at 11.7, against Mongolian wild asses at 0.86 in this study. The two ARGs-based indicators might be applicable to quantify feralization progress in re-introduction of other captive endangering or extinct animals with validations.http://www.sciencedirect.com/science/article/pii/S2351989424004451ARGs profilingFaecesFeralization indicatorPrzewalski’s horses (Equus ferus)Re-introduction
spellingShingle Min Zhou
Muyang Wang
Weikang Yang
Yuanming Zhang
Shen Yu
Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
Global Ecology and Conservation
ARGs profiling
Faeces
Feralization indicator
Przewalski’s horses (Equus ferus)
Re-introduction
title Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
title_full Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
title_fullStr Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
title_full_unstemmed Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
title_short Fecal ARGs characterized progressive feralization of Przewalski's horses via re-introduction
title_sort fecal args characterized progressive feralization of przewalski s horses via re introduction
topic ARGs profiling
Faeces
Feralization indicator
Przewalski’s horses (Equus ferus)
Re-introduction
url http://www.sciencedirect.com/science/article/pii/S2351989424004451
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