Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development

Summary: During animal development, cells communicate to ensure tissue-wide synchronization of differentiation. While several mechanisms contributing to cell coordination have been described, whether additional mechanisms are at play should cells locally desynchronize remains unknown. Here, we inves...

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Main Authors: Maleaume Soulard, Diego Andrés Contreras, Bruno Monier, Thomas Mangeat, Vanessa Dougados, Jennifer Zanet, Francis Corson, Vincent Hakim, François Payre, Anne Pélissier-Monier
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589004225013963
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author Maleaume Soulard
Diego Andrés Contreras
Bruno Monier
Thomas Mangeat
Vanessa Dougados
Jennifer Zanet
Francis Corson
Vincent Hakim
François Payre
Anne Pélissier-Monier
author_facet Maleaume Soulard
Diego Andrés Contreras
Bruno Monier
Thomas Mangeat
Vanessa Dougados
Jennifer Zanet
Francis Corson
Vincent Hakim
François Payre
Anne Pélissier-Monier
author_sort Maleaume Soulard
collection DOAJ
description Summary: During animal development, cells communicate to ensure tissue-wide synchronization of differentiation. While several mechanisms contributing to cell coordination have been described, whether additional mechanisms are at play should cells locally desynchronize remains unknown. Here, we investigate the responses to experimentally induced desynchronized cells during Drosophila epidermis development. We report that cells that behave as if they were “too young” or “too old”, collectively referred to as heterochronous cells, sort out from their normally timed neighbors. Cell sorting is associated with alterations of junctions, cytoskeleton, and cell mechanics. Moreover, local heterochrony ultimately leads to cell elimination. Importantly, we find that some cells naturally undergo either premature or delayed differentiation during development and are similarly eliminated from the tissue. These results show that local imbalance in differentiation timing affects both cell interactions and mechanics, leading to cell sorting, and elimination as a way to correct local heterochrony and safeguard the synchrony of epithelium differentiation.
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institution Kabale University
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spelling doaj-art-42c9373d09c74135a535c2995e3dfd542025-08-20T03:32:58ZengElsevieriScience2589-00422025-08-0128811313510.1016/j.isci.2025.113135Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue developmentMaleaume Soulard0Diego Andrés Contreras1Bruno Monier2Thomas Mangeat3Vanessa Dougados4Jennifer Zanet5Francis Corson6Vincent Hakim7François Payre8Anne Pélissier-Monier9Molecular Cellular and Developmental Biology (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31000 Toulouse, FranceLaboratoire de Physique de l’Ecole Normale Supérieure, CNRS, Ecole Normale Supérieure, PSL University, Sorbonne Université, Université Paris Cité, Paris, FranceMolecular Cellular and Developmental Biology (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31000 Toulouse, FranceLITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, FranceLITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, FranceMolecular Cellular and Developmental Biology (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31000 Toulouse, FranceLaboratoire de Physique de l’Ecole Normale Supérieure, CNRS, Ecole Normale Supérieure, PSL University, Sorbonne Université, Université Paris Cité, Paris, FranceLaboratoire de Physique de l’Ecole Normale Supérieure, CNRS, Ecole Normale Supérieure, PSL University, Sorbonne Université, Université Paris Cité, Paris, FranceMolecular Cellular and Developmental Biology (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31000 Toulouse, FranceMolecular Cellular and Developmental Biology (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31000 Toulouse, France; Corresponding authorSummary: During animal development, cells communicate to ensure tissue-wide synchronization of differentiation. While several mechanisms contributing to cell coordination have been described, whether additional mechanisms are at play should cells locally desynchronize remains unknown. Here, we investigate the responses to experimentally induced desynchronized cells during Drosophila epidermis development. We report that cells that behave as if they were “too young” or “too old”, collectively referred to as heterochronous cells, sort out from their normally timed neighbors. Cell sorting is associated with alterations of junctions, cytoskeleton, and cell mechanics. Moreover, local heterochrony ultimately leads to cell elimination. Importantly, we find that some cells naturally undergo either premature or delayed differentiation during development and are similarly eliminated from the tissue. These results show that local imbalance in differentiation timing affects both cell interactions and mechanics, leading to cell sorting, and elimination as a way to correct local heterochrony and safeguard the synchrony of epithelium differentiation.http://www.sciencedirect.com/science/article/pii/S2589004225013963Cell biologyDevelopmental biology
spellingShingle Maleaume Soulard
Diego Andrés Contreras
Bruno Monier
Thomas Mangeat
Vanessa Dougados
Jennifer Zanet
Francis Corson
Vincent Hakim
François Payre
Anne Pélissier-Monier
Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
iScience
Cell biology
Developmental biology
title Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
title_full Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
title_fullStr Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
title_full_unstemmed Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
title_short Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
title_sort elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
topic Cell biology
Developmental biology
url http://www.sciencedirect.com/science/article/pii/S2589004225013963
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