A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants
Pseudomonas syringae infects a wide variety of crops. The mangotoxin-generating operon (mgo) is conserved across many P. syringae strains and is responsible for producing an extracellular chemical signal, leudiazen. Disruption of the mgoA gene in P. syringae pv. syringae (Pss) UMAF0158 alleviated to...
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The American Phytopathological Society
2024-11-01
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| Series: | Molecular Plant-Microbe Interactions |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-24-0069-R |
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| author | Qiang Guo Caitlin N. Vitro Drake M. Crawford Bo Li |
| author_facet | Qiang Guo Caitlin N. Vitro Drake M. Crawford Bo Li |
| author_sort | Qiang Guo |
| collection | DOAJ |
| description | Pseudomonas syringae infects a wide variety of crops. The mangotoxin-generating operon (mgo) is conserved across many P. syringae strains and is responsible for producing an extracellular chemical signal, leudiazen. Disruption of the mgoA gene in P. syringae pv. syringae (Pss) UMAF0158 alleviated tomato chlorosis caused by this bacterium. We showed that deletion of the entire mgo reduced Pss UMAF0158 population in tomato leaflets. Leudiazen restored the signaling activity of the deletion mutant at a concentration as low as 10 nM. Both the diazeniumdiolate and isobutyl groups of leudiazen are critical for this potent signaling activity. Transcriptional analysis showed that mgo and leudiazen induce the expression of mangotoxin biosynthetic operon as well as an uncharacterized gene cluster, RS17235–RS17245. We found that this cluster enhances the survival of Pss UMAF0158 in planta and is widely distributed in P. syringae strains. Our results demonstrate that mgo plays prominent roles in the virulence and growth of P. syringae. The mgo and mgo-like signaling systems in different bacteria likely regulate diverse microbe-host interactions. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| format | Article |
| id | doaj-art-42c31cb51cfe45ef85fdd85917c3cd6c |
| institution | Kabale University |
| issn | 0894-0282 1943-7706 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj-art-42c31cb51cfe45ef85fdd85917c3cd6c2024-12-04T16:16:57ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062024-11-01371177678310.1094/MPMI-06-24-0069-RA Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in PlantsQiang Guo0Caitlin N. Vitro1Drake M. Crawford2Bo Li3MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaDepartment of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, U.S.A.Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, U.S.A.Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, U.S.A.Pseudomonas syringae infects a wide variety of crops. The mangotoxin-generating operon (mgo) is conserved across many P. syringae strains and is responsible for producing an extracellular chemical signal, leudiazen. Disruption of the mgoA gene in P. syringae pv. syringae (Pss) UMAF0158 alleviated tomato chlorosis caused by this bacterium. We showed that deletion of the entire mgo reduced Pss UMAF0158 population in tomato leaflets. Leudiazen restored the signaling activity of the deletion mutant at a concentration as low as 10 nM. Both the diazeniumdiolate and isobutyl groups of leudiazen are critical for this potent signaling activity. Transcriptional analysis showed that mgo and leudiazen induce the expression of mangotoxin biosynthetic operon as well as an uncharacterized gene cluster, RS17235–RS17245. We found that this cluster enhances the survival of Pss UMAF0158 in planta and is widely distributed in P. syringae strains. Our results demonstrate that mgo plays prominent roles in the virulence and growth of P. syringae. The mgo and mgo-like signaling systems in different bacteria likely regulate diverse microbe-host interactions. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-24-0069-Rbacteria-plant interactionextracellular chemical signalingquorum sensingRNA sequencing analysisstructure-activity relationship |
| spellingShingle | Qiang Guo Caitlin N. Vitro Drake M. Crawford Bo Li A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants Molecular Plant-Microbe Interactions bacteria-plant interaction extracellular chemical signaling quorum sensing RNA sequencing analysis structure-activity relationship |
| title | A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants |
| title_full | A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants |
| title_fullStr | A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants |
| title_full_unstemmed | A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants |
| title_short | A Diazeniumdiolate Signal in Pseudomonas syringae Upregulates Virulence Factors and Promotes Survival in Plants |
| title_sort | diazeniumdiolate signal in pseudomonas syringae upregulates virulence factors and promotes survival in plants |
| topic | bacteria-plant interaction extracellular chemical signaling quorum sensing RNA sequencing analysis structure-activity relationship |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-24-0069-R |
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