Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line
Objective: Gastric cancer is a complex disease. Natural antioxidant sources without toxic side effects are suitable for the treatment and management of diseases such as cancer. Satureja khuzistanica (S. khuzistanica) is an endemic plant in southern Iran. Despite numerous articles published about thi...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-04-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/evaluating-the-effect-of-chloroform-and-water-extract-of-lessigreatersatureja-khuzistanicalessigreater-on-the-expression-of-bcl-2-and-bax-in-gastric-cancer-cell-line/doi/gmj.2022.3547 |
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| author | Bagher Biglak Asghar Tanomand Leyla Açik Farzad Arjomandi Rad Gholamreza Shahsavari Saman Kazemi Hossein Soltanzadeh |
| author_facet | Bagher Biglak Asghar Tanomand Leyla Açik Farzad Arjomandi Rad Gholamreza Shahsavari Saman Kazemi Hossein Soltanzadeh |
| author_sort | Bagher Biglak |
| collection | DOAJ |
| description | Objective: Gastric cancer is a complex disease. Natural antioxidant sources without toxic side effects are suitable for the treatment and management of diseases such as cancer. Satureja khuzistanica (S. khuzistanica) is an endemic plant in southern Iran. Despite numerous articles published about this plant, a limited number of studies have been conducted on its anticancer effects. Therefore, this study was conducted to evaluate the cytotoxic and apoptotic effects of this plant on the gastric cancer cell line.
Methods: The antimicrobial activities of chloroform and water extracts were screened against pathogenic bacteria [Helicobacter pylori (H. pylori)]. The cytotoxicity effect of the extracts was evaluated using an microculture tetrazolium assay on gastric cancer cell lines (AGS and KATO III) and normal gingival fibroblasts. The expression of Bax and Bcl-2 was evaluated using real-time polymerase chain reaction. Finally, all data were analyzed using REST software.
Results: Both extracts showed good inhibitory activity against H. pylori. In addition, extracts showed cytotoxic activity against gastric cancer cell lines. Treatment of S. khuzistanica cells increased and decreased the expression of Bax and Bcl-2 apoptotic genes, respectively (p<0.05). In addition, in HGF cells, there were no cytotoxic effects or apoptosis induction triggered by the extracts.
Conclusion: The chloroform extract had more cytotoxic and apoptotic effects than the water extract. The extracts could have anticancer and apoptotic effects on the gastric cancer cell line. |
| format | Article |
| id | doaj-art-42bee54b20794310bbc2329f591e9fdb |
| institution | Kabale University |
| issn | 2147-2092 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-42bee54b20794310bbc2329f591e9fdb2025-01-17T07:01:48ZengGalenos Publishing HouseGazi Medical Journal2147-20922024-04-0135211512010.12996/gmj.2022.3547Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell LineBagher Biglak0https://orcid.org/0000-0002-2102-6846Asghar Tanomand1https://orcid.org/0000-0002-4488-8414Leyla Açik2https://orcid.org/0000-0002-3672-8429Farzad Arjomandi Rad3https://orcid.org/0000-0002-4864-5831Gholamreza Shahsavari4https://orcid.org/0000-0003-2840-5192Saman Kazemi5https://orcid.org/0000-0001-7789-1399Hossein Soltanzadeh6https://orcid.org/0000-0003-4416-7593Department of Biology, Islamic Azad University Faculty of Basic Sciences, Bonab Branch, IranMedicinal Plants Research Center, Maragheh University of Medical Sciences, Maragheh, IranDepartment of Biology, Gazi University Faculty of Health Sciences, Ankara, TürkiyeDepartment of Chemistry, Islamic Azad University Faculty of Medicine, Bonab Branch, IranDepartment of Clinical Biochemistry, Lorestan University of Medical Sciences Faculty of Medicine, Khorramabad, IranDepartment of Biology, Islamic Azad University Faculty of Basic Sciences, Bonab Branch, IranDepartment of Biology, Islamic Azad University Faculty of Basic Sciences, Bonab Branch, IranObjective: Gastric cancer is a complex disease. Natural antioxidant sources without toxic side effects are suitable for the treatment and management of diseases such as cancer. Satureja khuzistanica (S. khuzistanica) is an endemic plant in southern Iran. Despite numerous articles published about this plant, a limited number of studies have been conducted on its anticancer effects. Therefore, this study was conducted to evaluate the cytotoxic and apoptotic effects of this plant on the gastric cancer cell line. Methods: The antimicrobial activities of chloroform and water extracts were screened against pathogenic bacteria [Helicobacter pylori (H. pylori)]. The cytotoxicity effect of the extracts was evaluated using an microculture tetrazolium assay on gastric cancer cell lines (AGS and KATO III) and normal gingival fibroblasts. The expression of Bax and Bcl-2 was evaluated using real-time polymerase chain reaction. Finally, all data were analyzed using REST software. Results: Both extracts showed good inhibitory activity against H. pylori. In addition, extracts showed cytotoxic activity against gastric cancer cell lines. Treatment of S. khuzistanica cells increased and decreased the expression of Bax and Bcl-2 apoptotic genes, respectively (p<0.05). In addition, in HGF cells, there were no cytotoxic effects or apoptosis induction triggered by the extracts. Conclusion: The chloroform extract had more cytotoxic and apoptotic effects than the water extract. The extracts could have anticancer and apoptotic effects on the gastric cancer cell line.https://gazimedj.com/articles/evaluating-the-effect-of-chloroform-and-water-extract-of-lessigreatersatureja-khuzistanicalessigreater-on-the-expression-of-bcl-2-and-bax-in-gastric-cancer-cell-line/doi/gmj.2022.3547ags and kato-iii cell lineapoptotic effectgastric cancersatureja khuzestanica |
| spellingShingle | Bagher Biglak Asghar Tanomand Leyla Açik Farzad Arjomandi Rad Gholamreza Shahsavari Saman Kazemi Hossein Soltanzadeh Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line Gazi Medical Journal ags and kato-iii cell line apoptotic effect gastric cancer satureja khuzestanica |
| title | Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line |
| title_full | Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line |
| title_fullStr | Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line |
| title_full_unstemmed | Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line |
| title_short | Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line |
| title_sort | evaluating the effect of chloroform and water extract of satureja khuzistanica on the expression of bcl 2 and bax in gastric cancer cell line |
| topic | ags and kato-iii cell line apoptotic effect gastric cancer satureja khuzestanica |
| url | https://gazimedj.com/articles/evaluating-the-effect-of-chloroform-and-water-extract-of-lessigreatersatureja-khuzistanicalessigreater-on-the-expression-of-bcl-2-and-bax-in-gastric-cancer-cell-line/doi/gmj.2022.3547 |
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