Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale
To better understand the indole-3-acetic acid (IAA) response pathway and explore the specific functions of Aux/IAA gene family in the growth of Chinese kale and in response to salt stress, forty-one Aux/IAA genes were collected and analyzed by bioinformatics methods from Chinese kale genome. Phyloge...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.10.221 |
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| Summary: | To better understand the indole-3-acetic acid (IAA) response pathway and explore the specific functions of Aux/IAA gene family in the growth of Chinese kale and in response to salt stress, forty-one Aux/IAA genes were collected and analyzed by bioinformatics methods from Chinese kale genome. Phylogenetic analysis showed that the Aux/IAA genes of Chinese kale were distributed on nine chromosomes and the most genes were distributed on chromosome C01 and C05 including six members, respectively. The number of the amino acid residues of proteins encoded by Aux/IAA genes ranged from 128 to 427, and most of them were hydrophilic proteins, and only two were hydrophobic proteins. The subcellular localization analysis indicated that the Aux/IAA family members were mostly located in cytoplasm, nucleus and chloroplast. The results of real-time quantitative polymerase chain reaction (qRT-PCR) showed that several Aux/IAA genes of Chinese kale responded to IAA, abscisic acid (ABA) and brassinosteroids (BR) treatments, which suggested that the Aux/IAA gene family was probably involved in plant hormone signal transduction process. Additionally, under salt stress treatment, the expression of BoIAA19-1 and BoIAA2-1 genes in Chinese kale were significantly up-regulated during the early treatment stage, indicating that Aux/IAA genes may also play roles in response to diverse environmental stresses. The results of this research lay theoretical foundations for further exploring the function of the Aux/IAA gene family in the growth and resistance balance of Chinese kale. |
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| ISSN: | 1008-9209 2097-5155 |