Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review

The depletion of superoxide catalyzed by human manganese superoxide dismutase (MnSOD) was observed spectrophotometrically by measuring the absorbance of superoxide at 250-280 nm following pulse radiolysis and it showed a biphasic pattern. Tryptophan 161 is a highly conserved residue that forms a hyd...

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Main Author: Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
Format: Article
Language:English
Published: Hammer Head Production Limited 2016-07-01
Series:Sokoto Journal of Medical Laboratory Science
Subjects:
Online Access:https://sokjmls.com.ng/index.php/SJMLS/article/view/369
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author Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
author_facet Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
author_sort Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
collection DOAJ
description The depletion of superoxide catalyzed by human manganese superoxide dismutase (MnSOD) was observed spectrophotometrically by measuring the absorbance of superoxide at 250-280 nm following pulse radiolysis and it showed a biphasic pattern. Tryptophan 161 is a highly conserved residue that forms a hydrophobic side of the active site cavity of manganese superoxide dismutase (MnSOD), with its indole ring adjacent to and about 5 Å from the manganese. Trp 161 promotes the dissociation of product peroxide, perhaps in part through its effect on the orientation of Tyr 34.
format Article
id doaj-art-42ab22f1007d4903ae49e113e143f9db
institution DOAJ
issn 2536-7153
language English
publishDate 2016-07-01
publisher Hammer Head Production Limited
record_format Article
series Sokoto Journal of Medical Laboratory Science
spelling doaj-art-42ab22f1007d4903ae49e113e143f9db2025-08-20T02:52:05ZengHammer Head Production LimitedSokoto Journal of Medical Laboratory Science2536-71532016-07-0111369Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A ReviewAlhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.The depletion of superoxide catalyzed by human manganese superoxide dismutase (MnSOD) was observed spectrophotometrically by measuring the absorbance of superoxide at 250-280 nm following pulse radiolysis and it showed a biphasic pattern. Tryptophan 161 is a highly conserved residue that forms a hydrophobic side of the active site cavity of manganese superoxide dismutase (MnSOD), with its indole ring adjacent to and about 5 Å from the manganese. Trp 161 promotes the dissociation of product peroxide, perhaps in part through its effect on the orientation of Tyr 34.https://sokjmls.com.ng/index.php/SJMLS/article/view/369manganese, pulse radiolysis, superoxide dismutase, tryptophan
spellingShingle Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
Sokoto Journal of Medical Laboratory Science
manganese, pulse radiolysis, superoxide dismutase, tryptophan
title Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
title_full Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
title_fullStr Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
title_full_unstemmed Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
title_short Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review
title_sort pulse radiolysis study of catalytic properties of human manganese superoxide dismutase a review
topic manganese, pulse radiolysis, superoxide dismutase, tryptophan
url https://sokjmls.com.ng/index.php/SJMLS/article/view/369
work_keys_str_mv AT alhassanajmuhammadiusulemsadamusmandumary pulseradiolysisstudyofcatalyticpropertiesofhumanmanganesesuperoxidedismutaseareview