Pulse Radiolysis Study of Catalytic Properties of Human Manganese Superoxide Dismutase: A Review

The depletion of superoxide catalyzed by human manganese superoxide dismutase (MnSOD) was observed spectrophotometrically by measuring the absorbance of superoxide at 250-280 nm following pulse radiolysis and it showed a biphasic pattern. Tryptophan 161 is a highly conserved residue that forms a hyd...

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Bibliographic Details
Main Author: Alhassan A. J.*, Muhammad, I. U., Sule, M.S, Adamu, S.M. and Umar, Y.
Format: Article
Language:English
Published: Hammer Head Production Limited 2016-07-01
Series:Sokoto Journal of Medical Laboratory Science
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Online Access:https://sokjmls.com.ng/index.php/SJMLS/article/view/369
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Summary:The depletion of superoxide catalyzed by human manganese superoxide dismutase (MnSOD) was observed spectrophotometrically by measuring the absorbance of superoxide at 250-280 nm following pulse radiolysis and it showed a biphasic pattern. Tryptophan 161 is a highly conserved residue that forms a hydrophobic side of the active site cavity of manganese superoxide dismutase (MnSOD), with its indole ring adjacent to and about 5 Å from the manganese. Trp 161 promotes the dissociation of product peroxide, perhaps in part through its effect on the orientation of Tyr 34.
ISSN:2536-7153