Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities

Coral reef survival is crucial for the socio-ecological interest of many countries, particularly for the Republic of Maldives, whose reef integrity influences the country’s livelihoods and economy. These ecosystems are being severely impacted by multiple stressors, leading to declines in biodiversit...

Full description

Saved in:
Bibliographic Details
Main Authors: Fortunato Palma Esposito, Andrea López-Mobilia, Michael Tangherlini, Vincenza Casella, Alessandro Coppola, Giulia Varola, Laura Vitale, Gerardo Della Sala, Pietro Tedesco, Simone Montano, Davide Seveso, Paolo Galli, Daniela Coppola, Donatella de Pascale, Christian Galasso
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biology
Subjects:
Online Access:https://www.mdpi.com/2079-7737/14/4/401
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850183285233156096
author Fortunato Palma Esposito
Andrea López-Mobilia
Michael Tangherlini
Vincenza Casella
Alessandro Coppola
Giulia Varola
Laura Vitale
Gerardo Della Sala
Pietro Tedesco
Simone Montano
Davide Seveso
Paolo Galli
Daniela Coppola
Donatella de Pascale
Christian Galasso
author_facet Fortunato Palma Esposito
Andrea López-Mobilia
Michael Tangherlini
Vincenza Casella
Alessandro Coppola
Giulia Varola
Laura Vitale
Gerardo Della Sala
Pietro Tedesco
Simone Montano
Davide Seveso
Paolo Galli
Daniela Coppola
Donatella de Pascale
Christian Galasso
author_sort Fortunato Palma Esposito
collection DOAJ
description Coral reef survival is crucial for the socio-ecological interest of many countries, particularly for the Republic of Maldives, whose reef integrity influences the country’s livelihoods and economy. These ecosystems are being severely impacted by multiple stressors, leading to declines in biodiversity. In the last few decades, researchers have focused on studying coral-associated microorganisms (CAMs) and their symbiotic role in coral health and resilience. Metabarcoding analysis has been widely utilized to study CAM diversity under various conditions but provides limited information on their functional roles. Therefore, cultivation of bacterial strains remains indispensable for validating ecological and biotechnological hypotheses. In this study, we investigated the microbial community associated with two abundant corals in Maldives, <i>Porites lobata</i> and <i>Acropora gemmifera</i>, and evaluated the antimicrobial, antioxidant, and UV-protectant properties of 10 promising isolated strains. The selected CAMs, <i>Pseudoalteromonas piscicida</i> 39, <i>Streptomyces parvus</i> 79, <i>Microbacterium</i> sp. 92 (a potential novel species), and <i>Micromonospora arenicola</i> 93, exhibited antibiotic activity against a panel of pathogenic strains (MIC from 0.01 to 500 µg/mL), antioxidant (comparable effect to that of Trolox and ascorbic acid), and UV-screen activities (protection of human keratinocytes at 200 µg/mL). Genomes revealed their dual potential in contributing to coral restoration and drug discovery strategies. These findings highlight the biotechnological relevance of CAMs, representing an important step toward the identification of novel and bioactive bacterial species beneficial for coral reef ecosystems and human health.
format Article
id doaj-art-428dd4de8515406ab12f3eae0d635e53
institution OA Journals
issn 2079-7737
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Biology
spelling doaj-art-428dd4de8515406ab12f3eae0d635e532025-08-20T02:17:25ZengMDPI AGBiology2079-77372025-04-0114440110.3390/biology14040401Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant ActivitiesFortunato Palma Esposito0Andrea López-Mobilia1Michael Tangherlini2Vincenza Casella3Alessandro Coppola4Giulia Varola5Laura Vitale6Gerardo Della Sala7Pietro Tedesco8Simone Montano9Davide Seveso10Paolo Galli11Daniela Coppola12Donatella de Pascale13Christian Galasso14Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Research Infrastructures for Marine Biological Resources, Stazione Zoologica Anton Dohrn, Fano Marine Centre, Viale Adriatico 1, 61032 Fano, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Earth and Environmental Science, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milano, ItalyDepartment of Earth and Environmental Science, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milano, ItalyDepartment of Earth and Environmental Science, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milano, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Ammiraglio Ferdinando Acton 55, 80133 Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Calabria Marine Centre, CRIMAC (Centro Ricerche ed Infrastrutture Marine Avanzate in Calabria), Stazione Zoologica Anton Dohrn, C. da Torre Spaccata, 87071 Amendolara, ItalyCoral reef survival is crucial for the socio-ecological interest of many countries, particularly for the Republic of Maldives, whose reef integrity influences the country’s livelihoods and economy. These ecosystems are being severely impacted by multiple stressors, leading to declines in biodiversity. In the last few decades, researchers have focused on studying coral-associated microorganisms (CAMs) and their symbiotic role in coral health and resilience. Metabarcoding analysis has been widely utilized to study CAM diversity under various conditions but provides limited information on their functional roles. Therefore, cultivation of bacterial strains remains indispensable for validating ecological and biotechnological hypotheses. In this study, we investigated the microbial community associated with two abundant corals in Maldives, <i>Porites lobata</i> and <i>Acropora gemmifera</i>, and evaluated the antimicrobial, antioxidant, and UV-protectant properties of 10 promising isolated strains. The selected CAMs, <i>Pseudoalteromonas piscicida</i> 39, <i>Streptomyces parvus</i> 79, <i>Microbacterium</i> sp. 92 (a potential novel species), and <i>Micromonospora arenicola</i> 93, exhibited antibiotic activity against a panel of pathogenic strains (MIC from 0.01 to 500 µg/mL), antioxidant (comparable effect to that of Trolox and ascorbic acid), and UV-screen activities (protection of human keratinocytes at 200 µg/mL). Genomes revealed their dual potential in contributing to coral restoration and drug discovery strategies. These findings highlight the biotechnological relevance of CAMs, representing an important step toward the identification of novel and bioactive bacterial species beneficial for coral reef ecosystems and human health.https://www.mdpi.com/2079-7737/14/4/401climate changecoral reefsMaldivescoral-associated microorganismsbioactivitygenome annotation
spellingShingle Fortunato Palma Esposito
Andrea López-Mobilia
Michael Tangherlini
Vincenza Casella
Alessandro Coppola
Giulia Varola
Laura Vitale
Gerardo Della Sala
Pietro Tedesco
Simone Montano
Davide Seveso
Paolo Galli
Daniela Coppola
Donatella de Pascale
Christian Galasso
Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
Biology
climate change
coral reefs
Maldives
coral-associated microorganisms
bioactivity
genome annotation
title Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
title_full Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
title_fullStr Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
title_full_unstemmed Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
title_short Novel Insights and Genomic Characterization of Coral-Associated Microorganisms from Maldives Displaying Antimicrobial, Antioxidant, and UV-Protectant Activities
title_sort novel insights and genomic characterization of coral associated microorganisms from maldives displaying antimicrobial antioxidant and uv protectant activities
topic climate change
coral reefs
Maldives
coral-associated microorganisms
bioactivity
genome annotation
url https://www.mdpi.com/2079-7737/14/4/401
work_keys_str_mv AT fortunatopalmaesposito novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT andrealopezmobilia novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT michaeltangherlini novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT vincenzacasella novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT alessandrocoppola novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT giuliavarola novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT lauravitale novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT gerardodellasala novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT pietrotedesco novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT simonemontano novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT davideseveso novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT paologalli novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT danielacoppola novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT donatelladepascale novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities
AT christiangalasso novelinsightsandgenomiccharacterizationofcoralassociatedmicroorganismsfrommaldivesdisplayingantimicrobialantioxidantanduvprotectantactivities