Preparation of Preformed Iodixanol Gradients
The Protocol Article describes the strategies for the formation of preformed discontinuous and continuous density gradients. Most of the techniques can be applied to the use of any gradient solute but some of the detailed recommendations on continuous gradients only apply to iodixanol gradients beca...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.285 |
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| _version_ | 1832560238940651520 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | The Protocol Article describes the strategies for the formation of preformed discontinuous and continuous density gradients. Most of the techniques can be applied to the use of any gradient solute but some of the detailed recommendations on continuous gradients only apply to iodixanol gradients because of variations in viscosity and rates of diffusion between different solute types. Operational variations that require consideration when dealing with specific types of biological particle are also dealt with at the end of this Protocol Article. |
| format | Article |
| id | doaj-art-42812c10e0ef4accae2ac4e8fb5d18e1 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-42812c10e0ef4accae2ac4e8fb5d18e12025-02-03T01:28:05ZengWileyThe Scientific World Journal1537-744X2002-01-0121351135510.1100/tsw.2002.285Preparation of Preformed Iodixanol GradientsJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKThe Protocol Article describes the strategies for the formation of preformed discontinuous and continuous density gradients. Most of the techniques can be applied to the use of any gradient solute but some of the detailed recommendations on continuous gradients only apply to iodixanol gradients because of variations in viscosity and rates of diffusion between different solute types. Operational variations that require consideration when dealing with specific types of biological particle are also dealt with at the end of this Protocol Article.http://dx.doi.org/10.1100/tsw.2002.285 |
| spellingShingle | John Graham Preparation of Preformed Iodixanol Gradients The Scientific World Journal |
| title | Preparation of Preformed Iodixanol Gradients |
| title_full | Preparation of Preformed Iodixanol Gradients |
| title_fullStr | Preparation of Preformed Iodixanol Gradients |
| title_full_unstemmed | Preparation of Preformed Iodixanol Gradients |
| title_short | Preparation of Preformed Iodixanol Gradients |
| title_sort | preparation of preformed iodixanol gradients |
| url | http://dx.doi.org/10.1100/tsw.2002.285 |
| work_keys_str_mv | AT johngraham preparationofpreformediodixanolgradients |