Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii

Whey beverage was prepared by utilizing Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 and Propionibacterium freudenreichii subsp. shermanii MTCC 1371 in order to make a fermented probiotic healthy drink. The product made with 4 % mixed culture (1:1:1) inoculated (initial count...

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Main Authors: T.K. Maity, Rakesh Kumar, A.K. Misra
Format: Article
Language:English
Published: Croatian Dairy Union 2008-12-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=48498
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author T.K. Maity
Rakesh Kumar
A.K. Misra
author_facet T.K. Maity
Rakesh Kumar
A.K. Misra
author_sort T.K. Maity
collection DOAJ
description Whey beverage was prepared by utilizing Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 and Propionibacterium freudenreichii subsp. shermanii MTCC 1371 in order to make a fermented probiotic healthy drink. The product made with 4 % mixed culture (1:1:1) inoculated (initial count - lactobacilli 6.2 x 107 CFU/mL, bifidobacteria 5.4 x 107 CFU/mL, propionibacteria 3.9 x 107 CFU/mL) in deprotienized whey (4.6 % lactose, 0.62 % ash, 0.48 % fat and 0.5 % protein) adjusted to pH 6.4 and incubated at 37 °C for 8 h has a good technological and dietetic criteria required for a probiotic product. Total bacterial count, lactobacilli count, bifidobacteria count, propionibacteria count, titratable acidity, β-D galactosidase activity, concentration of lactic acid and sensory properties were monitored during storage period. The whey beverage fermented for 8 h and prepared with 4 % inoculum of mixed culture (1:1:1) met the probiotic criterion by maintaining each type of bacterial population at counts greater than 108 CFU/mL up to 10 days of storage period. The titratable acidity as well as sensory properties did not change appreciably during first 7 days of storage. At the end of 15 days of storage, slight acidification was detected, although the beverage still retained an acceptable flavour.
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institution Kabale University
issn 0026-704X
1846-4025
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series Mljekarstvo
spelling doaj-art-427dd8a539274e29a6cd9368f5355fe62025-08-20T03:35:57ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252008-12-01584315325Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermaniiT.K. MaityRakesh KumarA.K. MisraWhey beverage was prepared by utilizing Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 and Propionibacterium freudenreichii subsp. shermanii MTCC 1371 in order to make a fermented probiotic healthy drink. The product made with 4 % mixed culture (1:1:1) inoculated (initial count - lactobacilli 6.2 x 107 CFU/mL, bifidobacteria 5.4 x 107 CFU/mL, propionibacteria 3.9 x 107 CFU/mL) in deprotienized whey (4.6 % lactose, 0.62 % ash, 0.48 % fat and 0.5 % protein) adjusted to pH 6.4 and incubated at 37 °C for 8 h has a good technological and dietetic criteria required for a probiotic product. Total bacterial count, lactobacilli count, bifidobacteria count, propionibacteria count, titratable acidity, β-D galactosidase activity, concentration of lactic acid and sensory properties were monitored during storage period. The whey beverage fermented for 8 h and prepared with 4 % inoculum of mixed culture (1:1:1) met the probiotic criterion by maintaining each type of bacterial population at counts greater than 108 CFU/mL up to 10 days of storage period. The titratable acidity as well as sensory properties did not change appreciably during first 7 days of storage. At the end of 15 days of storage, slight acidification was detected, although the beverage still retained an acceptable flavour.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=48498probioticwheyfermented beverageantibacterial activity
spellingShingle T.K. Maity
Rakesh Kumar
A.K. Misra
Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
Mljekarstvo
probiotic
whey
fermented beverage
antibacterial activity
title Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
title_full Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
title_fullStr Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
title_full_unstemmed Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
title_short Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii
title_sort development of healthy whey drink with lactobacillus rhamnosus bifidobacterium bifidum and propionibacterium freudenreichii subsp shermanii
topic probiotic
whey
fermented beverage
antibacterial activity
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=48498
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AT rakeshkumar developmentofhealthywheydrinkwithlactobacillusrhamnosusbifidobacteriumbifidumandpropionibacteriumfreudenreichiisubspshermanii
AT akmisra developmentofhealthywheydrinkwithlactobacillusrhamnosusbifidobacteriumbifidumandpropionibacteriumfreudenreichiisubspshermanii