Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes

Extracts from willow bark or leaves were shown to contain effective plant biostimulants and pharmaceutical compounds. Considering the limitation in the use of bark raw materials on large scale, here we analyzed the stimulatory effects of extracts from various organs of willow (Salix viminalis L) pla...

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Main Authors: Zoltán Zombori, Gabriella Szalai, Kamirán Áron Hamow, Miklós Hóvári, László Sass, Györgyi Ferenc, Tibor Janda, Dénes Dudits
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1650824/full
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author Zoltán Zombori
Gabriella Szalai
Kamirán Áron Hamow
Miklós Hóvári
László Sass
Györgyi Ferenc
Tibor Janda
Dénes Dudits
author_facet Zoltán Zombori
Gabriella Szalai
Kamirán Áron Hamow
Miklós Hóvári
László Sass
Györgyi Ferenc
Tibor Janda
Dénes Dudits
author_sort Zoltán Zombori
collection DOAJ
description Extracts from willow bark or leaves were shown to contain effective plant biostimulants and pharmaceutical compounds. Considering the limitation in the use of bark raw materials on large scale, here we analyzed the stimulatory effects of extracts from various organs of willow (Salix viminalis L) plants grown as short rotation shrub willow. Metabolomic analysis of water extracts from different organs (leaves, meristems, stems) of two triploid genotypes (Rába and Maros) revealed presence of bioactive compounds. Quantity of phenylpropanoids, flavones, flavonols, anthocyanins, aminobenzoate degradants, plant hormones and stylbenes varied between organs and genotypes. Several of these bioactive compounds are known as pharmaceuticals. Here we carried out phytostimulatory tests by using various extracts for seed priming and foliar spraying of maize plants in greenhouse and field experiments. Both the digital imagining of maize plants and measurements of the plant height showed variable stimulation of growth. Chlorophyll fluorescence parameters indicated improved photosynthetic performance. Increased seed weight/ear and 1000 seed weight (17%) were detected after foliar spraying. The present study supports the extended application of bioactive phytocompounds by offering a novel raw material, the short rotation willow shoots as sources of bioactive compounds for use in agricultural practice and pharmaceutical industry.
format Article
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issn 1664-462X
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publishDate 2025-08-01
publisher Frontiers Media S.A.
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series Frontiers in Plant Science
spelling doaj-art-427723e231ef4c92b97e874131a674ca2025-08-20T03:23:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.16508241650824Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexesZoltán Zombori0Gabriella Szalai1Kamirán Áron Hamow2Miklós Hóvári3László Sass4Györgyi Ferenc5Tibor Janda6Dénes Dudits7Institute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryDepartment of Plant Physiology and Metabolomics, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, HungaryDepartment of Plant Physiology and Metabolomics, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, HungaryInstitute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstitute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstitute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryDepartment of Plant Physiology and Metabolomics, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, HungaryInstitute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryExtracts from willow bark or leaves were shown to contain effective plant biostimulants and pharmaceutical compounds. Considering the limitation in the use of bark raw materials on large scale, here we analyzed the stimulatory effects of extracts from various organs of willow (Salix viminalis L) plants grown as short rotation shrub willow. Metabolomic analysis of water extracts from different organs (leaves, meristems, stems) of two triploid genotypes (Rába and Maros) revealed presence of bioactive compounds. Quantity of phenylpropanoids, flavones, flavonols, anthocyanins, aminobenzoate degradants, plant hormones and stylbenes varied between organs and genotypes. Several of these bioactive compounds are known as pharmaceuticals. Here we carried out phytostimulatory tests by using various extracts for seed priming and foliar spraying of maize plants in greenhouse and field experiments. Both the digital imagining of maize plants and measurements of the plant height showed variable stimulation of growth. Chlorophyll fluorescence parameters indicated improved photosynthetic performance. Increased seed weight/ear and 1000 seed weight (17%) were detected after foliar spraying. The present study supports the extended application of bioactive phytocompounds by offering a novel raw material, the short rotation willow shoots as sources of bioactive compounds for use in agricultural practice and pharmaceutical industry.https://www.frontiersin.org/articles/10.3389/fpls.2025.1650824/fullactive phytobiological metabolitesLC-MS/MSmetabolomic pathwaysSalix sp.seed primingspraying
spellingShingle Zoltán Zombori
Gabriella Szalai
Kamirán Áron Hamow
Miklós Hóvári
László Sass
Györgyi Ferenc
Tibor Janda
Dénes Dudits
Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
Frontiers in Plant Science
active phytobiological metabolites
LC-MS/MS
metabolomic pathways
Salix sp.
seed priming
spraying
title Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
title_full Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
title_fullStr Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
title_full_unstemmed Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
title_short Bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
title_sort bioactive compounds in extracts from short rotation willow shoots known as pharmaceuticals and experimental demonstration of biostimulation of maize plants by these chemical complexes
topic active phytobiological metabolites
LC-MS/MS
metabolomic pathways
Salix sp.
seed priming
spraying
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1650824/full
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