Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders
In this study, a straightforward and cost-effective HPLC-UV method was developed for the rapid determination of vitamin D<sub>2</sub> and ergosterol in mushrooms. These bioactive components are known to play a significant role in the nutritional value of mushrooms, particularly in the pr...
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2025-04-01
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| author | Judit Bajzát András Misz József Rácz Máté Vágvölgyi Csaba Csutorás Csaba Vágvölgyi |
| author_facet | Judit Bajzát András Misz József Rácz Máté Vágvölgyi Csaba Csutorás Csaba Vágvölgyi |
| author_sort | Judit Bajzát |
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| description | In this study, a straightforward and cost-effective HPLC-UV method was developed for the rapid determination of vitamin D<sub>2</sub> and ergosterol in mushrooms. These bioactive components are known to play a significant role in the nutritional value of mushrooms, particularly in the production of mushroom-based food supplements. The method, designed for routine analysis, involves a simple sample preparation process combining saponification and solid–liquid extraction, followed by HPLC-UV detection. High recovery rates (97–99%) were achieved by the method, with limits of detection (LOD) and quantitation (LOQ) of 0.1 mg/kg dry weight and 0.5 mg/kg dry weight, respectively. The enrichment of vitamin D₂ content in mushroom powders through UV irradiation was also investigated. In <i>Agaricus bisporus</i>, vitamin D₂ levels increased from an initial 1.92 mg/kg to 4.66 mg/kg following heat treatment at 100 °C, and reached a maximum of 28.13 mg/kg when heat treatment was combined with UV irradiation. In contrast, <i>Lentinula edodes</i> exhibited an initial vitamin D₂ content of 7–8.5 mg/kg, with the highest levels achieved through UV treatment alone, which also preserved ergosterol content. These findings highlight species-specific differences in vitamin D₂ conversion and present an effective approach for enhancing the nutritional profile of mushroom-based products, while providing a reliable analytical tool for quality control. |
| format | Article |
| id | doaj-art-42769083cd1d4c5f931bb02548f2af77 |
| institution | DOAJ |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-42769083cd1d4c5f931bb02548f2af772025-08-20T03:08:44ZengMDPI AGApplied Sciences2076-34172025-04-01157405810.3390/app15074058Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom PowdersJudit Bajzát0András Misz1József Rácz2Máté Vágvölgyi3Csaba Csutorás4Csaba Vágvölgyi5Új Champignons Ltd., Bartók Béla Str. 162, H-1224 Budapest, HungaryÚj Champignons Ltd., Bartók Béla Str. 162, H-1224 Budapest, HungaryMagyar Gomba Kertész Ltd., Bartók Béla Str. 162, H-1224 Budapest, HungaryInstitute of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Zrínyi Str. 9, H-6720 Szeged, HungaryÚj Champignons Ltd., Bartók Béla Str. 162, H-1224 Budapest, HungaryDepartment of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, HungaryIn this study, a straightforward and cost-effective HPLC-UV method was developed for the rapid determination of vitamin D<sub>2</sub> and ergosterol in mushrooms. These bioactive components are known to play a significant role in the nutritional value of mushrooms, particularly in the production of mushroom-based food supplements. The method, designed for routine analysis, involves a simple sample preparation process combining saponification and solid–liquid extraction, followed by HPLC-UV detection. High recovery rates (97–99%) were achieved by the method, with limits of detection (LOD) and quantitation (LOQ) of 0.1 mg/kg dry weight and 0.5 mg/kg dry weight, respectively. The enrichment of vitamin D₂ content in mushroom powders through UV irradiation was also investigated. In <i>Agaricus bisporus</i>, vitamin D₂ levels increased from an initial 1.92 mg/kg to 4.66 mg/kg following heat treatment at 100 °C, and reached a maximum of 28.13 mg/kg when heat treatment was combined with UV irradiation. In contrast, <i>Lentinula edodes</i> exhibited an initial vitamin D₂ content of 7–8.5 mg/kg, with the highest levels achieved through UV treatment alone, which also preserved ergosterol content. These findings highlight species-specific differences in vitamin D₂ conversion and present an effective approach for enhancing the nutritional profile of mushroom-based products, while providing a reliable analytical tool for quality control.https://www.mdpi.com/2076-3417/15/7/4058vitamin D<sub>2</sub>UV irradiationbutton mushroomshiitake mushroomliquid chromatography |
| spellingShingle | Judit Bajzát András Misz József Rácz Máté Vágvölgyi Csaba Csutorás Csaba Vágvölgyi Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders Applied Sciences vitamin D<sub>2</sub> UV irradiation button mushroom shiitake mushroom liquid chromatography |
| title | Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders |
| title_full | Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders |
| title_fullStr | Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders |
| title_full_unstemmed | Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders |
| title_short | Development of a Simple HPLC Method for the Analysis of Ergosterol and UV-Enriched Vitamin D₂ in Mushroom Powders |
| title_sort | development of a simple hplc method for the analysis of ergosterol and uv enriched vitamin d₂ in mushroom powders |
| topic | vitamin D<sub>2</sub> UV irradiation button mushroom shiitake mushroom liquid chromatography |
| url | https://www.mdpi.com/2076-3417/15/7/4058 |
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