Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet
The use of chitosan (CHI) in ruminant diets is a promising natural modifier for rumen fermentation, capable of modulating both the rumen pattern and microbial activities. The objective of this study was to explore the rumen fermentation and microbial populations in Dhofari goats fed a diet supplemen...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2337748 |
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| author | Hani M. El-Zaiat Waleed Al-Marzooqi Kaadhia Al-Kharousi |
| author_facet | Hani M. El-Zaiat Waleed Al-Marzooqi Kaadhia Al-Kharousi |
| author_sort | Hani M. El-Zaiat |
| collection | DOAJ |
| description | The use of chitosan (CHI) in ruminant diets is a promising natural modifier for rumen fermentation, capable of modulating both the rumen pattern and microbial activities. The objective of this study was to explore the rumen fermentation and microbial populations in Dhofari goats fed a diet supplemented with CHI. A total of 24 Dhofari lactating goats (body weight, 27.32 ± 1.80 kg) were assigned randomly into three experimental groups (n = 8 ewes/group). Goats were fed a basal diet with either 0 (control), 180 (low), or 360 (high) mg CHI/kg of dietary dry matter (DM) for 45 days. Feeding high CHI linearly increased (p < 0.05) the propionate level and reduced the acetate, butyrate, and total protozoa count (p < 0.05). Ruminal ammonia nitrogen (NH3–N) concentrations and the acetate:propionate ratio decreased linearly when goats were fed CHI (p < 0.05). The abundances of both Spirochetes and Fibrobacteres phyla were reduced (p < 0.05) with both CHI doses relative to the control. Both low and high CHI reduced (p < 0.05) the relative abundances of Butyrivibrio hungatei, Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, Selenomonas ruminantium and Neocallimastix californiae populations. Adding CHI significantly decreased (p < 0.05) the abundances of Ascomycota, Basidiomycota, and Bacillariophyta phyla compared to the control. Adding CHI to the diet reduces the abundance of fibrolytic-degrading bacteria, however, it increases the amylolytic-degrading bacteria. Application of 360 mg of CHI/kg DM modified the relative populations of ruminal microbes, which could enhance the rumen fermentation patterns in Dhofari goats. |
| format | Article |
| id | doaj-art-42723894b9c74ff08b67946d34ca3e08 |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-42723894b9c74ff08b67946d34ca3e082025-08-20T03:55:54ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2337748Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added dietHani M. El-Zaiat0Waleed Al-Marzooqi1Kaadhia Al-Kharousi2Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Sultanate of OmanDepartment of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Sultanate of OmanDepartment of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Sultanate of OmanThe use of chitosan (CHI) in ruminant diets is a promising natural modifier for rumen fermentation, capable of modulating both the rumen pattern and microbial activities. The objective of this study was to explore the rumen fermentation and microbial populations in Dhofari goats fed a diet supplemented with CHI. A total of 24 Dhofari lactating goats (body weight, 27.32 ± 1.80 kg) were assigned randomly into three experimental groups (n = 8 ewes/group). Goats were fed a basal diet with either 0 (control), 180 (low), or 360 (high) mg CHI/kg of dietary dry matter (DM) for 45 days. Feeding high CHI linearly increased (p < 0.05) the propionate level and reduced the acetate, butyrate, and total protozoa count (p < 0.05). Ruminal ammonia nitrogen (NH3–N) concentrations and the acetate:propionate ratio decreased linearly when goats were fed CHI (p < 0.05). The abundances of both Spirochetes and Fibrobacteres phyla were reduced (p < 0.05) with both CHI doses relative to the control. Both low and high CHI reduced (p < 0.05) the relative abundances of Butyrivibrio hungatei, Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, Selenomonas ruminantium and Neocallimastix californiae populations. Adding CHI significantly decreased (p < 0.05) the abundances of Ascomycota, Basidiomycota, and Bacillariophyta phyla compared to the control. Adding CHI to the diet reduces the abundance of fibrolytic-degrading bacteria, however, it increases the amylolytic-degrading bacteria. Application of 360 mg of CHI/kg DM modified the relative populations of ruminal microbes, which could enhance the rumen fermentation patterns in Dhofari goats.https://www.tandfonline.com/doi/10.1080/10495398.2024.2337748Chitosan additiverumen fermentationmicrobiome sequencingDhofari goats |
| spellingShingle | Hani M. El-Zaiat Waleed Al-Marzooqi Kaadhia Al-Kharousi Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet Animal Biotechnology Chitosan additive rumen fermentation microbiome sequencing Dhofari goats |
| title | Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet |
| title_full | Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet |
| title_fullStr | Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet |
| title_full_unstemmed | Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet |
| title_short | Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet |
| title_sort | exploring rumen fermentation and microbial populations in dhofari goats fed a chitosan added diet |
| topic | Chitosan additive rumen fermentation microbiome sequencing Dhofari goats |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2337748 |
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