PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer
Cyclin B1 is an essential cyclin-dependent protein that involves in the G2/M transition. Multiple studies report that cyclin B1 is upregulated in cancers and promotes cancer progression. However, the mechanism of cyclin B1 upregulation remains unclear. Here we report that the 5′UTR of cyclin B1 mRNA...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2022-05-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022046 |
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| _version_ | 1849737014292774912 |
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| author | Fan Xinyi Zhao Zitong Ma Liying Huang Xuanlin Zhan Qimin Song Yongmei |
| author_facet | Fan Xinyi Zhao Zitong Ma Liying Huang Xuanlin Zhan Qimin Song Yongmei |
| author_sort | Fan Xinyi |
| collection | DOAJ |
| description | Cyclin B1 is an essential cyclin-dependent protein that involves in the G2/M transition. Multiple studies report that cyclin B1 is upregulated in cancers and promotes cancer progression. However, the mechanism of cyclin B1 upregulation remains unclear. Here we report that the 5′UTR of cyclin B1 mRNA contains an internal ribosome entry site (IRES) by using a bicistronic fluorescent reporter. We show that IRES can initiate the translation of cyclin B1, and the IRES-mediated translation is further activated under cell stress. Interacting trans-acting factors (ITAFs) are required by most IRES to initiate the translation. We find that PTBP1 promotes the IRES-mediated translation of cyclin B1 by binding to the 5′UTR of cyclin B1. On top of that, PTBP1 promotes the malignancy of ESCC cells. Our data suggest that the IRES-mediated translation of cyclin B1 plays an essential role in the cyclin B1 upregulation in cancers. |
| format | Article |
| id | doaj-art-425f460973214efd80a5b6bf2bc74774 |
| institution | DOAJ |
| issn | 1672-9145 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-425f460973214efd80a5b6bf2bc747742025-08-20T03:07:05ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-05-015469670710.3724/abbs.202204620d259ccPTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancerFan Xinyi0Zhao Zitong1Ma Liying2Huang Xuanlin3Zhan Qimin4Song Yongmei5["State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 China","Department of Radiation Oncology, Weill Cornell Medical College, New York, NY 10065, USA"]["State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 China"]["State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 China"]["BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China"]["State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 China","Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China"]["State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021 China"]Cyclin B1 is an essential cyclin-dependent protein that involves in the G2/M transition. Multiple studies report that cyclin B1 is upregulated in cancers and promotes cancer progression. However, the mechanism of cyclin B1 upregulation remains unclear. Here we report that the 5′UTR of cyclin B1 mRNA contains an internal ribosome entry site (IRES) by using a bicistronic fluorescent reporter. We show that IRES can initiate the translation of cyclin B1, and the IRES-mediated translation is further activated under cell stress. Interacting trans-acting factors (ITAFs) are required by most IRES to initiate the translation. We find that PTBP1 promotes the IRES-mediated translation of cyclin B1 by binding to the 5′UTR of cyclin B1. On top of that, PTBP1 promotes the malignancy of ESCC cells. Our data suggest that the IRES-mediated translation of cyclin B1 plays an essential role in the cyclin B1 upregulation in cancers.https://www.sciengine.com/doi/10.3724/abbs.2022046cyclin B1internal ribosome entry sitePTBP1interacting trans-acting factorsesophageal squamous cell carcinoma |
| spellingShingle | Fan Xinyi Zhao Zitong Ma Liying Huang Xuanlin Zhan Qimin Song Yongmei PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer Acta Biochimica et Biophysica Sinica cyclin B1 internal ribosome entry site PTBP1 interacting trans-acting factors esophageal squamous cell carcinoma |
| title | PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer |
| title_full | PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer |
| title_fullStr | PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer |
| title_full_unstemmed | PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer |
| title_short | PTBP1 promotes IRES-mediated translation of cyclin B1<?A3B2 ACK?>in cancer |
| title_sort | ptbp1 promotes ires mediated translation of cyclin b1 a3b2 ack in cancer |
| topic | cyclin B1 internal ribosome entry site PTBP1 interacting trans-acting factors esophageal squamous cell carcinoma |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022046 |
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