Outer hair cells stir cochlear fluids
We hypothesized that active outer hair cells drive cochlear fluid circulation. The hypothesis was tested by delivering the neurotoxin, kainic acid, to the intact round window of young gerbil cochleae while monitoring auditory responses in the cochlear nucleus. Sounds presented at a modest level sign...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2025-01-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/101943 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526637032636416 |
|---|---|
| author | Choongheon Lee Mohammad Shokrian Kenneth S Henry Laurel H Carney J Christopher Holt Jong-Hoon Nam |
| author_facet | Choongheon Lee Mohammad Shokrian Kenneth S Henry Laurel H Carney J Christopher Holt Jong-Hoon Nam |
| author_sort | Choongheon Lee |
| collection | DOAJ |
| description | We hypothesized that active outer hair cells drive cochlear fluid circulation. The hypothesis was tested by delivering the neurotoxin, kainic acid, to the intact round window of young gerbil cochleae while monitoring auditory responses in the cochlear nucleus. Sounds presented at a modest level significantly expedited kainic acid delivery. When outer-hair-cell motility was suppressed by salicylate, the facilitation effect was compromised. A low-frequency tone was more effective than broadband noise, especially for drug delivery to apical locations. Computational model simulations provided the physical basis for our observation, which incorporated solute diffusion, fluid advection, fluid–structure interaction, and outer-hair-cell motility. Active outer hair cells deformed the organ of Corti like a peristaltic tube to generate apically streaming flows along the tunnel of Corti and basally streaming flows along the scala tympani. Our measurements and simulations coherently suggest that active outer hair cells in the tail region of cochlear traveling waves drive cochlear fluid circulation. |
| format | Article |
| id | doaj-art-4251cc2cdd8741d298999d423141d9f6 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-4251cc2cdd8741d298999d423141d9f62025-01-16T13:15:36ZengeLife Sciences Publications LtdeLife2050-084X2025-01-011310.7554/eLife.101943Outer hair cells stir cochlear fluidsChoongheon Lee0https://orcid.org/0000-0002-3080-681XMohammad Shokrian1https://orcid.org/0000-0003-0360-323XKenneth S Henry2https://orcid.org/0000-0003-1364-318XLaurel H Carney3https://orcid.org/0000-0002-4729-5702J Christopher Holt4https://orcid.org/0000-0001-7908-083XJong-Hoon Nam5https://orcid.org/0000-0002-7477-5453Department of Otolaryngology, University of Rochester, Rochester, United States; Department of Mechanical Engineering, University of Rochester, Rochester, United StatesDepartment of Mechanical Engineering, University of Rochester, Rochester, United StatesDepartment of Otolaryngology, University of Rochester, Rochester, United States; Department of Biomedical Engineering, University of Rochester, Rochester, United States; Department of Neuroscience, University of Rochester, Rochester, United StatesDepartment of Biomedical Engineering, University of Rochester, Rochester, United States; Department of Neuroscience, University of Rochester, Rochester, United States; Department of Electrical and Computer Engineering, University of Rochester, Rochester, United StatesDepartment of Otolaryngology, University of Rochester, Rochester, United States; Department of Neuroscience, University of Rochester, Rochester, United StatesDepartment of Mechanical Engineering, University of Rochester, Rochester, United States; Department of Biomedical Engineering, University of Rochester, Rochester, United States; Department of Neuroscience, University of Rochester, Rochester, United StatesWe hypothesized that active outer hair cells drive cochlear fluid circulation. The hypothesis was tested by delivering the neurotoxin, kainic acid, to the intact round window of young gerbil cochleae while monitoring auditory responses in the cochlear nucleus. Sounds presented at a modest level significantly expedited kainic acid delivery. When outer-hair-cell motility was suppressed by salicylate, the facilitation effect was compromised. A low-frequency tone was more effective than broadband noise, especially for drug delivery to apical locations. Computational model simulations provided the physical basis for our observation, which incorporated solute diffusion, fluid advection, fluid–structure interaction, and outer-hair-cell motility. Active outer hair cells deformed the organ of Corti like a peristaltic tube to generate apically streaming flows along the tunnel of Corti and basally streaming flows along the scala tympani. Our measurements and simulations coherently suggest that active outer hair cells in the tail region of cochlear traveling waves drive cochlear fluid circulation.https://elifesciences.org/articles/101943gerbilcochleaouter hair celldrug deliveryorgan of Cortikainic acid |
| spellingShingle | Choongheon Lee Mohammad Shokrian Kenneth S Henry Laurel H Carney J Christopher Holt Jong-Hoon Nam Outer hair cells stir cochlear fluids eLife gerbil cochlea outer hair cell drug delivery organ of Corti kainic acid |
| title | Outer hair cells stir cochlear fluids |
| title_full | Outer hair cells stir cochlear fluids |
| title_fullStr | Outer hair cells stir cochlear fluids |
| title_full_unstemmed | Outer hair cells stir cochlear fluids |
| title_short | Outer hair cells stir cochlear fluids |
| title_sort | outer hair cells stir cochlear fluids |
| topic | gerbil cochlea outer hair cell drug delivery organ of Corti kainic acid |
| url | https://elifesciences.org/articles/101943 |
| work_keys_str_mv | AT choongheonlee outerhaircellsstircochlearfluids AT mohammadshokrian outerhaircellsstircochlearfluids AT kennethshenry outerhaircellsstircochlearfluids AT laurelhcarney outerhaircellsstircochlearfluids AT jchristopherholt outerhaircellsstircochlearfluids AT jonghoonnam outerhaircellsstircochlearfluids |