Role of ENaC in gender-associated differences in blood pressure
Objective(s): Sexual dimorphism in blood pressure regulation has been extensively noted in humans, but the underlying mechanisms remain to be fully understood. Our research aims to investigate the possible correlation between gender-associated differences in blood pressure and renal sodium transport...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-04-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_25443_d3a2fdc7280854f6ebd57e55669e1e51.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849252070647922688 |
|---|---|
| author | Guo-feng Yu Li-qin Yu Qin-rui Lai Wei Li |
| author_facet | Guo-feng Yu Li-qin Yu Qin-rui Lai Wei Li |
| author_sort | Guo-feng Yu |
| collection | DOAJ |
| description | Objective(s): Sexual dimorphism in blood pressure regulation has been extensively noted in humans, but the underlying mechanisms remain to be fully understood. Our research aims to investigate the possible correlation between gender-associated differences in blood pressure and renal sodium transport. Materials and Methods: We measured male and female mice’s blood pressure, urine, and plasma sodium concentration when fed a regular or high-Na+ diet. After that, their renal sodium transporters were assessed by western blot and immunofluorescence. For further investigation, male mice were castrated to observe the differences in blood pressure and renal sodium transporters compared to normal mice. Results: Male mice exhibited higher blood pressure and lower renal sodium excretion than female littermates. Furthermore, the blood pressure of male mice exhibited a more significant and rapid increase relative to female mice when the diet was switched from control sodium to high sodium. Western blot and immunofluorescent staining revealed that in male mice, the sodium transporters epithelial sodium channel (ENaC) and the upstream kinases SPAK (Ste20-related proline/alanine-rich kinase), OSR1 (oxidative stress response kinase 1), and WNK4 (Lysine-Deficient Protein Kinase 4) were elevated. Beyond that, male mice exhibited lowered blood pressure and reduced abundance of ENaC (α, β, and γ) after castration.Conclusion: ENaC plays a significant role in gender-associated differences in blood pressure and renal sodium reabsorption. |
| format | Article |
| id | doaj-art-424dc1027dfd462cb750a198c2388c8e |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-424dc1027dfd462cb750a198c2388c8e2025-08-20T03:56:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-04-0128452753210.22038/ijbms.2025.81832.1770125443Role of ENaC in gender-associated differences in blood pressureGuo-feng Yu0Li-qin Yu1Qin-rui Lai2Wei Li3Department of Clinical Laboratory, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China, 310052Qiaosi Branch, First People’s Hospital of Linping District, Hangzhou, Zhejiang Province, ChinaDepartment of Clinical Laboratory, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China, 310052Department of Clinical Laboratory, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China, 310052Objective(s): Sexual dimorphism in blood pressure regulation has been extensively noted in humans, but the underlying mechanisms remain to be fully understood. Our research aims to investigate the possible correlation between gender-associated differences in blood pressure and renal sodium transport. Materials and Methods: We measured male and female mice’s blood pressure, urine, and plasma sodium concentration when fed a regular or high-Na+ diet. After that, their renal sodium transporters were assessed by western blot and immunofluorescence. For further investigation, male mice were castrated to observe the differences in blood pressure and renal sodium transporters compared to normal mice. Results: Male mice exhibited higher blood pressure and lower renal sodium excretion than female littermates. Furthermore, the blood pressure of male mice exhibited a more significant and rapid increase relative to female mice when the diet was switched from control sodium to high sodium. Western blot and immunofluorescent staining revealed that in male mice, the sodium transporters epithelial sodium channel (ENaC) and the upstream kinases SPAK (Ste20-related proline/alanine-rich kinase), OSR1 (oxidative stress response kinase 1), and WNK4 (Lysine-Deficient Protein Kinase 4) were elevated. Beyond that, male mice exhibited lowered blood pressure and reduced abundance of ENaC (α, β, and γ) after castration.Conclusion: ENaC plays a significant role in gender-associated differences in blood pressure and renal sodium reabsorption.https://ijbms.mums.ac.ir/article_25443_d3a2fdc7280854f6ebd57e55669e1e51.pdfcastrationepithelial sodium channelhypertensionlysine-deficient protein - kinasesex differences |
| spellingShingle | Guo-feng Yu Li-qin Yu Qin-rui Lai Wei Li Role of ENaC in gender-associated differences in blood pressure Iranian Journal of Basic Medical Sciences castration epithelial sodium channel hypertension lysine-deficient protein - kinase sex differences |
| title | Role of ENaC in gender-associated differences in blood pressure |
| title_full | Role of ENaC in gender-associated differences in blood pressure |
| title_fullStr | Role of ENaC in gender-associated differences in blood pressure |
| title_full_unstemmed | Role of ENaC in gender-associated differences in blood pressure |
| title_short | Role of ENaC in gender-associated differences in blood pressure |
| title_sort | role of enac in gender associated differences in blood pressure |
| topic | castration epithelial sodium channel hypertension lysine-deficient protein - kinase sex differences |
| url | https://ijbms.mums.ac.ir/article_25443_d3a2fdc7280854f6ebd57e55669e1e51.pdf |
| work_keys_str_mv | AT guofengyu roleofenacingenderassociateddifferencesinbloodpressure AT liqinyu roleofenacingenderassociateddifferencesinbloodpressure AT qinruilai roleofenacingenderassociateddifferencesinbloodpressure AT weili roleofenacingenderassociateddifferencesinbloodpressure |