Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma

Cytoplasmic p27 plays an important role in regulating the cell cycle. Recent studies have revealed p27 protein translocation from the nucleus to the cytoplasm in many tumour cells. The aim of this study was to investigate the role and molecular mechanisms of cytoplasmic p27 in the progression of nas...

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Main Authors: Yaoshu Teng, Linping Hu, Bo Yu, Xiao Li, Manman Chen, Xiao Fu, Jin Zhang, Yueqiu Gao, Rujun Xu, Jin Zhu
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Artificial Cells, Nanomedicine, and Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/21691401.2019.1699811
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author Yaoshu Teng
Linping Hu
Bo Yu
Xiao Li
Manman Chen
Xiao Fu
Jin Zhang
Yueqiu Gao
Rujun Xu
Jin Zhu
author_facet Yaoshu Teng
Linping Hu
Bo Yu
Xiao Li
Manman Chen
Xiao Fu
Jin Zhang
Yueqiu Gao
Rujun Xu
Jin Zhu
author_sort Yaoshu Teng
collection DOAJ
description Cytoplasmic p27 plays an important role in regulating the cell cycle. Recent studies have revealed p27 protein translocation from the nucleus to the cytoplasm in many tumour cells. The aim of this study was to investigate the role and molecular mechanisms of cytoplasmic p27 in the progression of nasopharyngeal carcinoma (NPC) and to explore its prognostic value. We found increased cytoplasmic p27 expression by immunohistochemistry in NPC tissues, and its expression level was significantly correlated with the T classification and TNM clinical stage of NPC. The survival rate was significantly lower for NPC patients with cytoplasmic p27 immunopositivity than for NPC patients with cytoplasmic p27 immunonegativity, and cytoplasmic p27 was an independent risk factor that affected the prognosis of patients with NPC. Cytoplasmic p27 promoted the proliferation, cell cycle progression, migration, and invasion of NPC cells, increased Bim-1 and Twist1 protein levels, and decreased RhoA-GTP level. Collectively, these findings suggest that cytoplasmic relocalization of p27 is involved in the pathogenesis of NPC and is closely related to the unfavourable prognosis of patients with NPC. Therefore, cytoplasmic p27 might be a useful prognostic factor and potential therapeutic target for patients with NPC.
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institution Kabale University
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publisher Taylor & Francis Group
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series Artificial Cells, Nanomedicine, and Biotechnology
spelling doaj-art-42244ab389cc4e02b01f69d63ad896f02025-08-20T03:41:30ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148133634410.1080/21691401.2019.1699811Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinomaYaoshu Teng0Linping Hu1Bo Yu2Xiao Li3Manman Chen4Xiao Fu5Jin Zhang6Yueqiu Gao7Rujun Xu8Jin Zhu9Department of Otorhinolaryngology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaDepartment of Otorhinolaryngology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaDepartment of Otorhinolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, P.R. ChinaDepartment of Pathology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaDepartment of Otorhinolaryngology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaThe Fourth Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, P.R. ChinaThe Fourth Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, P.R. ChinaDepartment of Otorhinolaryngology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaDepartment of Pathology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaDepartment of Otorhinolaryngology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. ChinaCytoplasmic p27 plays an important role in regulating the cell cycle. Recent studies have revealed p27 protein translocation from the nucleus to the cytoplasm in many tumour cells. The aim of this study was to investigate the role and molecular mechanisms of cytoplasmic p27 in the progression of nasopharyngeal carcinoma (NPC) and to explore its prognostic value. We found increased cytoplasmic p27 expression by immunohistochemistry in NPC tissues, and its expression level was significantly correlated with the T classification and TNM clinical stage of NPC. The survival rate was significantly lower for NPC patients with cytoplasmic p27 immunopositivity than for NPC patients with cytoplasmic p27 immunonegativity, and cytoplasmic p27 was an independent risk factor that affected the prognosis of patients with NPC. Cytoplasmic p27 promoted the proliferation, cell cycle progression, migration, and invasion of NPC cells, increased Bim-1 and Twist1 protein levels, and decreased RhoA-GTP level. Collectively, these findings suggest that cytoplasmic relocalization of p27 is involved in the pathogenesis of NPC and is closely related to the unfavourable prognosis of patients with NPC. Therefore, cytoplasmic p27 might be a useful prognostic factor and potential therapeutic target for patients with NPC.https://www.tandfonline.com/doi/10.1080/21691401.2019.1699811p27biomarkernasopharyngeal carcinomaprognosis
spellingShingle Yaoshu Teng
Linping Hu
Bo Yu
Xiao Li
Manman Chen
Xiao Fu
Jin Zhang
Yueqiu Gao
Rujun Xu
Jin Zhu
Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
Artificial Cells, Nanomedicine, and Biotechnology
p27
biomarker
nasopharyngeal carcinoma
prognosis
title Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
title_full Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
title_fullStr Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
title_full_unstemmed Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
title_short Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
title_sort cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma
topic p27
biomarker
nasopharyngeal carcinoma
prognosis
url https://www.tandfonline.com/doi/10.1080/21691401.2019.1699811
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