Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells
Lung cancer remains a leading cause of cancer-related mortality, necessitating novel therapeutic strategies. Aristolochia bracteolata Lam. has been traditionally studied for its potential in cancer treatment, and this study explores the green synthesis of silver nanoparticles (AgNPs) using its ethyl...
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Elsevier
2025-01-01
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| Series: | Next Nanotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949829525000439 |
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| author | H.S. Anil Kumar Santosh Mallikarjun Bhavi Sapam Riches Singh Bothe Thokchom Ramesh Babu Yarajarla D. Kotresha |
| author_facet | H.S. Anil Kumar Santosh Mallikarjun Bhavi Sapam Riches Singh Bothe Thokchom Ramesh Babu Yarajarla D. Kotresha |
| author_sort | H.S. Anil Kumar |
| collection | DOAJ |
| description | Lung cancer remains a leading cause of cancer-related mortality, necessitating novel therapeutic strategies. Aristolochia bracteolata Lam. has been traditionally studied for its potential in cancer treatment, and this study explores the green synthesis of silver nanoparticles (AgNPs) using its ethyl acetate extract to evaluate their anticancer potential against human lung adenocarcinoma (NCIH-460) cells. AgNPs biosynthesis was confirmed by a color change and characterized by UV-Vis spectroscopy, FTIR, XRD, zeta potential FE-SEM, and TEM. The UV-Vis spectrum exhibited a characteristic absorption peak at 450 nm, confirming nanoparticle formation. XRD analysis indicated the crystalline nature of AgNPs, while FTIR spectra identified key functional groups involved in nanoparticle stabilization, including O-H, C-H, CC, and C-O bonds. FE-SEM and TEM images showed polydispersed, mostly spherical AgNPs ranging from 4 to 171 nm, with an average TEM-derived size of 10 nm. Zeta potential analysis showed a charge of −26 mV, suggesting moderate stability in solution. The AgNPs exhibited potent cytotoxicity against NCIH-460 cells (IC₅₀ = 13.44 µg mL−1) while sparing normal L929 cells (IC₅₀ = 196.05 µg mL−1). Annexin V/PI and TUNEL assays confirmed apoptosis induction, while cell cycle analysis demonstrated G0/G1 phase arrest, supported by caspase-3 upregulation. These findings suggest biosynthesized AgNPs as promising anticancer agents, capable of inducing apoptosis and disrupting cancer cell proliferation, making them potential candidates for lung cancer therapy. |
| format | Article |
| id | doaj-art-421d238c7b5c4d7296a2cb352d81e3a0 |
| institution | OA Journals |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Next Nanotechnology |
| spelling | doaj-art-421d238c7b5c4d7296a2cb352d81e3a02025-08-20T02:10:03ZengElsevierNext Nanotechnology2949-82952025-01-01710017410.1016/j.nxnano.2025.100174Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cellsH.S. Anil Kumar0Santosh Mallikarjun Bhavi1Sapam Riches Singh2Bothe Thokchom3Ramesh Babu Yarajarla4D. Kotresha5Department of Studies in Botany, Davangere University, Shivagangothri, Davangere, Karnataka 577507, IndiaDrosophila and Nano-biomedicine Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nano-biomedicine Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nano-biomedicine Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nano-biomedicine Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDepartment of Studies in Botany, Davangere University, Shivagangothri, Davangere, Karnataka 577507, India; Corresponding author.Lung cancer remains a leading cause of cancer-related mortality, necessitating novel therapeutic strategies. Aristolochia bracteolata Lam. has been traditionally studied for its potential in cancer treatment, and this study explores the green synthesis of silver nanoparticles (AgNPs) using its ethyl acetate extract to evaluate their anticancer potential against human lung adenocarcinoma (NCIH-460) cells. AgNPs biosynthesis was confirmed by a color change and characterized by UV-Vis spectroscopy, FTIR, XRD, zeta potential FE-SEM, and TEM. The UV-Vis spectrum exhibited a characteristic absorption peak at 450 nm, confirming nanoparticle formation. XRD analysis indicated the crystalline nature of AgNPs, while FTIR spectra identified key functional groups involved in nanoparticle stabilization, including O-H, C-H, CC, and C-O bonds. FE-SEM and TEM images showed polydispersed, mostly spherical AgNPs ranging from 4 to 171 nm, with an average TEM-derived size of 10 nm. Zeta potential analysis showed a charge of −26 mV, suggesting moderate stability in solution. The AgNPs exhibited potent cytotoxicity against NCIH-460 cells (IC₅₀ = 13.44 µg mL−1) while sparing normal L929 cells (IC₅₀ = 196.05 µg mL−1). Annexin V/PI and TUNEL assays confirmed apoptosis induction, while cell cycle analysis demonstrated G0/G1 phase arrest, supported by caspase-3 upregulation. These findings suggest biosynthesized AgNPs as promising anticancer agents, capable of inducing apoptosis and disrupting cancer cell proliferation, making them potential candidates for lung cancer therapy.http://www.sciencedirect.com/science/article/pii/S2949829525000439Aristolochia bracteolataSilver nanoparticlesCharacterizationAnticancer activityApoptosis |
| spellingShingle | H.S. Anil Kumar Santosh Mallikarjun Bhavi Sapam Riches Singh Bothe Thokchom Ramesh Babu Yarajarla D. Kotresha Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells Next Nanotechnology Aristolochia bracteolata Silver nanoparticles Characterization Anticancer activity Apoptosis |
| title | Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells |
| title_full | Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells |
| title_fullStr | Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells |
| title_full_unstemmed | Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells |
| title_short | Biosynthesis of silver nanoparticles using Aristolochia bracteolata Lam. ethyl acetate extract: Characterization and In Vitro anticancer activity against lung adenocarcinoma cells |
| title_sort | biosynthesis of silver nanoparticles using aristolochia bracteolata lam ethyl acetate extract characterization and in vitro anticancer activity against lung adenocarcinoma cells |
| topic | Aristolochia bracteolata Silver nanoparticles Characterization Anticancer activity Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2949829525000439 |
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