Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value
Nefang is a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), used for the treatment of malaria. Compounds with antioxidant activity are believed to modulate plasmodial infection. An...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2013/678458 |
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| author | Protus Arrey Tarkang Achille Parfait Nwachiban Atchan Jules-Roger Kuiate Faith Apoelot Okalebo Anastasia Nkatha Guantai Gabriel Agbor Agbor |
| author_facet | Protus Arrey Tarkang Achille Parfait Nwachiban Atchan Jules-Roger Kuiate Faith Apoelot Okalebo Anastasia Nkatha Guantai Gabriel Agbor Agbor |
| author_sort | Protus Arrey Tarkang |
| collection | DOAJ |
| description | Nefang is a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), used for the treatment of malaria. Compounds with antioxidant activity are believed to modulate plasmodial infection. Antioxidant activity of the constituent aqueous plants extracts, in vitro, was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) methods and, in vivo, Nefang (100 and 500 mg kg−1) activity was evaluated in carbon tetrachloride-induced oxidative stressed Wistar rats. Superoxide dismutase, catalase activities, and lipid peroxidation by the malondialdehyde and total proteins assays were carried out. P. guajava, M. indica leaf, and bark extracts had the highest antioxidant properties in all three assays, with no statistically significant difference. Rats treated with the carbon tetrachloride had a statistically significant decrease in levels of triglycerides, superoxide dismutase, and catalase (P<0.05) and increase in malondialdehyde activity, total protein levels, and liver and renal function markers, whereas rats treated with Nefang showed increased levels in the former and dose-dependent decrease towards normal levels in the later. These results reveal the constituent plants of Nefang that contribute to its in vivo antioxidant potential. This activity is a good indication of the therapeutic potential of Nefang. |
| format | Article |
| id | doaj-art-421b8f9592aa4f40a1eba778502aae17 |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-421b8f9592aa4f40a1eba778502aae172025-08-20T03:55:43ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422013-01-01201310.1155/2013/678458678458Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic ValueProtus Arrey Tarkang0Achille Parfait Nwachiban Atchan1Jules-Roger Kuiate2Faith Apoelot Okalebo3Anastasia Nkatha Guantai4Gabriel Agbor Agbor5Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, CameroonDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, CameroonDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, CameroonDepartment of Pharmacology and Pharmacognosy, University of Nairobi, P.O. Box 19676-00202, Nairobi, KenyaDepartment of Pharmacology and Pharmacognosy, University of Nairobi, P.O. Box 19676-00202, Nairobi, KenyaInstitute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, CameroonNefang is a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), used for the treatment of malaria. Compounds with antioxidant activity are believed to modulate plasmodial infection. Antioxidant activity of the constituent aqueous plants extracts, in vitro, was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) methods and, in vivo, Nefang (100 and 500 mg kg−1) activity was evaluated in carbon tetrachloride-induced oxidative stressed Wistar rats. Superoxide dismutase, catalase activities, and lipid peroxidation by the malondialdehyde and total proteins assays were carried out. P. guajava, M. indica leaf, and bark extracts had the highest antioxidant properties in all three assays, with no statistically significant difference. Rats treated with the carbon tetrachloride had a statistically significant decrease in levels of triglycerides, superoxide dismutase, and catalase (P<0.05) and increase in malondialdehyde activity, total protein levels, and liver and renal function markers, whereas rats treated with Nefang showed increased levels in the former and dose-dependent decrease towards normal levels in the later. These results reveal the constituent plants of Nefang that contribute to its in vivo antioxidant potential. This activity is a good indication of the therapeutic potential of Nefang.http://dx.doi.org/10.1155/2013/678458 |
| spellingShingle | Protus Arrey Tarkang Achille Parfait Nwachiban Atchan Jules-Roger Kuiate Faith Apoelot Okalebo Anastasia Nkatha Guantai Gabriel Agbor Agbor Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value Advances in Pharmacological Sciences |
| title | Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value |
| title_full | Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value |
| title_fullStr | Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value |
| title_full_unstemmed | Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value |
| title_short | Antioxidant Potential of a Polyherbal Antimalarial as an Indicator of Its Therapeutic Value |
| title_sort | antioxidant potential of a polyherbal antimalarial as an indicator of its therapeutic value |
| url | http://dx.doi.org/10.1155/2013/678458 |
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