Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for c...
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Pontificia Universidad Javeriana
2019-11-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/22416 |
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| author | Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos |
| author_facet | Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos |
| author_sort | Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos |
| collection | DOAJ |
| description | In tropical countries, the control of the mosquito Aedes aegypti is a public health priority
due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized
as abundant sources of bioactive compounds, and they constitute a potential source of
insecticides useful for controlling mosquito populations and preventing epidemic outbreaks.
We collected 30 benthic cyanobacterial mats in Providencia and Rosario islands (in the
Colombian Caribbean) belonging to the genera Phormidium, Symploca, Oscillatoria, Lyngbya,
Pseudoanabaena, Leptolyngbya, Moorea, and Dapis. Fractions of organic extracts from the most
abundant environmental samples were evaluated in three bioassays, assessing (i) larvicidal
activity against A. aegypti, (ii) toxicity against the brine shrimp (Artemia salina) nauplii, and
(iii) acetylcholinesterase inhibition. Non-polar fractions exhibited larvicidal activity. The
polar fraction from one Dapis pleuosa extract showed larvicidal activity without being toxic
against A. salina nauplii. Extracts from Moorea producens exhibited the greatest toxicity against
A. aegypti larvae and A. salina nauplii. From 23 cultured cyanobacterial samples, only five
grew under laboratory conditions and produced enough biomass to yield organic extracts. Of
these, three extracts showed strong larvicidal activity, but only the extract from Phormidium
tenue showed reduced toxicity against A. salina nauplii. We detected variation among the
chemical profiles and larvicidal activity of cyanobacterial consortia depending on sites and
dates of collection. Our findings suggest that despite variation in chemical profiles, extracts
of marine benthic cyanobacteria can be further developed as effective control agents against
insect vectors, in their larval stages. The culture of marine benthic cyanobacteria needs
to be further explored to provide enough biomass leading to the identification of bioactive
compounds with public health applications. |
| format | Article |
| id | doaj-art-421b32452207436bbfbb6d1ecd3d13a4 |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-421b32452207436bbfbb6d1ecd3d13a42025-08-20T02:16:30ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522019-11-0124346549610.11144/Javeriana.SC24-3.eaccEnvironmental and cultured cyanobacteria as sources of Aedes aegypti larvicidesFarja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos0Universidad Nacional de Colombia, Sede Bogotá, Facultad de Ciencias, Departamento de Química, Address: Carrera 30 # 45-03 edificio 451, Bogotá, Colombia, Postal Code: 111321, Universidad Jorge Tadeo Lozano, Facultad de Ciencias Naturales e Ingeniería, Departamento de Ciencias Biológicas y Ambientales, Address: Carrera 4 # 22-61, Bogotá, Colombia, Postal Code: 110311.In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for controlling mosquito populations and preventing epidemic outbreaks. We collected 30 benthic cyanobacterial mats in Providencia and Rosario islands (in the Colombian Caribbean) belonging to the genera Phormidium, Symploca, Oscillatoria, Lyngbya, Pseudoanabaena, Leptolyngbya, Moorea, and Dapis. Fractions of organic extracts from the most abundant environmental samples were evaluated in three bioassays, assessing (i) larvicidal activity against A. aegypti, (ii) toxicity against the brine shrimp (Artemia salina) nauplii, and (iii) acetylcholinesterase inhibition. Non-polar fractions exhibited larvicidal activity. The polar fraction from one Dapis pleuosa extract showed larvicidal activity without being toxic against A. salina nauplii. Extracts from Moorea producens exhibited the greatest toxicity against A. aegypti larvae and A. salina nauplii. From 23 cultured cyanobacterial samples, only five grew under laboratory conditions and produced enough biomass to yield organic extracts. Of these, three extracts showed strong larvicidal activity, but only the extract from Phormidium tenue showed reduced toxicity against A. salina nauplii. We detected variation among the chemical profiles and larvicidal activity of cyanobacterial consortia depending on sites and dates of collection. Our findings suggest that despite variation in chemical profiles, extracts of marine benthic cyanobacteria can be further developed as effective control agents against insect vectors, in their larval stages. The culture of marine benthic cyanobacteria needs to be further explored to provide enough biomass leading to the identification of bioactive compounds with public health applications.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/22416cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors |
| spellingShingle | Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides Universitas Scientiarum cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors |
| title | Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides |
| title_full | Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides |
| title_fullStr | Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides |
| title_full_unstemmed | Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides |
| title_short | Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides |
| title_sort | environmental and cultured cyanobacteria as sources of aedes aegypti larvicides |
| topic | cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/22416 |
| work_keys_str_mv | AT farjaiayalalaurambecerrajairoquintanalinambayonafreddyaramosmonicapuyanafredyduqueleonardocastellanos environmentalandculturedcyanobacteriaassourcesofaedesaegyptilarvicides |