Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37
The isolation and characterization of bioactive metabolites from <i>Streptomyces</i> species continue to represent a vital area of research, given their potential in natural product drug discovery. In this study, we characterize a new siderophore called legonoxamine I, together with a kn...
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MDPI AG
2025-01-01
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| Series: | Molecules |
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| author | Fleurdeliz Maglangit Qing Fang Jioji N. Tabudravu Kwaku Kyeremeh Marcel Jaspars Hai Deng |
| author_facet | Fleurdeliz Maglangit Qing Fang Jioji N. Tabudravu Kwaku Kyeremeh Marcel Jaspars Hai Deng |
| author_sort | Fleurdeliz Maglangit |
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| description | The isolation and characterization of bioactive metabolites from <i>Streptomyces</i> species continue to represent a vital area of research, given their potential in natural product drug discovery. In this study, we characterize a new siderophore called legonoxamine I, together with a known compound, streptimidone, from the talented soil bacterium <i>Streptomyces</i> sp. MA37, using chromatographic techniques and spectroscopic analysis. Legonoxamine I is a new holo-siderophore, which is likely to be a derailed product from the biosynthetic pathway of legonoxamine A. We also demonstrate that legonoxamine A possesses potent anticancer activity (IC<sub>50</sub> = 2.2 µM), exhibiting a remarkable ~30-fold increase in potency against MCF-7 ATCC HTB-22 breast cancer cells compared to desferrioxamine B, a structural analogue of legonoxamine A (IC<sub>50</sub> = 61.1 µM). Comparing the structural difference between legonoxamine A and desferrioxamine B, it is deduced that the phenylacetyl moiety in legonoxamine A may have contributed significantly to its enhanced potency. Our findings contribute to the growing library of <i>Streptomyces</i>-derived metabolites and underscore the genus’ potential as a promising source of lead compounds. |
| format | Article |
| id | doaj-art-420b07979bf04018b14a479c125a52df |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-420b07979bf04018b14a479c125a52df2025-01-24T13:43:31ZengMDPI AGMolecules1420-30492025-01-0130230610.3390/molecules30020306Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37Fleurdeliz Maglangit0Qing Fang1Jioji N. Tabudravu2Kwaku Kyeremeh3Marcel Jaspars4Hai Deng5Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu, Lahug, Cebu City 6000, PhilippinesMarine Biodiscovery Centre, Department of Chemistry, School of Natural and Computing Sciences, University of Aberdeen, Old Aberdeen AB24 3UE, UKSchool of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UKMarine and Plant Research Laboratory of Ghana, Department of Chemistry, University of Ghana, Legon-Accra P.O. Box LG56, GhanaMarine Biodiscovery Centre, Department of Chemistry, School of Natural and Computing Sciences, University of Aberdeen, Old Aberdeen AB24 3UE, UKMarine Biodiscovery Centre, Department of Chemistry, School of Natural and Computing Sciences, University of Aberdeen, Old Aberdeen AB24 3UE, UKThe isolation and characterization of bioactive metabolites from <i>Streptomyces</i> species continue to represent a vital area of research, given their potential in natural product drug discovery. In this study, we characterize a new siderophore called legonoxamine I, together with a known compound, streptimidone, from the talented soil bacterium <i>Streptomyces</i> sp. MA37, using chromatographic techniques and spectroscopic analysis. Legonoxamine I is a new holo-siderophore, which is likely to be a derailed product from the biosynthetic pathway of legonoxamine A. We also demonstrate that legonoxamine A possesses potent anticancer activity (IC<sub>50</sub> = 2.2 µM), exhibiting a remarkable ~30-fold increase in potency against MCF-7 ATCC HTB-22 breast cancer cells compared to desferrioxamine B, a structural analogue of legonoxamine A (IC<sub>50</sub> = 61.1 µM). Comparing the structural difference between legonoxamine A and desferrioxamine B, it is deduced that the phenylacetyl moiety in legonoxamine A may have contributed significantly to its enhanced potency. Our findings contribute to the growing library of <i>Streptomyces</i>-derived metabolites and underscore the genus’ potential as a promising source of lead compounds.https://www.mdpi.com/1420-3049/30/2/306legonoxaminehydroxamatehydroxylamineiron chelators |
| spellingShingle | Fleurdeliz Maglangit Qing Fang Jioji N. Tabudravu Kwaku Kyeremeh Marcel Jaspars Hai Deng Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 Molecules legonoxamine hydroxamate hydroxylamine iron chelators |
| title | Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 |
| title_full | Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 |
| title_fullStr | Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 |
| title_full_unstemmed | Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 |
| title_short | Isolation and Bioactivity of Natural Products from <i>Streptomyces</i> sp. MA37 |
| title_sort | isolation and bioactivity of natural products from i streptomyces i sp ma37 |
| topic | legonoxamine hydroxamate hydroxylamine iron chelators |
| url | https://www.mdpi.com/1420-3049/30/2/306 |
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