CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION
Aim: optimization of the surgical treatment policy with orthotopic liver transplantation (OLT) depending on the results of intraoperative fl owmetry and the state of intrahepatic microcirculatory bloodstream according to immunohistochemical (IHC) study of microspecimens of the donor’s liver.Material...
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2014-12-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/469 |
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| author | D. A. Granov I. I. Tileubergenov S. V. Shapoval D. N. Maystrenko K. M. Pozharissky A. G. Kudaybergenova V. V. Borovik I. O. Rutkin |
| author_facet | D. A. Granov I. I. Tileubergenov S. V. Shapoval D. N. Maystrenko K. M. Pozharissky A. G. Kudaybergenova V. V. Borovik I. O. Rutkin |
| author_sort | D. A. Granov |
| collection | DOAJ |
| description | Aim: optimization of the surgical treatment policy with orthotopic liver transplantation (OLT) depending on the results of intraoperative fl owmetry and the state of intrahepatic microcirculatory bloodstream according to immunohistochemical (IHC) study of microspecimens of the donor’s liver.Materials and methods. 60 patients are included in the study. Group I (n = 30) comprised of patients for whom it was not necessary to perform any additional interventions on the bloodstream in the hepatopancreatobiliary area during OLT. Group II (n = 30) had patients with insuffi cient arterial blood supply for the graft in the intraoperative stage where it was needed to perform additional and/or repeated interventions in the arteries of the hepatopancreatobilliary area. Intraoperative fl owmetry with assessment of the volume blood circulation (VBC) in the hepatic artery (HA) was carried out in the both studied groups. Reference value of VBC was 100 ml/min and higher. Before and after reperfusion in the liver biopsy material we performed immunohistochemical study with the use of endothelial marker CD 31 with subsequent morphometric estimation of the specifi c square of the microvascular bloodstream.Results. In both groups there was no change in the specifi c square in the areas of portal tract and central vein before and after restoring blood fl ow. In the second group, an 8 times increase of the specifi c square of sinusoids was observed after restoring blood fl ow (р < 0,01).Conclusion. Intraoperative fl owmetric control of the blood fl ow allows in due time to perform surgical correction of the graft arterial blood supply during OLT, and it reduces the risk of thrombosis up to 0%. The value of VBC in the hepatic artery (HA) has reliable dependence upon the state of microcirculatory bloodstream of cadaveric donor’s liver after reperfusion. |
| format | Article |
| id | doaj-art-420217c7dddd41f59c52a2c4d4d0c540 |
| institution | Kabale University |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2014-12-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-420217c7dddd41f59c52a2c4d4d0c5402025-08-20T03:44:21ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912014-12-01164333910.15825/1995-1191-2014-4-33-39407CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATIOND. A. Granov0I. I. Tileubergenov1S. V. Shapoval2D. N. Maystrenko3K. M. Pozharissky4A. G. Kudaybergenova5V. V. Borovik6I. O. Rutkin7Department of operative surgery and interventional radiology FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationDepartment of operative surgery and interventional radiology FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationDepartment of operative surgery and interventional radiology FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationDivision of cardio-vascular surgery FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationLaboratory of immunohistochemistry FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationLaboratory of immunohistochemistry FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationDepartment of operative surgery and interventional radiology FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationDepartment of operative surgery and interventional radiology FSBI «Russian research center for radiology and surgical technologies» the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russian FederationAim: optimization of the surgical treatment policy with orthotopic liver transplantation (OLT) depending on the results of intraoperative fl owmetry and the state of intrahepatic microcirculatory bloodstream according to immunohistochemical (IHC) study of microspecimens of the donor’s liver.Materials and methods. 60 patients are included in the study. Group I (n = 30) comprised of patients for whom it was not necessary to perform any additional interventions on the bloodstream in the hepatopancreatobiliary area during OLT. Group II (n = 30) had patients with insuffi cient arterial blood supply for the graft in the intraoperative stage where it was needed to perform additional and/or repeated interventions in the arteries of the hepatopancreatobilliary area. Intraoperative fl owmetry with assessment of the volume blood circulation (VBC) in the hepatic artery (HA) was carried out in the both studied groups. Reference value of VBC was 100 ml/min and higher. Before and after reperfusion in the liver biopsy material we performed immunohistochemical study with the use of endothelial marker CD 31 with subsequent morphometric estimation of the specifi c square of the microvascular bloodstream.Results. In both groups there was no change in the specifi c square in the areas of portal tract and central vein before and after restoring blood fl ow. In the second group, an 8 times increase of the specifi c square of sinusoids was observed after restoring blood fl ow (р < 0,01).Conclusion. Intraoperative fl owmetric control of the blood fl ow allows in due time to perform surgical correction of the graft arterial blood supply during OLT, and it reduces the risk of thrombosis up to 0%. The value of VBC in the hepatic artery (HA) has reliable dependence upon the state of microcirculatory bloodstream of cadaveric donor’s liver after reperfusion.https://journal.transpl.ru/vtio/article/view/469orthotopic liver transplantationthrombosis of the hepatic arteryvolume blood circulationspecifi c square of sinusoidsintraoperative fl owmetryimmunohistochemical studyliver tissuemorphometry |
| spellingShingle | D. A. Granov I. I. Tileubergenov S. V. Shapoval D. N. Maystrenko K. M. Pozharissky A. G. Kudaybergenova V. V. Borovik I. O. Rutkin CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION Вестник трансплантологии и искусственных органов orthotopic liver transplantation thrombosis of the hepatic artery volume blood circulation specifi c square of sinusoids intraoperative fl owmetry immunohistochemical study liver tissue morphometry |
| title | CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION |
| title_full | CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION |
| title_fullStr | CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION |
| title_full_unstemmed | CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION |
| title_short | CORRELATION OF VOLUME BLOOD CIRCULATION IN THE HEPATIC ARTERY AND THE STATE OF MICROCIRCULATORY BLOODSTREAM OF THE TRANSPLANTED LIVER AFTER ITS REVASCULIZATION |
| title_sort | correlation of volume blood circulation in the hepatic artery and the state of microcirculatory bloodstream of the transplanted liver after its revasculization |
| topic | orthotopic liver transplantation thrombosis of the hepatic artery volume blood circulation specifi c square of sinusoids intraoperative fl owmetry immunohistochemical study liver tissue morphometry |
| url | https://journal.transpl.ru/vtio/article/view/469 |
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