Combining benzalkonium chloride addition with filtration to inhibit dissolved inorganic carbon alteration during the preservation of water sample in radiocarbon analysis
<p>Benzalkonium chloride (BAC) addition has shown great promise as a disinfectant for measuring <span class="inline-formula"><i>δ</i><sup>13</sup>C</span> and <span class="inline-formula"><sup>14</sup>C</span> of d...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2025-07-01
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| Series: | Ocean Science |
| Online Access: | https://os.copernicus.org/articles/21/1395/2025/os-21-1395-2025.pdf |
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| Summary: | <p>Benzalkonium chloride (BAC) addition has shown great promise as a disinfectant for measuring <span class="inline-formula"><i>δ</i><sup>13</sup>C</span> and <span class="inline-formula"><sup>14</sup>C</span> of dissolved inorganic carbon (DIC) in freshwater samples. However, it was reported that the effectiveness of BAC to prevent DIC change was reduced for the use of seawater samples. The present study aims to evaluate the effectiveness of adding BAC as a disinfectant in carbon isotopic analyses of DIC in water samples. We compared the efficacy of BAC addition, filtration (0.22 <span class="inline-formula">µm</span> polytetrafluoroethylene (PTFE) or 0.2–0.45 <span class="inline-formula">µm</span> polyether sulfone (PES) filters), and a combination of BAC addition and filtration in preventing DIC alterations caused by biological activity using the freshwater (salinity <span class="inline-formula"><0.05</span>) and the brackish water (salinity <span class="inline-formula">∼20</span>) samples. The freshwater sample treated with BAC showed no alteration of DIC. In contrast, for the seawater sample, BAC addition alone did not prevent changes in DIC, but the combined treatment was effective. The <span class="inline-formula"><sup>14</sup>C</span> concentration of samples treated with both BAC addition and filtration exhibited minimal changes, ranging from 0.2–0.4 percent modern carbon (pMC) over 41 weeks despite the addition of sugar included to increase DIC changes several-fold. Although the complete elimination of biological effects may be challenging with the combined method, the observed changes remained within practical limits. Concerns about <span class="inline-formula">CO<sub>2</sub></span> contamination during sample filtration were also addressed and found to be negligible. These results suggest that combining filtration and BAC addition is an effective method for suppressing biological DIC alterations in <span class="inline-formula"><sup>14</sup>C</span> analysis, even in seawater samples.</p> |
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| ISSN: | 1812-0784 1812-0792 |