Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination
Abstract Cytospora species are destructive canker and dieback pathogens of woody hosts in natural and agroecosystems around the world. In this genus, molecular identification has been limited due to the paucity of multi-locus sequence typing studies and the lack of sequence data from type specimens...
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BMC
2018-12-01
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| Series: | IMA Fungus |
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| Online Access: | http://link.springer.com/article/10.5598/imafungus.2018.09.02.07 |
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| author | Daniel P. Lawrence Leslie A. Holland Mohamed T. Nouri Renaud Travadon Ara Abramians Themis J. Michailides Florent P. Trouillas |
| author_facet | Daniel P. Lawrence Leslie A. Holland Mohamed T. Nouri Renaud Travadon Ara Abramians Themis J. Michailides Florent P. Trouillas |
| author_sort | Daniel P. Lawrence |
| collection | DOAJ |
| description | Abstract Cytospora species are destructive canker and dieback pathogens of woody hosts in natural and agroecosystems around the world. In this genus, molecular identification has been limited due to the paucity of multi-locus sequence typing studies and the lack of sequence data from type specimens in public repositories, stalling robust phylogenetic reconstructions. In most cases a morphological species concept could not be applied due to the plasticity of characters and significant overlap of morphological features such as spore dimensions and fruiting body characters. In this study, we employed a molecular phylogenetic framework with the inclusion of four nuclear loci (ITS, translation elongation factor 1-alpha, actin, and beta-tubulin) to unveil the biodiversity and taxonomy of this understudied important genus of plant pathogens. Phylogenetic inferences based on 150 Californian isolates revealed 15 Cytospora species associated with branch and twig cankers and dieback of almond, apricot, cherry, cottonwood, olive, peach, pistachio, plum, pomegranate, and walnut trees in California. Of the 15 species recovered in this study, 10 are newly described and typified, in addition to one new combination. The pathogenic status of the newly described Cytospora species requires further investigation as most species were associated with severe dieback and decline of diverse and economically important fruit and nut crops in California. |
| format | Article |
| id | doaj-art-41fd2327250d4f2c9236a45de59ccd3d |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-41fd2327250d4f2c9236a45de59ccd3d2025-02-02T20:53:12ZengBMCIMA Fungus2210-63592018-12-019233336910.5598/imafungus.2018.09.02.07Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combinationDaniel P. Lawrence0Leslie A. Holland1Mohamed T. Nouri2Renaud Travadon3Ara Abramians4Themis J. Michailides5Florent P. Trouillas6Department of Plant Pathology, University of California, DavisDepartment of Plant Pathology, University of California, DavisDepartment of Plant Pathology, University of CaliforniaDepartment of Plant Pathology, University of California, DavisDepartment of Plant Pathology, University of California, DavisDepartment of Plant Pathology, University of CaliforniaDepartment of Plant Pathology, University of CaliforniaAbstract Cytospora species are destructive canker and dieback pathogens of woody hosts in natural and agroecosystems around the world. In this genus, molecular identification has been limited due to the paucity of multi-locus sequence typing studies and the lack of sequence data from type specimens in public repositories, stalling robust phylogenetic reconstructions. In most cases a morphological species concept could not be applied due to the plasticity of characters and significant overlap of morphological features such as spore dimensions and fruiting body characters. In this study, we employed a molecular phylogenetic framework with the inclusion of four nuclear loci (ITS, translation elongation factor 1-alpha, actin, and beta-tubulin) to unveil the biodiversity and taxonomy of this understudied important genus of plant pathogens. Phylogenetic inferences based on 150 Californian isolates revealed 15 Cytospora species associated with branch and twig cankers and dieback of almond, apricot, cherry, cottonwood, olive, peach, pistachio, plum, pomegranate, and walnut trees in California. Of the 15 species recovered in this study, 10 are newly described and typified, in addition to one new combination. The pathogenic status of the newly described Cytospora species requires further investigation as most species were associated with severe dieback and decline of diverse and economically important fruit and nut crops in California.http://link.springer.com/article/10.5598/imafungus.2018.09.02.07CytosporaceaeCytospora cankerDiaporthalesmultigene phylogenynew taxataxonomy |
| spellingShingle | Daniel P. Lawrence Leslie A. Holland Mohamed T. Nouri Renaud Travadon Ara Abramians Themis J. Michailides Florent P. Trouillas Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination IMA Fungus Cytosporaceae Cytospora canker Diaporthales multigene phylogeny new taxa taxonomy |
| title | Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination |
| title_full | Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination |
| title_fullStr | Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination |
| title_full_unstemmed | Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination |
| title_short | Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination |
| title_sort | molecular phylogeny of cytospora species associated with canker diseases of fruit and nut crops in california with the descriptions of ten new species and one new combination |
| topic | Cytosporaceae Cytospora canker Diaporthales multigene phylogeny new taxa taxonomy |
| url | http://link.springer.com/article/10.5598/imafungus.2018.09.02.07 |
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