Single-cell states in the estrogen response of breast cancer cell lines.
Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for t...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088485&type=printable |
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| author | Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi |
| author_facet | Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi |
| author_sort | Francesco Paolo Casale |
| collection | DOAJ |
| description | Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified. |
| format | Article |
| id | doaj-art-41f927f14c2b4216af613dacc620646e |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-41f927f14c2b4216af613dacc620646e2025-08-20T03:11:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8848510.1371/journal.pone.0088485Single-cell states in the estrogen response of breast cancer cell lines.Francesco Paolo CasaleGiorgio GiuratoGiovanni NassaJonathan W ArmondChris J OatesDavide CoráAndrea GambaSach MukherjeeAlessandro WeiszMario NicodemiEstrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088485&type=printable |
| spellingShingle | Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi Single-cell states in the estrogen response of breast cancer cell lines. PLoS ONE |
| title | Single-cell states in the estrogen response of breast cancer cell lines. |
| title_full | Single-cell states in the estrogen response of breast cancer cell lines. |
| title_fullStr | Single-cell states in the estrogen response of breast cancer cell lines. |
| title_full_unstemmed | Single-cell states in the estrogen response of breast cancer cell lines. |
| title_short | Single-cell states in the estrogen response of breast cancer cell lines. |
| title_sort | single cell states in the estrogen response of breast cancer cell lines |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088485&type=printable |
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