Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole
In this paper, peanut protein (PP) was used as the sole raw material for the preparation of fluorescent carbon dots (PP-CDs) by hydrothermal method. The PP-CDs exhibit good dispersibility, spherical-like shapes, and uniform sizes; the average particle size of the PP-CDs was 3.18 ± 0.17 nm. The Fouri...
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MDPI AG
2025-04-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/13/4/151 |
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| author | Junyan Liao Zhixiong Hu Weinong Zhang Yanpeng Zhang Jiangrong Xiao Shenglan Lei |
| author_facet | Junyan Liao Zhixiong Hu Weinong Zhang Yanpeng Zhang Jiangrong Xiao Shenglan Lei |
| author_sort | Junyan Liao |
| collection | DOAJ |
| description | In this paper, peanut protein (PP) was used as the sole raw material for the preparation of fluorescent carbon dots (PP-CDs) by hydrothermal method. The PP-CDs exhibit good dispersibility, spherical-like shapes, and uniform sizes; the average particle size of the PP-CDs was 3.18 ± 0.17 nm. The Fourier transform infrared spectroscopy (FTIR) results show that the surface of PP-CDs is rich in hydrophilic groups such as hydroxyl, carboxyl and amide groups. The PP-CDs exhibit good fluorescence emission properties and excitation wavelength dependence, with the optimal excitation wavelength and emission wavelength at 348 nm and 452 nm, respectively. According to the fluorescence quenching effect of metronidazole (MTZ) and tinidazole (TDZ) on PP-CDs, a highly linear fluorescence sensor was established, with a concentration range of 0.10–60.0 µM, and the detection limits of MTZ and TDZ are 32.0 nM and 48.0 nM, respectively. The result of CCK-8 test and imaging of HepG-2 cells and onion epidermal cells reveal that PP-CDs have good membrane permeability, biocompatibility and imaging ability. |
| format | Article |
| id | doaj-art-41ec4bdd6db846d785758a73c2dbe972 |
| institution | DOAJ |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Chemosensors |
| spelling | doaj-art-41ec4bdd6db846d785758a73c2dbe9722025-08-20T03:13:46ZengMDPI AGChemosensors2227-90402025-04-0113415110.3390/chemosensors13040151Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of MetronidazoleJunyan Liao0Zhixiong Hu1Weinong Zhang2Yanpeng Zhang3Jiangrong Xiao4Shenglan Lei5College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaIn this paper, peanut protein (PP) was used as the sole raw material for the preparation of fluorescent carbon dots (PP-CDs) by hydrothermal method. The PP-CDs exhibit good dispersibility, spherical-like shapes, and uniform sizes; the average particle size of the PP-CDs was 3.18 ± 0.17 nm. The Fourier transform infrared spectroscopy (FTIR) results show that the surface of PP-CDs is rich in hydrophilic groups such as hydroxyl, carboxyl and amide groups. The PP-CDs exhibit good fluorescence emission properties and excitation wavelength dependence, with the optimal excitation wavelength and emission wavelength at 348 nm and 452 nm, respectively. According to the fluorescence quenching effect of metronidazole (MTZ) and tinidazole (TDZ) on PP-CDs, a highly linear fluorescence sensor was established, with a concentration range of 0.10–60.0 µM, and the detection limits of MTZ and TDZ are 32.0 nM and 48.0 nM, respectively. The result of CCK-8 test and imaging of HepG-2 cells and onion epidermal cells reveal that PP-CDs have good membrane permeability, biocompatibility and imaging ability.https://www.mdpi.com/2227-9040/13/4/151peanut proteincarbon dotscell imagingmetronidazoletinidazolefluorescence sensing |
| spellingShingle | Junyan Liao Zhixiong Hu Weinong Zhang Yanpeng Zhang Jiangrong Xiao Shenglan Lei Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole Chemosensors peanut protein carbon dots cell imaging metronidazole tinidazole fluorescence sensing |
| title | Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole |
| title_full | Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole |
| title_fullStr | Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole |
| title_full_unstemmed | Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole |
| title_short | Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole |
| title_sort | characteristics of peanut protein derived carbon dots and their application in cell imaging and sensing of metronidazole |
| topic | peanut protein carbon dots cell imaging metronidazole tinidazole fluorescence sensing |
| url | https://www.mdpi.com/2227-9040/13/4/151 |
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