Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis
Methanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii, a methanol-assimilating yeast, is a useful host for the chemical production from methanol. A previous study successfully constructed a metabolically engineered K. phaffii GS115/S8/Z3 strain capable o...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Metabolic Engineering Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030125000069 |
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| author | Yoshifumi Inoue Kaito Nakamura Ryosuke Yamada Takuya Matsumoto Hiroyasu Ogino |
| author_facet | Yoshifumi Inoue Kaito Nakamura Ryosuke Yamada Takuya Matsumoto Hiroyasu Ogino |
| author_sort | Yoshifumi Inoue |
| collection | DOAJ |
| description | Methanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii, a methanol-assimilating yeast, is a useful host for the chemical production from methanol. A previous study successfully constructed a metabolically engineered K. phaffii GS115/S8/Z3 strain capable of producing D-lactic acid from methanol. In this study, we aimed to develop a strain with improved D-lactic acid production by applying ultra-violet mutagenesis to the D-lactic acid-producing strain, GS115/S8/Z3. The resulting mutant strain DLac_Mut2_221 produced 5.38 g/L of D-lactic acid from methanol, a 1.52-fold increase compared to the parent strain GS115/S8/Z3. The transcriptome analysis of the mutant DLac_Mut2_221 identified 158 differentially expressed genes, providing insights into key mechanisms contributing to enhanced D-lactic acid production. Metabolic engineering strategies for K. phaffii based on the knowledge gained from this study will contribute to improving the productivity of various useful chemicals from methanol. |
| format | Article |
| id | doaj-art-41e824d04a6246b8b4c5b642cbfee6b0 |
| institution | DOAJ |
| issn | 2214-0301 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj-art-41e824d04a6246b8b4c5b642cbfee6b02025-08-20T03:21:51ZengElsevierMetabolic Engineering Communications2214-03012025-06-0120e0026210.1016/j.mec.2025.e00262Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesisYoshifumi Inoue0Kaito Nakamura1Ryosuke Yamada2Takuya Matsumoto3Hiroyasu Ogino4Osaka Metropolitan University, Department of Chemical Engineering, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, JapanOsaka Metropolitan University, Department of Chemical Engineering, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, JapanCorresponding author.; Osaka Metropolitan University, Department of Chemical Engineering, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, JapanOsaka Metropolitan University, Department of Chemical Engineering, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, JapanOsaka Metropolitan University, Department of Chemical Engineering, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, JapanMethanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii, a methanol-assimilating yeast, is a useful host for the chemical production from methanol. A previous study successfully constructed a metabolically engineered K. phaffii GS115/S8/Z3 strain capable of producing D-lactic acid from methanol. In this study, we aimed to develop a strain with improved D-lactic acid production by applying ultra-violet mutagenesis to the D-lactic acid-producing strain, GS115/S8/Z3. The resulting mutant strain DLac_Mut2_221 produced 5.38 g/L of D-lactic acid from methanol, a 1.52-fold increase compared to the parent strain GS115/S8/Z3. The transcriptome analysis of the mutant DLac_Mut2_221 identified 158 differentially expressed genes, providing insights into key mechanisms contributing to enhanced D-lactic acid production. Metabolic engineering strategies for K. phaffii based on the knowledge gained from this study will contribute to improving the productivity of various useful chemicals from methanol.http://www.sciencedirect.com/science/article/pii/S2214030125000069D-lactic acidKomagataella phaffiiMethanolUltra-violet mutagenesisTranscriptome analysisYeast |
| spellingShingle | Yoshifumi Inoue Kaito Nakamura Ryosuke Yamada Takuya Matsumoto Hiroyasu Ogino Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis Metabolic Engineering Communications D-lactic acid Komagataella phaffii Methanol Ultra-violet mutagenesis Transcriptome analysis Yeast |
| title | Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis |
| title_full | Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis |
| title_fullStr | Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis |
| title_full_unstemmed | Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis |
| title_short | Improvement of D-lactic acid production from methanol by metabolically engineered Komagataella phaffii via ultra-violet mutagenesis |
| title_sort | improvement of d lactic acid production from methanol by metabolically engineered komagataella phaffii via ultra violet mutagenesis |
| topic | D-lactic acid Komagataella phaffii Methanol Ultra-violet mutagenesis Transcriptome analysis Yeast |
| url | http://www.sciencedirect.com/science/article/pii/S2214030125000069 |
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