Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study
Objective: Compare the remineralisation potential of four enamel remineralising agents on artificially demineralized enamel surface using Scanning electron microscopy- Energy dispersive x-ray. Materials and methods: 75 extracted maxillary and mandibular premolars coated with acid-resistant nail varn...
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Elsevier
2025-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000089 |
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| author | R. Raj |
| author_facet | R. Raj |
| author_sort | R. Raj |
| collection | DOAJ |
| description | Objective: Compare the remineralisation potential of four enamel remineralising agents on artificially demineralized enamel surface using Scanning electron microscopy- Energy dispersive x-ray. Materials and methods: 75 extracted maxillary and mandibular premolars coated with acid-resistant nail varnish, were stored in demineralising solution for 96 h to produce artificial caries lesions. The samples were divided into 5 groups (n = 15), Control (Demineralized- No treatment), Group I samples were treated with casein phosphoprotein-amorphous calcium phosphate (CPP-ACP), Group II with self-assembling peptide (SAP-14), Group III with tri-calcium phosphate (f-TCP) and Group IV with Bioactive glass (BAG), respectively. The pH cycling model was followed for 21 days. The samples were analysed via SEM-EDX (Carl Zeiss, Germany; Model: Merlin Compact) for qualitative assessment and quantitative analysis of calcium and phosphorous. The data were analysed for multiple group comparison using IBM SPSS version 20 with one-way ANOVA followed by a paired t-test. Results: Calcium/Phosphorous ratio of all experimental groups; Group 1(1.92 ± .17), Group 2 (1.98 ± .16), Group 3 (1.81 ± .03), Group 4 (1.75 ± .08) was statistically different (p < 0.0005) from Control; while there was no difference between Group I and Group II (p = 0.33). Conclusion: All experimental groups showed comparable remineralising potential. Even though no statistically significant difference is seen between Group I and Group II, after correlating with surface analysis it was concluded that Group II showed the greatest remineralising potential. |
| format | Article |
| id | doaj-art-41d12e826a4c4db5b25505af8a30d043 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-41d12e826a4c4db5b25505af8a30d0432025-02-09T05:00:16ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-01151183187Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro studyR. Raj0Hyderabad, Telangana, India.; Conservative and Endodontics, Adesh Institute of Dental Sciences and Research, Bathinda, IndiaObjective: Compare the remineralisation potential of four enamel remineralising agents on artificially demineralized enamel surface using Scanning electron microscopy- Energy dispersive x-ray. Materials and methods: 75 extracted maxillary and mandibular premolars coated with acid-resistant nail varnish, were stored in demineralising solution for 96 h to produce artificial caries lesions. The samples were divided into 5 groups (n = 15), Control (Demineralized- No treatment), Group I samples were treated with casein phosphoprotein-amorphous calcium phosphate (CPP-ACP), Group II with self-assembling peptide (SAP-14), Group III with tri-calcium phosphate (f-TCP) and Group IV with Bioactive glass (BAG), respectively. The pH cycling model was followed for 21 days. The samples were analysed via SEM-EDX (Carl Zeiss, Germany; Model: Merlin Compact) for qualitative assessment and quantitative analysis of calcium and phosphorous. The data were analysed for multiple group comparison using IBM SPSS version 20 with one-way ANOVA followed by a paired t-test. Results: Calcium/Phosphorous ratio of all experimental groups; Group 1(1.92 ± .17), Group 2 (1.98 ± .16), Group 3 (1.81 ± .03), Group 4 (1.75 ± .08) was statistically different (p < 0.0005) from Control; while there was no difference between Group I and Group II (p = 0.33). Conclusion: All experimental groups showed comparable remineralising potential. Even though no statistically significant difference is seen between Group I and Group II, after correlating with surface analysis it was concluded that Group II showed the greatest remineralising potential.http://www.sciencedirect.com/science/article/pii/S2212426825000089CalciumCPP-ACPSAP 11-4Biomaterial scienceDental cariesDemineralisation |
| spellingShingle | R. Raj Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study Journal of Oral Biology and Craniofacial Research Calcium CPP-ACP SAP 11-4 Biomaterial science Dental caries Demineralisation |
| title | Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study |
| title_full | Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study |
| title_fullStr | Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study |
| title_full_unstemmed | Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study |
| title_short | Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study |
| title_sort | comparative evaluation of remineralizing potential of four enamel remineralising agents using sem edx an in vitro study |
| topic | Calcium CPP-ACP SAP 11-4 Biomaterial science Dental caries Demineralisation |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000089 |
| work_keys_str_mv | AT rraj comparativeevaluationofremineralizingpotentialoffourenamelremineralisingagentsusingsemedxaninvitrostudy |