Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups

Diabetes is the world’s leading cause of renal and premature cardiovascular disease. There are marked differences between groups of patients with different ethnicities in their susceptibility to diabetes and its renal and cardiovascular complications. Novel markers of developing diabetes complicatio...

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Main Authors: Karima Zitouni, Mia Steyn, Joanna Lewis, Frank J. Kelly, Paul Cook, Kenneth A. Earle
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Antioxidants
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Online Access:https://www.mdpi.com/2076-3921/14/7/858
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author Karima Zitouni
Mia Steyn
Joanna Lewis
Frank J. Kelly
Paul Cook
Kenneth A. Earle
author_facet Karima Zitouni
Mia Steyn
Joanna Lewis
Frank J. Kelly
Paul Cook
Kenneth A. Earle
author_sort Karima Zitouni
collection DOAJ
description Diabetes is the world’s leading cause of renal and premature cardiovascular disease. There are marked differences between groups of patients with different ethnicities in their susceptibility to diabetes and its renal and cardiovascular complications. Novel markers of developing diabetes complications are related to disturbances in oxidative metabolism. In this cross-sectional study, we measured the arterial stiffness in patients of differing ethnicities with type 2 diabetes mellitus and assessed the relationship of their ethnicity with systemic markers of oxidative stress. Patients from black, African and Caribbean, and Asian minor ethnic groups were studied, with white patients with T2DM (<i>n</i> = 170) without evidence of cardiovascular disease (CVD). The vascular stiffness was measured by infrared finger-photoplethysmography. The oxidative stress burden was assessed by measuring the urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), activities of plasma glutathione peroxidase (GPx-3), superoxide dismutase (SOD) activities, and concentration of selenium. The vascular stiffness and 8-OHdG were higher in the white than in the Black patients (9.68 m/s vs. 9.26 m/s, <i>p</i> = 0.021 and 292.8 ng/mL vs. 200.9 ng/mL, <i>p</i> = 0.0027, respectively). Meanwhile, the GPx-3 and SOD activities and selenium were lower in the white than in the Black patients (283.3 U/L vs. 440.4 U/L, <i>p</i> < 0.0001; 37.5 U/L vs. 75.6 U/L, <i>p</i> = 0.0007; and 1.14 vs. 1.28 µmol/L, <i>p</i> = 0.0001, respectively). In regression modelling, the 8-OHdG/creatinine ratio was an independent predictor of vascular stiffness in the white patient group (β = 0.23 m/s per unit increase in ln(8-OHdG/creatinine) [95% CI, 0.03 to 0.42]; <i>p</i> = 0.021) but not in the Black patient group (<i>p</i> = 0.29). Increased vascular stiffness, lower endogenous antioxidant defense, and greater levels of oxidative damage were found in patients of white ethnicity, which could contribute to the higher incidence of CVD compared with patients from Black minor ethnic groups with diabetic renal disease.
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spelling doaj-art-41c7fbc17e524e699cfe5d4d1764c2bf2025-08-20T03:55:52ZengMDPI AGAntioxidants2076-39212025-07-0114785810.3390/antiox14070858Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic GroupsKarima Zitouni0Mia Steyn1Joanna Lewis2Frank J. Kelly3Paul Cook4Kenneth A. Earle5Institute of Infection and Immunity, City St George’s, University of London, London SW17 0RE, UKThomas Addison Diabetes Unit, St George’s University Hospitals NHS Foundation Trust, London SW17 0QT, UKPopulation, Policy & Practice Department, University College London, Great Ormond St., Institute of Child Health, London WC1N 1EH, UKEnvironmental Research Group, Faculty of Medicine, School of Public Health, Imperial College London, London W12 0BZ, UKChemical Pathology and Metabolic Medicine, University Hospital Southampton NHS Foundation Trust, Southampton SO16 6YD, UKSt George’s School of Health and Medical Sciences, City St George’s, University of London, London SW17 0RE, UKDiabetes is the world’s leading cause of renal and premature cardiovascular disease. There are marked differences between groups of patients with different ethnicities in their susceptibility to diabetes and its renal and cardiovascular complications. Novel markers of developing diabetes complications are related to disturbances in oxidative metabolism. In this cross-sectional study, we measured the arterial stiffness in patients of differing ethnicities with type 2 diabetes mellitus and assessed the relationship of their ethnicity with systemic markers of oxidative stress. Patients from black, African and Caribbean, and Asian minor ethnic groups were studied, with white patients with T2DM (<i>n</i> = 170) without evidence of cardiovascular disease (CVD). The vascular stiffness was measured by infrared finger-photoplethysmography. The oxidative stress burden was assessed by measuring the urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), activities of plasma glutathione peroxidase (GPx-3), superoxide dismutase (SOD) activities, and concentration of selenium. The vascular stiffness and 8-OHdG were higher in the white than in the Black patients (9.68 m/s vs. 9.26 m/s, <i>p</i> = 0.021 and 292.8 ng/mL vs. 200.9 ng/mL, <i>p</i> = 0.0027, respectively). Meanwhile, the GPx-3 and SOD activities and selenium were lower in the white than in the Black patients (283.3 U/L vs. 440.4 U/L, <i>p</i> < 0.0001; 37.5 U/L vs. 75.6 U/L, <i>p</i> = 0.0007; and 1.14 vs. 1.28 µmol/L, <i>p</i> = 0.0001, respectively). In regression modelling, the 8-OHdG/creatinine ratio was an independent predictor of vascular stiffness in the white patient group (β = 0.23 m/s per unit increase in ln(8-OHdG/creatinine) [95% CI, 0.03 to 0.42]; <i>p</i> = 0.021) but not in the Black patient group (<i>p</i> = 0.29). Increased vascular stiffness, lower endogenous antioxidant defense, and greater levels of oxidative damage were found in patients of white ethnicity, which could contribute to the higher incidence of CVD compared with patients from Black minor ethnic groups with diabetic renal disease.https://www.mdpi.com/2076-3921/14/7/858type 2 diabetes mellituscardio-renal diseasevascular stiffnessoxidative stress8-hydroxy-2′-deoxyguanosineantioxidant enzyme activity
spellingShingle Karima Zitouni
Mia Steyn
Joanna Lewis
Frank J. Kelly
Paul Cook
Kenneth A. Earle
Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
Antioxidants
type 2 diabetes mellitus
cardio-renal disease
vascular stiffness
oxidative stress
8-hydroxy-2′-deoxyguanosine
antioxidant enzyme activity
title Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
title_full Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
title_fullStr Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
title_full_unstemmed Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
title_short Variation in Arterial Stiffness and Markers of Oxidative Stress in Patients with Type 2 Diabetes Mellitus from Different Ethnic Groups
title_sort variation in arterial stiffness and markers of oxidative stress in patients with type 2 diabetes mellitus from different ethnic groups
topic type 2 diabetes mellitus
cardio-renal disease
vascular stiffness
oxidative stress
8-hydroxy-2′-deoxyguanosine
antioxidant enzyme activity
url https://www.mdpi.com/2076-3921/14/7/858
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