Immature seed culture and in vitro propagation of Platycrater arguta
Platycrater arguta, which is one of the Class Ⅱ national endangered species in China, was selected to conduct protective and reproductive study by tissue culture techniques including in vitro culture of immature seeds, in vitro propagation of shoot, colchicine induction, and taking root. When the im...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2019-04-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.03.261 |
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| Summary: | Platycrater arguta, which is one of the Class Ⅱ national endangered species in China, was selected to conduct protective and reproductive study by tissue culture techniques including in vitro culture of immature seeds, in vitro propagation of shoot, colchicine induction, and taking root. When the immature seeds were cultured on the woody plant medium (WPM)+400 mg/L glutamine+500 mg/L casein hydrolysate+2 000 mg/L activated carbon, the germination rate of P. arguta could reach 40% after 35 d. As explants, stem segments of seedlings that germinated from immature seeds were cultured on WPM with different concentrations of 6-benzylaminopurine (6-BA) and 1-naphthylacetic acid (NAA), and MS mediums with different concentrations of colchicine, respectively. The results showed that the WPM with 1 mg/L 6-BA and 0.5 mg/L NAA was best for induction and growth of regeneration buds, and the MS medium with 200 mg/L colchicine could induce mixoploids. Finally, the regeneration buds were transplanted on a WPM supplemented with 0.5 g /mL indole butyric acid (IBA) to induce roots. Some of the plants have been returned to Fengyangshan-Baishanzu National Nature Reserve, Zhejiang. This study provides a new approach to solve the problems such as the poor seed germination rate of mature seed, slow plant growth and regeneration difficulty of P. arguta. |
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| ISSN: | 1008-9209 2097-5155 |