Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis
Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetes-related conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid gly...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000162 |
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| author | Ruyu Shi Xing Li Bing Gao Chunhong Zhang Minhui Li |
| author_facet | Ruyu Shi Xing Li Bing Gao Chunhong Zhang Minhui Li |
| author_sort | Ruyu Shi |
| collection | DOAJ |
| description | Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetes-related conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis. |
| format | Article |
| id | doaj-art-41b5a218ed5a4c7792e7c790567a2cd1 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-41b5a218ed5a4c7792e7c790567a2cd12025-02-03T06:52:50ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112515801589Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosisRuyu Shi0Xing Li1Bing Gao2Chunhong Zhang3Minhui Li4Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China; Pharmaceutical Laboratory, Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, ChinaInner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, ChinaInner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, ChinaInner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China; Inner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs, Baotou Medical College, Baotou 014040, China; Corresponding authors at: Baotou Medical College, Baotou 014040, China.Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China; Pharmaceutical Laboratory, Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs, Baotou Medical College, Baotou 014040, China; Corresponding authors at: Baotou Medical College, Baotou 014040, China.Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetes-related conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis.http://www.sciencedirect.com/science/article/pii/S2213453023000162Diabetes mellitusCymbaria daurica L.Phenylethanoid glycosidesAlloxan-induced cell injury |
| spellingShingle | Ruyu Shi Xing Li Bing Gao Chunhong Zhang Minhui Li Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis Food Science and Human Wellness Diabetes mellitus Cymbaria daurica L. Phenylethanoid glycosides Alloxan-induced cell injury |
| title | Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis |
| title_full | Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis |
| title_fullStr | Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis |
| title_full_unstemmed | Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis |
| title_short | Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis |
| title_sort | phenylethanoid glycosides from traditional mongolian medicine cymbaria daurica alleviate alloxan induced ins 1 cells oxidative stress and apoptosis |
| topic | Diabetes mellitus Cymbaria daurica L. Phenylethanoid glycosides Alloxan-induced cell injury |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000162 |
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