Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application

ABSTRACT This review aims to enable a rapid diagnosis of early‐stage cancer by examining the developments and methods used to collect specific cancer biomarkers, namely, exosomes and circulating tumor cells (CTCs), using microfluidic devices. High throughput, low response time, and accurate control...

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Main Authors: Bahareh Valinezhad Saghezi, Kamran Mansouri, Rahman Hallaj
Format: Article
Language:English
Published: Wiley-VCH 2025-07-01
Series:Nano Select
Subjects:
Online Access:https://doi.org/10.1002/nano.202400108
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author Bahareh Valinezhad Saghezi
Kamran Mansouri
Rahman Hallaj
author_facet Bahareh Valinezhad Saghezi
Kamran Mansouri
Rahman Hallaj
author_sort Bahareh Valinezhad Saghezi
collection DOAJ
description ABSTRACT This review aims to enable a rapid diagnosis of early‐stage cancer by examining the developments and methods used to collect specific cancer biomarkers, namely, exosomes and circulating tumor cells (CTCs), using microfluidic devices. High throughput, low response time, and accurate control of liquid flow are advantages of microfluidic devices compared to traditional technologies, which often suffer from disadvantages of high cost, time‐consuming procedures, and labor‐intensive processes. Microfluidic‐based approaches have the potential to improve early cancer detection and personalized treatment strategies by efficiently collecting and isolating CTCs and extracellular vesicles (EVs), which provide critical information about cancer progression and the response to treatment. An important step toward leveraging these advances and bringing them to clinical use is ensuring portability to the patient's bedside, that is, integrating point‐of‐care (POC) diagnostics with microfluidic technologies. In this article, we first provide a background on cancer and its markers, cancer biology, and liquid biopsies and their benefits in microfluidic systems in diagnosis. Next, we discuss the importance of the basics of microfluidics, techniques based on it in the detection of EVs and CTCs in advancing cancer research and their benefits, as well as integrating systems POC with microfluidic platforms for bedside applications.
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spelling doaj-art-41b250b6a8f84ad1816624e4e3cbbdb12025-08-20T03:50:15ZengWiley-VCHNano Select2688-40112025-07-0167n/an/a10.1002/nano.202400108Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical ApplicationBahareh Valinezhad Saghezi0Kamran Mansouri1Rahman Hallaj2School of Pharmacy Kermanshah University of Medical Sciences Kermanshah IranMedical Biology Research Center Health Technology Institute Kermanshah University of Medical Sciences Kermanshah IranDepartment of Chemistry Faculty of Science University of Kurdistan Sanandaj IranABSTRACT This review aims to enable a rapid diagnosis of early‐stage cancer by examining the developments and methods used to collect specific cancer biomarkers, namely, exosomes and circulating tumor cells (CTCs), using microfluidic devices. High throughput, low response time, and accurate control of liquid flow are advantages of microfluidic devices compared to traditional technologies, which often suffer from disadvantages of high cost, time‐consuming procedures, and labor‐intensive processes. Microfluidic‐based approaches have the potential to improve early cancer detection and personalized treatment strategies by efficiently collecting and isolating CTCs and extracellular vesicles (EVs), which provide critical information about cancer progression and the response to treatment. An important step toward leveraging these advances and bringing them to clinical use is ensuring portability to the patient's bedside, that is, integrating point‐of‐care (POC) diagnostics with microfluidic technologies. In this article, we first provide a background on cancer and its markers, cancer biology, and liquid biopsies and their benefits in microfluidic systems in diagnosis. Next, we discuss the importance of the basics of microfluidics, techniques based on it in the detection of EVs and CTCs in advancing cancer research and their benefits, as well as integrating systems POC with microfluidic platforms for bedside applications.https://doi.org/10.1002/nano.202400108cancer diagnosisCTCexosomeextracellular vesiclesmicrofluidic systemsPOC
spellingShingle Bahareh Valinezhad Saghezi
Kamran Mansouri
Rahman Hallaj
Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
Nano Select
cancer diagnosis
CTC
exosome
extracellular vesicles
microfluidic systems
POC
title Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
title_full Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
title_fullStr Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
title_full_unstemmed Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
title_short Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application
title_sort microfluidic systems in cancer diagnosis advancing exosome and ctc detection from laboratory research to clinical application
topic cancer diagnosis
CTC
exosome
extracellular vesicles
microfluidic systems
POC
url https://doi.org/10.1002/nano.202400108
work_keys_str_mv AT baharehvalinezhadsaghezi microfluidicsystemsincancerdiagnosisadvancingexosomeandctcdetectionfromlaboratoryresearchtoclinicalapplication
AT kamranmansouri microfluidicsystemsincancerdiagnosisadvancingexosomeandctcdetectionfromlaboratoryresearchtoclinicalapplication
AT rahmanhallaj microfluidicsystemsincancerdiagnosisadvancingexosomeandctcdetectionfromlaboratoryresearchtoclinicalapplication