Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser
Introduction. Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications. However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice. W...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/509513 |
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| author | Kathelijne Maaike Groeneveld Marjolein Heeres Loek Petrus Hendrikus Leenen Albert Huisman Leo Koenderman |
| author_facet | Kathelijne Maaike Groeneveld Marjolein Heeres Loek Petrus Hendrikus Leenen Albert Huisman Leo Koenderman |
| author_sort | Kathelijne Maaike Groeneveld |
| collection | DOAJ |
| description | Introduction. Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications. However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice. We tested an approach to overcome these limitations. Material and Methods. Neutrophils of healthy donors were incubated with antibodies commonly used in trauma research: CD11b (MAC-1), L-selectin (CD62L), FcγRIII (CD16), and FcγRII (CD32) in active form (MoPhab A27). Flow cytometric analysis was performed both on a FACSCalibur, a standard flow cytometer, and on a Cell-Dyn Sapphire, a routine haematology analyser. Results. There was a high level of agreement between the two types of analysers, with 41% for FcγRIII, 80% for L-selectin, 98% for CD11b, and even a 100% agreement for active FcγRII. Moreover, analysis on the routine haematology analyser was possible in less than a quarter of the time in comparison to the flow cytometer. Conclusion. Analysis of neutrophil phenotype on the Cell-Dyn Sapphire leads to the same conclusion compared to a standard flow cytometer. The markedly reduced time necessary for analysis and reduced labour intensity constitutes a step forward in implementation of this type of analysis in clinical diagnostics in trauma research. |
| format | Article |
| id | doaj-art-419156be963341eea5216f6b6137c1f5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-419156be963341eea5216f6b6137c1f52025-02-03T01:24:26ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/509513509513Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology AnalyserKathelijne Maaike Groeneveld0Marjolein Heeres1Loek Petrus Hendrikus Leenen2Albert Huisman3Leo Koenderman4Department of Trauma Surgery, University Medical Center Utrecht, 3508 GA Utrecht, The NetherlandsDepartment of Trauma Surgery, University Medical Center Utrecht, 3508 GA Utrecht, The NetherlandsDepartment of Trauma Surgery, University Medical Center Utrecht, 3508 GA Utrecht, The NetherlandsDepartment of Clinical Chemistry and Haematology, University Medical Center Utrecht, 3508 GA Utrecht, The NetherlandsDepartment of Respiratory Medicine, University Medical Center Utrecht, 3508 GA Utrecht, The NetherlandsIntroduction. Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications. However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice. We tested an approach to overcome these limitations. Material and Methods. Neutrophils of healthy donors were incubated with antibodies commonly used in trauma research: CD11b (MAC-1), L-selectin (CD62L), FcγRIII (CD16), and FcγRII (CD32) in active form (MoPhab A27). Flow cytometric analysis was performed both on a FACSCalibur, a standard flow cytometer, and on a Cell-Dyn Sapphire, a routine haematology analyser. Results. There was a high level of agreement between the two types of analysers, with 41% for FcγRIII, 80% for L-selectin, 98% for CD11b, and even a 100% agreement for active FcγRII. Moreover, analysis on the routine haematology analyser was possible in less than a quarter of the time in comparison to the flow cytometer. Conclusion. Analysis of neutrophil phenotype on the Cell-Dyn Sapphire leads to the same conclusion compared to a standard flow cytometer. The markedly reduced time necessary for analysis and reduced labour intensity constitutes a step forward in implementation of this type of analysis in clinical diagnostics in trauma research.http://dx.doi.org/10.1155/2012/509513 |
| spellingShingle | Kathelijne Maaike Groeneveld Marjolein Heeres Loek Petrus Hendrikus Leenen Albert Huisman Leo Koenderman Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser Mediators of Inflammation |
| title | Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser |
| title_full | Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser |
| title_fullStr | Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser |
| title_full_unstemmed | Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser |
| title_short | Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser |
| title_sort | immunophenotyping of posttraumatic neutrophils on a routine haematology analyser |
| url | http://dx.doi.org/10.1155/2012/509513 |
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