Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells
Vitis vinifera cv. Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system. One-factor-at-a-time experiment revealed that OD600 of Agrobacterium, time of inoculation, co-cultivation, and cell-drying before inoc...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2012-03-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912600239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849247012998873088 |
|---|---|
| author | Jiao WU Ya-li ZHANG Rong-rong HE Lei ZHU Chao-xia WANG Xia XU Jiang LU |
| author_facet | Jiao WU Ya-li ZHANG Rong-rong HE Lei ZHU Chao-xia WANG Xia XU Jiang LU |
| author_sort | Jiao WU |
| collection | DOAJ |
| description | Vitis vinifera cv. Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system. One-factor-at-a-time experiment revealed that OD600 of Agrobacterium, time of inoculation, co-cultivation, and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions: 0.8 of OD600, 25 min of inoculation, 2 d of co-cultivation, and 10 min of cell drying. Response surface methodology experiments based on a five-level, four-factor central-composite rotatable design were then used to optimize these selected factors. The optimized conditions for Chardonnay grape transformation were: 0.8711 of OD600, 28.9 min of inoculation, 2.25 d of co-cultivation and 11.76 min of cell drying. After optimization, transformation efficiency was 26.2% and there were no interactions among different factors. |
| format | Article |
| id | doaj-art-418600b8edb44db08df8159e4a486476 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-418600b8edb44db08df8159e4a4864762025-08-20T03:58:21ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-03-0111338739610.1016/S2095-3119(12)60023-9Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic CellsJiao WU0Ya-li ZHANG1Rong-rong HE2Lei ZHU3Chao-xia WANG4Xia XU5Jiang LU6College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China; WU Jiao, Tel: +86-10-62737465College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China; ZHANG Ya-li, Tel: +86-10-62737465College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. ChinaCenter for Viticulture and Small Fruit Research, Florida A&M University, Tallahassee 32317, USACollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China; Center for Viticulture and Small Fruit Research, Florida A&M University, Tallahassee 32317, USA; Correspondence LU Jiang, Tel: +1-850-412-7393, Fax: +1-850-412-7464Vitis vinifera cv. Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system. One-factor-at-a-time experiment revealed that OD600 of Agrobacterium, time of inoculation, co-cultivation, and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions: 0.8 of OD600, 25 min of inoculation, 2 d of co-cultivation, and 10 min of cell drying. Response surface methodology experiments based on a five-level, four-factor central-composite rotatable design were then used to optimize these selected factors. The optimized conditions for Chardonnay grape transformation were: 0.8711 of OD600, 28.9 min of inoculation, 2.25 d of co-cultivation and 11.76 min of cell drying. After optimization, transformation efficiency was 26.2% and there were no interactions among different factors.http://www.sciencedirect.com/science/article/pii/S2095311912600239transformationChardonnaysuspension culturesresponse surface methodology |
| spellingShingle | Jiao WU Ya-li ZHANG Rong-rong HE Lei ZHU Chao-xia WANG Xia XU Jiang LU Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells Journal of Integrative Agriculture transformation Chardonnay suspension cultures response surface methodology |
| title | Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells |
| title_full | Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells |
| title_fullStr | Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells |
| title_full_unstemmed | Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells |
| title_short | Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera cv. Chardonnay Embryogenic Cells |
| title_sort | optimization of transformation efficiency of suspension cultured vitis vinifera cv chardonnay embryogenic cells |
| topic | transformation Chardonnay suspension cultures response surface methodology |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912600239 |
| work_keys_str_mv | AT jiaowu optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT yalizhang optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT rongronghe optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT leizhu optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT chaoxiawang optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT xiaxu optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells AT jianglu optimizationoftransformationefficiencyofsuspensionculturedvitisviniferacvchardonnayembryogeniccells |