Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation
Microorganisms have the ability to secrete enzymes when they are grown in the presence of certain substrates. Amylases are among the most important industrial enzymes and are of great significance in biotechnological studies. Bacteria belonging to the genus Bacillus were isolated using mannitol egg...
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| Format: | Article |
| Language: | English |
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Syiah Kuala University
2014-09-01
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| Series: | Aceh International Journal of Science and Technology |
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| Online Access: | https://jurnal.usk.ac.id/AIJST/article/view/1592 |
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| author | Helen H. Raplong Peter O. Odeleye Chrinius Hammuel Matthew O. Idoko Judy I. Asanato Evelyn H. Odeke |
| author_facet | Helen H. Raplong Peter O. Odeleye Chrinius Hammuel Matthew O. Idoko Judy I. Asanato Evelyn H. Odeke |
| author_sort | Helen H. Raplong |
| collection | DOAJ |
| description | Microorganisms have the ability to secrete enzymes when they are grown in the presence of certain substrates. Amylases are among the most important industrial enzymes and are of great significance in biotechnological studies. Bacteria belonging to the genus Bacillus were isolated using mannitol egg yolk polymyxin B (MYP) agar a highly selective media for Bacillus cereus isolation. The isolates were tested for α-amylase production on nutrient agar supplemented with starch and in submerged fermentation. The bacteria isolated and identified (using the Microgen Bacillus identification kit) were all Bacillus cereus and SB2 had the largest zone of hydrolysis of 12mm on nutrient agar supplemented with starch as well as the highest enzyme activity of 1.62U/ml. Amylase activity of 2.56U/ml was obtained after 24 hours incubation in submerged fermentation. When amylase enzyme production parameters where optimized, maximum amylase activity was obtained at a pH of 6.5, temperature of 350C, incubation time of 24 hours and 4% inoculums concentration. Bacillus cereus SB2 is a potential isolate for alpha-amylase production with soluble starch as the sole carbon source in submerged fermentation. |
| format | Article |
| id | doaj-art-418306f29a0f49598f6a5d240a451abe |
| institution | Kabale University |
| issn | 2088-9860 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | Aceh International Journal of Science and Technology |
| spelling | doaj-art-418306f29a0f49598f6a5d240a451abe2025-08-20T03:36:19ZengSyiah Kuala UniversityAceh International Journal of Science and Technology2088-98602014-09-013312413010.13170/aijst.3.3.15921516Production of Alpha Amylase by Bacillus cereus in Submerged FermentationHelen H. Raplong0Peter O. Odeleye1Chrinius Hammuel2Matthew O. Idoko3Judy I. Asanato4Evelyn H. Odeke5National Research Institute for Chemical TechnologyAhmadu Bello UniversityNational Research Institute for Chemical TechnologyAhmadu Bello UniversityNational Research Institute for Chemical TechnologyNational Research Institute for Chemical TechnologyMicroorganisms have the ability to secrete enzymes when they are grown in the presence of certain substrates. Amylases are among the most important industrial enzymes and are of great significance in biotechnological studies. Bacteria belonging to the genus Bacillus were isolated using mannitol egg yolk polymyxin B (MYP) agar a highly selective media for Bacillus cereus isolation. The isolates were tested for α-amylase production on nutrient agar supplemented with starch and in submerged fermentation. The bacteria isolated and identified (using the Microgen Bacillus identification kit) were all Bacillus cereus and SB2 had the largest zone of hydrolysis of 12mm on nutrient agar supplemented with starch as well as the highest enzyme activity of 1.62U/ml. Amylase activity of 2.56U/ml was obtained after 24 hours incubation in submerged fermentation. When amylase enzyme production parameters where optimized, maximum amylase activity was obtained at a pH of 6.5, temperature of 350C, incubation time of 24 hours and 4% inoculums concentration. Bacillus cereus SB2 is a potential isolate for alpha-amylase production with soluble starch as the sole carbon source in submerged fermentation.https://jurnal.usk.ac.id/AIJST/article/view/1592bacillus cereusalpha-amylasesubmergedfermentationstarch. |
| spellingShingle | Helen H. Raplong Peter O. Odeleye Chrinius Hammuel Matthew O. Idoko Judy I. Asanato Evelyn H. Odeke Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation Aceh International Journal of Science and Technology bacillus cereus alpha-amylase submerged fermentation starch. |
| title | Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation |
| title_full | Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation |
| title_fullStr | Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation |
| title_full_unstemmed | Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation |
| title_short | Production of Alpha Amylase by Bacillus cereus in Submerged Fermentation |
| title_sort | production of alpha amylase by bacillus cereus in submerged fermentation |
| topic | bacillus cereus alpha-amylase submerged fermentation starch. |
| url | https://jurnal.usk.ac.id/AIJST/article/view/1592 |
| work_keys_str_mv | AT helenhraplong productionofalphaamylasebybacilluscereusinsubmergedfermentation AT peteroodeleye productionofalphaamylasebybacilluscereusinsubmergedfermentation AT chriniushammuel productionofalphaamylasebybacilluscereusinsubmergedfermentation AT matthewoidoko productionofalphaamylasebybacilluscereusinsubmergedfermentation AT judyiasanato productionofalphaamylasebybacilluscereusinsubmergedfermentation AT evelynhodeke productionofalphaamylasebybacilluscereusinsubmergedfermentation |