Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis
Histopathology has been suggested as a reliable method for tumour reduction evaluation of preoperatively treated breast cancer. Immunocytochemistry can be used to enhance the visibility of residual tumour cellularity and in the evaluation of its proliferative activity. We compared Image Analysis (IA...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1998-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/630295 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304642886828032 |
|---|---|
| author | Valentina Corletto Paolo Verderio Roberto Giardini Sonia Cipriani Silvana Di Palma Franco Rilke |
| author_facet | Valentina Corletto Paolo Verderio Roberto Giardini Sonia Cipriani Silvana Di Palma Franco Rilke |
| author_sort | Valentina Corletto |
| collection | DOAJ |
| description | Histopathology has been suggested as a reliable method for tumour reduction evaluation of preoperatively treated breast cancer. Immunocytochemistry can be used to enhance the visibility of residual tumour cellularity and in the evaluation of its proliferative activity. We compared Image Analysis (IA) with Light Microscopy Analysis (LMA) on sections of breast carcinomas treated with preoperative chemo‐ or chemo/radiotherapy in the evaluation of the Neoplastic Cell Density (NCD) (69 cases) and the Proliferation Index (PI) (35 cases). NCD was expressed as the immunoreactive area to cytokeratin over the total original neoplastic area and PI was expressed as the number of immunostained tumoural nuclei with MIB1 MoAb over the total of tumoural nuclei. The intraobserver agreement and that between IA and LMA for both indices were estimated by the common (Kw) and the jackknife weighted kappa statistic (K˜w). The extent of agreement of each considered category was also assessed by means of the category‐specific kappa statistics (Kcs). The intraobserver agreement within LMA for NCD and PI and that between IA and LMA for PI were both satisfactory. Upon evaluation of the NCD, the agreement between IA and LMA showed unsatisfactory results, especially when the ratio between the residual tumour cells and the background was critical. |
| format | Article |
| id | doaj-art-417db41a5d044e6f973357134d3432bb |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-417db41a5d044e6f973357134d3432bb2025-08-20T03:55:40ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-01162839310.1155/1998/630295Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy AnalysisValentina Corletto0Paolo Verderio1Roberto Giardini2Sonia Cipriani3Silvana Di Palma4Franco Rilke5Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyIstituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyIstituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyIstituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyIstituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyIstituto Nazionale per lo Studio e la Cura dei Tumori, Milano, ItalyHistopathology has been suggested as a reliable method for tumour reduction evaluation of preoperatively treated breast cancer. Immunocytochemistry can be used to enhance the visibility of residual tumour cellularity and in the evaluation of its proliferative activity. We compared Image Analysis (IA) with Light Microscopy Analysis (LMA) on sections of breast carcinomas treated with preoperative chemo‐ or chemo/radiotherapy in the evaluation of the Neoplastic Cell Density (NCD) (69 cases) and the Proliferation Index (PI) (35 cases). NCD was expressed as the immunoreactive area to cytokeratin over the total original neoplastic area and PI was expressed as the number of immunostained tumoural nuclei with MIB1 MoAb over the total of tumoural nuclei. The intraobserver agreement and that between IA and LMA for both indices were estimated by the common (Kw) and the jackknife weighted kappa statistic (K˜w). The extent of agreement of each considered category was also assessed by means of the category‐specific kappa statistics (Kcs). The intraobserver agreement within LMA for NCD and PI and that between IA and LMA for PI were both satisfactory. Upon evaluation of the NCD, the agreement between IA and LMA showed unsatisfactory results, especially when the ratio between the residual tumour cells and the background was critical.http://dx.doi.org/10.1155/1998/630295 |
| spellingShingle | Valentina Corletto Paolo Verderio Roberto Giardini Sonia Cipriani Silvana Di Palma Franco Rilke Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis Analytical Cellular Pathology |
| title | Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis |
| title_full | Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis |
| title_fullStr | Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis |
| title_full_unstemmed | Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis |
| title_short | Evaluation of Residual Cellularity and Proliferation on Preoperatively Treated Breast Cancer: A Comparison between Image Analysis and Light Microscopy Analysis |
| title_sort | evaluation of residual cellularity and proliferation on preoperatively treated breast cancer a comparison between image analysis and light microscopy analysis |
| url | http://dx.doi.org/10.1155/1998/630295 |
| work_keys_str_mv | AT valentinacorletto evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis AT paoloverderio evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis AT robertogiardini evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis AT soniacipriani evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis AT silvanadipalma evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis AT francorilke evaluationofresidualcellularityandproliferationonpreoperativelytreatedbreastcanceracomparisonbetweenimageanalysisandlightmicroscopyanalysis |