The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs
To determine the effect of membrane brightness on multifocal electroretinograms (mfERGs), we implanted poly lactic-co-glycolic acid (PLGA) membranes in the subretinal space of 11 porcine eyes. We compared membranes with their native shiny white color with membranes that were stained with a blue dye...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/263264 |
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| author | A. T. Christiansen J. F. Kiilgaard M. Smith R. Ejstrup G. E. Wnek J. U. Prause M. J. Young H. Klassen H. Kaplan M. La Cour |
| author_facet | A. T. Christiansen J. F. Kiilgaard M. Smith R. Ejstrup G. E. Wnek J. U. Prause M. J. Young H. Klassen H. Kaplan M. La Cour |
| author_sort | A. T. Christiansen |
| collection | DOAJ |
| description | To determine the effect of membrane brightness on multifocal electroretinograms (mfERGs), we implanted poly lactic-co-glycolic acid (PLGA) membranes in the subretinal space of 11 porcine eyes. We compared membranes with their native shiny white color with membranes that were stained with a blue dye (Brilliant Blue). Histological and electrophysiological evaluation of the overlying retina was carried out 6 weeks after implantation. Histologically, both white and blue membranes degraded in a spongiform manner leaving a disrupted outer retina with no preserved photoreceptor segments. Multifocal ERG revealed the white membranes to have a significantly higher P1-amplitude ratio than the blue (P=0.027), and a correlation between brightness ratio and P1-amplitude ratio was found (r=0.762). Based on our findings, we conclude that bright subretinal objects can produce normal mfERG amplitude ratios even when the adjacent photoreceptors are missing. Functional assessment with mfERG in scaffold implant studies should therefore be evaluated with care. |
| format | Article |
| id | doaj-art-4179dae5449544c281c696f67d334746 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4179dae5449544c281c696f67d3347462025-02-03T05:57:47ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/263264263264The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in PigsA. T. Christiansen0J. F. Kiilgaard1M. Smith2R. Ejstrup3G. E. Wnek4J. U. Prause5M. J. Young6H. Klassen7H. Kaplan8M. La Cour9Department of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkDepartment of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkNuVention Solutions Inc., Valley View, OH 44125, USADepartment of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkCase Western Reserve University, Cleveland, OH 44106, USAEye Pathology Institute, University of Copenhagen, 2100 Copenhagen, DenmarkSchepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USAThe Gavin Herbert Eye Institute and Stem Cell Research Center, University of California, Irvine, CA 92697, USADepartment of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY 40208, USADepartment of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkTo determine the effect of membrane brightness on multifocal electroretinograms (mfERGs), we implanted poly lactic-co-glycolic acid (PLGA) membranes in the subretinal space of 11 porcine eyes. We compared membranes with their native shiny white color with membranes that were stained with a blue dye (Brilliant Blue). Histological and electrophysiological evaluation of the overlying retina was carried out 6 weeks after implantation. Histologically, both white and blue membranes degraded in a spongiform manner leaving a disrupted outer retina with no preserved photoreceptor segments. Multifocal ERG revealed the white membranes to have a significantly higher P1-amplitude ratio than the blue (P=0.027), and a correlation between brightness ratio and P1-amplitude ratio was found (r=0.762). Based on our findings, we conclude that bright subretinal objects can produce normal mfERG amplitude ratios even when the adjacent photoreceptors are missing. Functional assessment with mfERG in scaffold implant studies should therefore be evaluated with care.http://dx.doi.org/10.1155/2012/263264 |
| spellingShingle | A. T. Christiansen J. F. Kiilgaard M. Smith R. Ejstrup G. E. Wnek J. U. Prause M. J. Young H. Klassen H. Kaplan M. La Cour The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs Stem Cells International |
| title | The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs |
| title_full | The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs |
| title_fullStr | The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs |
| title_full_unstemmed | The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs |
| title_short | The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs |
| title_sort | influence of brightness on functional assessment by mferg a study on scaffolds used in retinal cell transplantation in pigs |
| url | http://dx.doi.org/10.1155/2012/263264 |
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