Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study
This study delves into the therapeutic potential of Qi Ling Gui Fu Prescription (QLGFP) in broiler ascites syndrome (AS) by investigating its impact on the phenotypic transformation of vascular smooth muscle. Utilizing network pharmacology, we identified 267 active ingredients and 120 core targets o...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/12/2/78 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719327336431616 |
|---|---|
| author | Jie Kang Ruiqiang Deng Keyao Wang Huimin Wang Yufeng Han Zhibian Duan |
| author_facet | Jie Kang Ruiqiang Deng Keyao Wang Huimin Wang Yufeng Han Zhibian Duan |
| author_sort | Jie Kang |
| collection | DOAJ |
| description | This study delves into the therapeutic potential of Qi Ling Gui Fu Prescription (QLGFP) in broiler ascites syndrome (AS) by investigating its impact on the phenotypic transformation of vascular smooth muscle. Utilizing network pharmacology, we identified 267 active ingredients and 120 core targets of QLGFP, revealing its multifaceted mechanism of action. Gene enrichment analysis highlighted the pivotal roles of Toll-like receptor, FoxO, and MAPK signaling pathways in QLGFP’s therapeutic effects. Experimental validation in a broiler AS model demonstrated that QLGFP regulated the expression of key markers (SM-22α, OPN, and KLF4) associated with the phenotypic transformation of pulmonary artery vascular smooth muscle (PASMC). Clinical improvements were evident, with a significant reduction in ascites cardiac index (AHI). Furthermore, QLGFP suppressed the protein expression of MAPK1 (ERK1), p-MAPK1, MAPK9 (JNK2), p-MAPK9, MA3.PK14 (P38α), and p-MAPK14, along with downstream factors AP1 and ATF4. These findings suggest that QLGFP effectively prevents and treats AS in broilers by modulating the MAPKs-AP1/ATF4 pathway, thereby inhibiting the phenotypic transformation and proliferation of PASMCs. This study contributes a theoretical foundation for understanding the role of QLGFP in the prevention and treatment of AS in broilers. |
| format | Article |
| id | doaj-art-417543d53df547799929f63a38e9e056 |
| institution | DOAJ |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-417543d53df547799929f63a38e9e0562025-08-20T03:12:10ZengMDPI AGVeterinary Sciences2306-73812025-01-011227810.3390/vetsci12020078Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental StudyJie Kang0Ruiqiang Deng1Keyao Wang2Huimin Wang3Yufeng Han4Zhibian Duan5Science and Technology Center, Fenyang College of Shanxi Medical University, Lüliang 032200, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaThis study delves into the therapeutic potential of Qi Ling Gui Fu Prescription (QLGFP) in broiler ascites syndrome (AS) by investigating its impact on the phenotypic transformation of vascular smooth muscle. Utilizing network pharmacology, we identified 267 active ingredients and 120 core targets of QLGFP, revealing its multifaceted mechanism of action. Gene enrichment analysis highlighted the pivotal roles of Toll-like receptor, FoxO, and MAPK signaling pathways in QLGFP’s therapeutic effects. Experimental validation in a broiler AS model demonstrated that QLGFP regulated the expression of key markers (SM-22α, OPN, and KLF4) associated with the phenotypic transformation of pulmonary artery vascular smooth muscle (PASMC). Clinical improvements were evident, with a significant reduction in ascites cardiac index (AHI). Furthermore, QLGFP suppressed the protein expression of MAPK1 (ERK1), p-MAPK1, MAPK9 (JNK2), p-MAPK9, MA3.PK14 (P38α), and p-MAPK14, along with downstream factors AP1 and ATF4. These findings suggest that QLGFP effectively prevents and treats AS in broilers by modulating the MAPKs-AP1/ATF4 pathway, thereby inhibiting the phenotypic transformation and proliferation of PASMCs. This study contributes a theoretical foundation for understanding the role of QLGFP in the prevention and treatment of AS in broilers.https://www.mdpi.com/2306-7381/12/2/78network pharmacologyQi Ling Gui Fu prescriptionbroiler ascites syndromeMAPK pathwayphenotypic switching of VSMC |
| spellingShingle | Jie Kang Ruiqiang Deng Keyao Wang Huimin Wang Yufeng Han Zhibian Duan Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study Veterinary Sciences network pharmacology Qi Ling Gui Fu prescription broiler ascites syndrome MAPK pathway phenotypic switching of VSMC |
| title | Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study |
| title_full | Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study |
| title_fullStr | Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study |
| title_full_unstemmed | Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study |
| title_short | Mechanistic Insights into the Therapeutic Efficacy of Qi Ling Gui Fu Prescription in Broiler Ascites Syndrome: A Network Pharmacology and Experimental Study |
| title_sort | mechanistic insights into the therapeutic efficacy of qi ling gui fu prescription in broiler ascites syndrome a network pharmacology and experimental study |
| topic | network pharmacology Qi Ling Gui Fu prescription broiler ascites syndrome MAPK pathway phenotypic switching of VSMC |
| url | https://www.mdpi.com/2306-7381/12/2/78 |
| work_keys_str_mv | AT jiekang mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy AT ruiqiangdeng mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy AT keyaowang mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy AT huiminwang mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy AT yufenghan mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy AT zhibianduan mechanisticinsightsintothetherapeuticefficacyofqilingguifuprescriptioninbroilerascitessyndromeanetworkpharmacologyandexperimentalstudy |