SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes
Pericyte loss is an early characteristic change in diabetic retinopathy, but its precise molecular mechanisms have not been elucidated. This study investigated the role of SENP1 in pericyte loss in diabetic retinopathy. We demonstrated that a high concentration of glucose inhibited the expression of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/6392658 |
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| author | Jian Gao Xia Chen Qing Gu Xiaoxiao Liu Xun Xu |
| author_facet | Jian Gao Xia Chen Qing Gu Xiaoxiao Liu Xun Xu |
| author_sort | Jian Gao |
| collection | DOAJ |
| description | Pericyte loss is an early characteristic change in diabetic retinopathy, but its precise molecular mechanisms have not been elucidated. This study investigated the role of SENP1 in pericyte loss in diabetic retinopathy. We demonstrated that a high concentration of glucose inhibited the expression of the Sentrin/SUMO-specific protease 1 (SENP1), which resulted in an increase in DBC1 sumoylation in bovine retinal pericytes (BRPCs). Furthermore, SENP1 overexpression attenuated hyperemia-induced apoptosis of BPRCs, and SENP1 knockdown aggravated this effect. We also provide evidence that DBC1 sumoylation/desumoylation is involved in the SENP1-regulated apoptosis of BRPCs under high glucose conditions. Understanding the role of SENP1 in the pathogenesis of high glucose induced pericyte loss could help elucidate important targets for future pharmacological interventions. |
| format | Article |
| id | doaj-art-41748bafadf24a58be12a63c8a6d4540 |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-41748bafadf24a58be12a63c8a6d45402025-08-20T02:08:25ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/63926586392658SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal PericytesJian Gao0Xia Chen1Qing Gu2Xiaoxiao Liu3Xun Xu4Department of Ophthalmology, Shanghai First People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, ChinaDepartment of Ophthalmology, Shanghai First People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, ChinaDepartment of Ophthalmology, Shanghai First People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, ChinaDepartment of Ophthalmology, Shanghai First People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, ChinaDepartment of Ophthalmology, Shanghai First People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, ChinaPericyte loss is an early characteristic change in diabetic retinopathy, but its precise molecular mechanisms have not been elucidated. This study investigated the role of SENP1 in pericyte loss in diabetic retinopathy. We demonstrated that a high concentration of glucose inhibited the expression of the Sentrin/SUMO-specific protease 1 (SENP1), which resulted in an increase in DBC1 sumoylation in bovine retinal pericytes (BRPCs). Furthermore, SENP1 overexpression attenuated hyperemia-induced apoptosis of BPRCs, and SENP1 knockdown aggravated this effect. We also provide evidence that DBC1 sumoylation/desumoylation is involved in the SENP1-regulated apoptosis of BRPCs under high glucose conditions. Understanding the role of SENP1 in the pathogenesis of high glucose induced pericyte loss could help elucidate important targets for future pharmacological interventions.http://dx.doi.org/10.1155/2016/6392658 |
| spellingShingle | Jian Gao Xia Chen Qing Gu Xiaoxiao Liu Xun Xu SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes Journal of Ophthalmology |
| title | SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes |
| title_full | SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes |
| title_fullStr | SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes |
| title_full_unstemmed | SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes |
| title_short | SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes |
| title_sort | senp1 mediated desumoylation of dbc1 inhibits apoptosis induced by high glucose in bovine retinal pericytes |
| url | http://dx.doi.org/10.1155/2016/6392658 |
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