Genome-Wide Identification of BnaPDAT Family in <i>Brassica napus</i> and the Effect of <i>BnaA02.PDAT1</i> on Seed Oil Content
Studies in multiple species have shown that phospholipid:diacylglycerol acyltransferase (PDAT) and oil bodies are important factors affecting plant oil accumulation. Although the <i>PDAT</i> gene family has been extensively studied in many plants, it has not yet been systematically analy...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/5/1204 |
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| Summary: | Studies in multiple species have shown that phospholipid:diacylglycerol acyltransferase (PDAT) and oil bodies are important factors affecting plant oil accumulation. Although the <i>PDAT</i> gene family has been extensively studied in many plants, it has not yet been systematically analyzed in <i>Brassica napus</i>. In this study, we identified four <i>PDAT</i> family members in <i>B. napus</i>, which were divided into two subfamilies based on phylogenetic analysis. These members share conserved motifs and gene structures, with multiple <i>cis</i>-acting elements related to plant hormones and abiotic stress in their promoter regions. Transcriptome sequencing revealed that most <i>BnaPDAT</i> genes are highly expressed during the late stages of seed development, with expression differences under various abiotic stresses and in materials with varying oleic acid content. To further investigate the effects of the <i>PDAT</i> gene on seed oil content and fatty acid composition in <i>Brassica napus</i>, we constructed transgenic plants overexpressing <i>BnaA02.PDAT1</i> under the control of the 35S promoter. The results showed that compared to wild type (WT), the thousand-seed weight of <i>BnaA02.PDAT1</i> transgenic plants increased significantly by 12.95–14.76%. Additionally, the total oil content in transgenic seeds was 1.86–2.77% higher than that of WT. Furthermore, the fatty acid composition in the seeds was also altered. This study confirms the critical role of <i>BnaPDAT</i> genes in <i>B. napus</i> seed development and their impact on oil accumulation. |
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| ISSN: | 2073-4395 |