Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis
Abstract By affecting the ovarian pool of follicles and their enclosed oocytes, heat stress has an impact on dairy cow fertility. This study aimed to determine how heat shock (HS) during in vitro maturation affected the ability of the bovine cumulus-oocyte complexes (COCs) to develop, as well as the...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-01-01
|
| Series: | Amino Acids |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s00726-023-03370-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850253781901508608 |
|---|---|
| author | Hayder Radhi Hussein Mzedawee Rasoul Kowsar Reza Moradi-Hajidavaloo Roya Shiasi-Sardoabi Khaled Sadeghi Mohammad Hossein Nasr-Esfahani Mehdi Hajian |
| author_facet | Hayder Radhi Hussein Mzedawee Rasoul Kowsar Reza Moradi-Hajidavaloo Roya Shiasi-Sardoabi Khaled Sadeghi Mohammad Hossein Nasr-Esfahani Mehdi Hajian |
| author_sort | Hayder Radhi Hussein Mzedawee |
| collection | DOAJ |
| description | Abstract By affecting the ovarian pool of follicles and their enclosed oocytes, heat stress has an impact on dairy cow fertility. This study aimed to determine how heat shock (HS) during in vitro maturation affected the ability of the bovine cumulus-oocyte complexes (COCs) to develop, as well as their metabolism of amino acids (AAs). In this study, COCs were in vitro matured for 23 h at 38.5 °C (control; n = 322), 39.5 °C (mild HS (MHS); n = 290), or 40.5 °C (severe HS (SHS); n = 245). In comparison to the control group, the MHS and SHS groups significantly decreased the percentage of metaphase-II oocytes, as well as cumulus cell expansion and viability. The SHS decreased the rates of cleavage and blastocyst formation in comparison to the control and MHS. Compared to the control and MHS-COCs, the SHS-COCs produced significantly more phenylalanine, threonine, valine, arginine, alanine, glutamic acid, and citrulline while depleting less leucine, glutamine, and serine. Data showed that SHS-COCs had the highest appearance and turnover of all AAs and essential AAs. Heat shock was positively correlated with the appearance of glutamic acid, glutamine, isoleucine, alanine, serine, valine, phenylalanine, and asparagine. Network analysis identified the relationship between HS and alanine or glutamic acid, as well as the relationship between blastocyst and cleavage rates and ornithine. The findings imply that SHS may have an impact on the quality and metabolism of AAs in COCs. Moreover, the use of a multistep analysis could simply identify the AAs most closely linked to HS and the developmental competence of bovine COCs. |
| format | Article |
| id | doaj-art-416bd15f61b143fca1ab471ee38f1610 |
| institution | OA Journals |
| issn | 1438-2199 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Amino Acids |
| spelling | doaj-art-416bd15f61b143fca1ab471ee38f16102025-08-20T01:57:16ZengSpringerAmino Acids1438-21992024-01-0156111410.1007/s00726-023-03370-6Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysisHayder Radhi Hussein Mzedawee0Rasoul Kowsar1Reza Moradi-Hajidavaloo2Roya Shiasi-Sardoabi3Khaled Sadeghi4Mohammad Hossein Nasr-Esfahani5Mehdi Hajian6Department of Animal Sciences, College of Agriculture, Isfahan University of TechnologyDepartment of Animal Sciences, College of Agriculture, Isfahan University of TechnologyDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECRDepartment of Animal Sciences, College of Agriculture, Isfahan University of TechnologyDepartment of Animal Sciences, College of Agriculture, Isfahan University of TechnologyDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECRDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECRAbstract By affecting the ovarian pool of follicles and their enclosed oocytes, heat stress has an impact on dairy cow fertility. This study aimed to determine how heat shock (HS) during in vitro maturation affected the ability of the bovine cumulus-oocyte complexes (COCs) to develop, as well as their metabolism of amino acids (AAs). In this study, COCs were in vitro matured for 23 h at 38.5 °C (control; n = 322), 39.5 °C (mild HS (MHS); n = 290), or 40.5 °C (severe HS (SHS); n = 245). In comparison to the control group, the MHS and SHS groups significantly decreased the percentage of metaphase-II oocytes, as well as cumulus cell expansion and viability. The SHS decreased the rates of cleavage and blastocyst formation in comparison to the control and MHS. Compared to the control and MHS-COCs, the SHS-COCs produced significantly more phenylalanine, threonine, valine, arginine, alanine, glutamic acid, and citrulline while depleting less leucine, glutamine, and serine. Data showed that SHS-COCs had the highest appearance and turnover of all AAs and essential AAs. Heat shock was positively correlated with the appearance of glutamic acid, glutamine, isoleucine, alanine, serine, valine, phenylalanine, and asparagine. Network analysis identified the relationship between HS and alanine or glutamic acid, as well as the relationship between blastocyst and cleavage rates and ornithine. The findings imply that SHS may have an impact on the quality and metabolism of AAs in COCs. Moreover, the use of a multistep analysis could simply identify the AAs most closely linked to HS and the developmental competence of bovine COCs.https://doi.org/10.1007/s00726-023-03370-6Heat shockBovine cumulus-oocyte complexesAmino acidsIn vitro maturationMultistep analysis |
| spellingShingle | Hayder Radhi Hussein Mzedawee Rasoul Kowsar Reza Moradi-Hajidavaloo Roya Shiasi-Sardoabi Khaled Sadeghi Mohammad Hossein Nasr-Esfahani Mehdi Hajian Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis Amino Acids Heat shock Bovine cumulus-oocyte complexes Amino acids In vitro maturation Multistep analysis |
| title | Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis |
| title_full | Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis |
| title_fullStr | Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis |
| title_full_unstemmed | Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis |
| title_short | Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis |
| title_sort | heat shock interferes with the amino acid metabolism of bovine cumulus oocyte complexes in vitro a multistep analysis |
| topic | Heat shock Bovine cumulus-oocyte complexes Amino acids In vitro maturation Multistep analysis |
| url | https://doi.org/10.1007/s00726-023-03370-6 |
| work_keys_str_mv | AT hayderradhihusseinmzedawee heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT rasoulkowsar heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT rezamoradihajidavaloo heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT royashiasisardoabi heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT khaledsadeghi heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT mohammadhosseinnasresfahani heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis AT mehdihajian heatshockinterfereswiththeaminoacidmetabolismofbovinecumulusoocytecomplexesinvitroamultistepanalysis |