Isolation, characterization, antidiabetic and antityrosinase potentials of compounds isolated from Anabasis Articulata
Herein, we report the isolation, characterization, in silico evaluation and biological potentials of four compounds (1–4) isolated from A. Articulata. All compounds (isoleteolin (2), hyperoside (3) and quercitrin (4)) demonstrated antioxidant properties; notably, quercetin (1) exhibited exceptional...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Applied Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225002574 |
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| Summary: | Herein, we report the isolation, characterization, in silico evaluation and biological potentials of four compounds (1–4) isolated from A. Articulata. All compounds (isoleteolin (2), hyperoside (3) and quercitrin (4)) demonstrated antioxidant properties; notably, quercetin (1) exhibited exceptional free-radical scavenging activity (87.75 % DPPH and 85.75 % ABTS at 1000 µg/mL) with an IC50 value of 62 µg/mL, and 35 µg/mL, respectively against the tested radicals. The antidiabetic potential was revealed through the inhibition of α-glucosidase and α-amylase. All 4 compounds exhibited α-glucosidase activity (IC50 =32–110 µg/mL) and α-amylase (30–59 µg/mL), among them quercetin showed maximum inhibition of 89.92 % at 1000 µg/mL against α-glucosidase with an IC50 value of 32 µg/mL, and 85.98 % inhibition at 1000 µg/mL against α-amylase with an IC50 value of 30 µg/mL. Next, along with quercetin, all compounds displayed dose-dependent behaviour in tyrosinase inhibition with IC50 values ranging from 4.14–75.99 µM, whereas Lineweaver Burk and Dixon plots, displayed that quercetin with potent inhibition exhibited a competitive mode of inhibition. Further, in silico analyses validated the enzymatic binding affinity of quercetin with docking scores against α-glucosidase (-5.37 kcal/mol), α-amylase (-5.82 kcal/mol), and tyrosinase (-5.37 kcal/mol), respectively. ADMET study revealed that quercetin possesses adequate water solubility, high bioavailability, and low toxicity. These results imply that quercetin has a great deal of promise as a treatment for diabetes, hyperpigmentation disorders, and oxidative stress. |
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| ISSN: | 2772-5022 |