Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss
Experiments on non-inbred anesthetized albino male rats weighing 250-350 g have established that blood reperfusion after acute massive blood loss substantially increases the count of micronucleated (MN) erythrocytes in the rat bone marrow by 8.4 times as a whole as compared with the control, which s...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2005-06-01
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| Series: | Общая реаниматология |
| Online Access: | https://www.reanimatology.com/rmt/article/view/1265 |
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| author | I. L. Kozhura N. V. Kondakova T. A. Tlatova A. K. Kirsanova |
| author_facet | I. L. Kozhura N. V. Kondakova T. A. Tlatova A. K. Kirsanova |
| author_sort | I. L. Kozhura |
| collection | DOAJ |
| description | Experiments on non-inbred anesthetized albino male rats weighing 250-350 g have established that blood reperfusion after acute massive blood loss substantially increases the count of micronucleated (MN) erythrocytes in the rat bone marrow by 8.4 times as a whole as compared with the control, which suggests genomic destabilization. The optimum time of elaboration of MN polychromatic erythrocytes is 24 hours after acute blood loss or reinfusion. The intraarterial administration of mexidole (50 mg/kg) just before blood reinfusion drastically decreases the count of MN erythrocytes virtually to the that seen in control animals. The observed effects during micronucleus assay generally correspond to the changes in lipid per-oxidation and overall antioxidative activity, pointing to the free radical mechanism of this phenomenon. |
| format | Article |
| id | doaj-art-4164abeea1c04d47a9e40f2c61dd1b80 |
| institution | Kabale University |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2005-06-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-4164abeea1c04d47a9e40f2c61dd1b802025-08-20T03:59:40ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102005-06-0113212410.15360/1813-9779-2005-3-21-241265Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood LossI. L. Kozhura0N. V. Kondakova1T. A. Tlatova2A. K. Kirsanova3Research Institute of General Reanimatology, Russian Academy of Medical Sciences; Research and Training Center of Biomedical Technologies, All-Russian Institute of Medicinal and Aromatic Plants, Russian Academy of Agricultural Sciences, MoscowRussian Academy of Medical Sciences; Research and Training Center of Biomedical Technologies, All-Russian Institute of Medicinal and Aromatic Plants, Russian Academy of Agricultural Sciences, MoscowRussian Academy of Medical Sciences; Research and Training Center of Biomedical Technologies, All-Russian Institute of Medicinal and Aromatic Plants, Russian Academy of Agricultural Sciences, MoscowRussian Academy of Medical Sciences; Research and Training Center of Biomedical Technologies, All-Russian Institute of Medicinal and Aromatic Plants, Russian Academy of Agricultural Sciences, MoscowExperiments on non-inbred anesthetized albino male rats weighing 250-350 g have established that blood reperfusion after acute massive blood loss substantially increases the count of micronucleated (MN) erythrocytes in the rat bone marrow by 8.4 times as a whole as compared with the control, which suggests genomic destabilization. The optimum time of elaboration of MN polychromatic erythrocytes is 24 hours after acute blood loss or reinfusion. The intraarterial administration of mexidole (50 mg/kg) just before blood reinfusion drastically decreases the count of MN erythrocytes virtually to the that seen in control animals. The observed effects during micronucleus assay generally correspond to the changes in lipid per-oxidation and overall antioxidative activity, pointing to the free radical mechanism of this phenomenon.https://www.reanimatology.com/rmt/article/view/1265 |
| spellingShingle | I. L. Kozhura N. V. Kondakova T. A. Tlatova A. K. Kirsanova Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss Общая реаниматология |
| title | Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss |
| title_full | Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss |
| title_fullStr | Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss |
| title_full_unstemmed | Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss |
| title_short | Impact of Reperfusion on the Micronucleus Generation Phenomenon in the Bone Marrow Red Blood Cells in Massive Blood Loss |
| title_sort | impact of reperfusion on the micronucleus generation phenomenon in the bone marrow red blood cells in massive blood loss |
| url | https://www.reanimatology.com/rmt/article/view/1265 |
| work_keys_str_mv | AT ilkozhura impactofreperfusiononthemicronucleusgenerationphenomenoninthebonemarrowredbloodcellsinmassivebloodloss AT nvkondakova impactofreperfusiononthemicronucleusgenerationphenomenoninthebonemarrowredbloodcellsinmassivebloodloss AT tatlatova impactofreperfusiononthemicronucleusgenerationphenomenoninthebonemarrowredbloodcellsinmassivebloodloss AT akkirsanova impactofreperfusiononthemicronucleusgenerationphenomenoninthebonemarrowredbloodcellsinmassivebloodloss |