EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH

Aim. The study of mononucleotide polymorphisms associations rs20455 gene KIF6, rs7439293 gene PALLD, rs2298566 gene SNX19, rs3900940 gene MYH15, rs1010 gene VAMP8, included into the Riskometer of ischemic heart disease of the Celera Corporation (USA), in sudden cardiac death.Material and methods. To...

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Main Authors: V. N. Мaksimov, A. A. Ivanova, P. S. Orlov, S. V. Savchenko, M. I. Voevoda
Format: Article
Language:Russian
Published: «FIRMA «SILICEA» LLC 2015-10-01
Series:Российский кардиологический журнал
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Online Access:https://russjcardiol.elpub.ru/jour/article/view/257
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author V. N. Мaksimov
A. A. Ivanova
P. S. Orlov
S. V. Savchenko
M. I. Voevoda
author_facet V. N. Мaksimov
A. A. Ivanova
P. S. Orlov
S. V. Savchenko
M. I. Voevoda
author_sort V. N. Мaksimov
collection DOAJ
description Aim. The study of mononucleotide polymorphisms associations rs20455 gene KIF6, rs7439293 gene PALLD, rs2298566 gene SNX19, rs3900940 gene MYH15, rs1010 gene VAMP8, included into the Riskometer of ischemic heart disease of the Celera Corporation (USA), in sudden cardiac death.Material and methods. To the study the group of persons included, died from sudden cardiac death, according to the WHO criteria (n=352, mean age — 53,3±8,9 y.), control group from matched by age and gender from DNA bank of HAPIEE, MONICA studies (n=381, mean age — 53,1±8,3 y.), adolescents group (n=296, mean age — 15,6±0,9 y.). DNA extracted via the phenol-chloroform extraction from myocardium of the dyed due to sudden cardiac death, and from venous blood of controls. Genotyping performed with Real-time PCR method using the Taq-Mancatheters technology (Applied Biosystems).Results. The prevalence of alleles rs7439293 gene PALLD, rs2298566 gene SNX19, rs3900940 gene MYH15 did not statistically significantly differ among the groups. In those died suddenly older than 50 years, there was significant decrease of carriers of GG polymorphism rs20455 gene KIF6 (9,0%) comparing to the controls (17,8%) (р=0,009, OR=0,456, 95% CI 0,256-0,810). In men older 50, died suddenly, there was significant decrease of the carriers of genotype СС (8,0%) (р=0,002, OR=0,328, 95% CI 0,159-0,678) and increase of СТ genotype (49,6%) (р=0,025, OR=1,729, 95% CI 1,084-2,758) polymorphism rs1010 gene VAMP8 comparing to the controls (21,0%, 36,3%, respectively). Conclusion. Polymorphisms rs20455 gene KIF6 and rs1010 gene VAMP8 are associated with sudden cardiac death.
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spelling doaj-art-415a7b27a94a4d2caab1767ca820e8692025-08-20T02:59:37Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202015-10-01010121810.15829/1560-4071-2015-10-12-18257EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATHV. N. Мaksimov0A. A. Ivanova1P. S. Orlov2S. V. Savchenko3M. I. Voevoda4Scientific-Research Institute of Therapy and Prevention Medicine of SD RAS, Novosibirsk; Novosibirsk State Medical UniversityScientific-Research Institute of Therapy and Prevention Medicine of SD RAS, NovosibirskScientific-Research Institute of Therapy and Prevention Medicine of SD RAS, Novosibirsk; Institute of Cytology and Genetics of Siberian RAS Department, NovosibirskNovosibirsk State Medical University; Novosibirsk Regional Bureau of Forensic Medicine, NovosibirskScientific-Research Institute of Therapy and Prevention Medicine of SD RAS, Novosibirsk; Institute of Cytology and Genetics of Siberian RAS Department, NovosibirskAim. The study of mononucleotide polymorphisms associations rs20455 gene KIF6, rs7439293 gene PALLD, rs2298566 gene SNX19, rs3900940 gene MYH15, rs1010 gene VAMP8, included into the Riskometer of ischemic heart disease of the Celera Corporation (USA), in sudden cardiac death.Material and methods. To the study the group of persons included, died from sudden cardiac death, according to the WHO criteria (n=352, mean age — 53,3±8,9 y.), control group from matched by age and gender from DNA bank of HAPIEE, MONICA studies (n=381, mean age — 53,1±8,3 y.), adolescents group (n=296, mean age — 15,6±0,9 y.). DNA extracted via the phenol-chloroform extraction from myocardium of the dyed due to sudden cardiac death, and from venous blood of controls. Genotyping performed with Real-time PCR method using the Taq-Mancatheters technology (Applied Biosystems).Results. The prevalence of alleles rs7439293 gene PALLD, rs2298566 gene SNX19, rs3900940 gene MYH15 did not statistically significantly differ among the groups. In those died suddenly older than 50 years, there was significant decrease of carriers of GG polymorphism rs20455 gene KIF6 (9,0%) comparing to the controls (17,8%) (р=0,009, OR=0,456, 95% CI 0,256-0,810). In men older 50, died suddenly, there was significant decrease of the carriers of genotype СС (8,0%) (р=0,002, OR=0,328, 95% CI 0,159-0,678) and increase of СТ genotype (49,6%) (р=0,025, OR=1,729, 95% CI 1,084-2,758) polymorphism rs1010 gene VAMP8 comparing to the controls (21,0%, 36,3%, respectively). Conclusion. Polymorphisms rs20455 gene KIF6 and rs1010 gene VAMP8 are associated with sudden cardiac death.https://russjcardiol.elpub.ru/jour/article/view/257sudden cardiac deathmononucleotide polymorphismriskometergene rs20455kif6rs7439293palldrs2298566snx19rs3900940myh15rs1010vamp8
spellingShingle V. N. Мaksimov
A. A. Ivanova
P. S. Orlov
S. V. Savchenko
M. I. Voevoda
EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
Российский кардиологический журнал
sudden cardiac death
mononucleotide polymorphism
riskometer
gene rs20455
kif6
rs7439293
palld
rs2298566
snx19
rs3900940
myh15
rs1010
vamp8
title EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
title_full EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
title_fullStr EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
title_full_unstemmed EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
title_short EVALUATION OF THE GENETIC RISKOMETER COMPONENTS KIF6, PALLD, SNX19, MYH15, VAMP8 FOR ISCHEMIC HEART DISEASE WITH SUDDEN CARDIAC DEATH
title_sort evaluation of the genetic riskometer components kif6 palld snx19 myh15 vamp8 for ischemic heart disease with sudden cardiac death
topic sudden cardiac death
mononucleotide polymorphism
riskometer
gene rs20455
kif6
rs7439293
palld
rs2298566
snx19
rs3900940
myh15
rs1010
vamp8
url https://russjcardiol.elpub.ru/jour/article/view/257
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AT aaivanova evaluationofthegeneticriskometercomponentskif6palldsnx19myh15vamp8forischemicheartdiseasewithsuddencardiacdeath
AT psorlov evaluationofthegeneticriskometercomponentskif6palldsnx19myh15vamp8forischemicheartdiseasewithsuddencardiacdeath
AT svsavchenko evaluationofthegeneticriskometercomponentskif6palldsnx19myh15vamp8forischemicheartdiseasewithsuddencardiacdeath
AT mivoevoda evaluationofthegeneticriskometercomponentskif6palldsnx19myh15vamp8forischemicheartdiseasewithsuddencardiacdeath