Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer)
The green peach aphid, <i>Myzus persicae</i>, is a notorious pest worldwide. We collected a field population of the pest (FZQ-F) that exhibited high resistance to neonicotinoids. Exposure to neonicotinoids can induce oxidative damage in animals; however, it remains unclear whether antiox...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/13/12/1453 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850059205692620800 |
|---|---|
| author | Wenhua Rao Feng Chen Xianzhi Zhou Jun Wang Lei Lin Guocheng Fan Jinfeng Hu |
| author_facet | Wenhua Rao Feng Chen Xianzhi Zhou Jun Wang Lei Lin Guocheng Fan Jinfeng Hu |
| author_sort | Wenhua Rao |
| collection | DOAJ |
| description | The green peach aphid, <i>Myzus persicae</i>, is a notorious pest worldwide. We collected a field population of the pest (FZQ-F) that exhibited high resistance to neonicotinoids. Exposure to neonicotinoids can induce oxidative damage in animals; however, it remains unclear whether antioxidant enzymes contribute to the innate immune response of neonicotinoid-resistant pests against high doses of insecticides. Treatment with sublethal doses of thiacloprid (LC<sub>10</sub> and LC<sub>25</sub>) for 3, 6, 12, 24, 48, and 72 h resulted in significantly increased reactive oxygen species (ROS), including H<sub>2</sub>O<sub>2</sub> content, in FZQ-F adults, indicating insecticide-induced oxidative stress. Additionally, the peroxidase activity in FZQ-F adults increased after thiacloprid exposure. Using comparative genomics, we identified 31 heme peroxidases in <i>M. persicae</i> with a typical “2Cys” structure, and phylogenetic analyses divided them into five groups. Comparative transcriptomes revealed that MpPxd2 and MpPxd4 were significantly upregulated in thiacloprid-treated aphids. Thiacloprid exposure significantly induced MpPxd2 and MpPxd4 expression levels, consistent with high H<sub>2</sub>O<sub>2</sub> content and peroxidase activity. The knockdown of MpPxd2 or MpPxd4 in FZQ-F increased their susceptibility to imidacloprid, thiacloprid, and thiamethoxam, verifying the protective role of the heme peroxidases against neonicotinoids in aphids. The knockdown of MpPxd2 or MpPxd4 also led to shorter longevity and a low fecundity of adult aphids at 31 °C compared to controls. The results show that MpPxd2 or MpPxd4 is important in how cells respond to oxidative stress and may help resistant <i>M. persicae</i> pests to handle neonicotinoids. |
| format | Article |
| id | doaj-art-41579d6fd5484fe3b5f7900a31e9c46e |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-41579d6fd5484fe3b5f7900a31e9c46e2025-08-20T02:50:56ZengMDPI AGAntioxidants2076-39212024-11-011312145310.3390/antiox13121453Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer)Wenhua Rao0Feng Chen1Xianzhi Zhou2Jun Wang3Lei Lin4Guocheng Fan5Jinfeng Hu6Fujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaThe green peach aphid, <i>Myzus persicae</i>, is a notorious pest worldwide. We collected a field population of the pest (FZQ-F) that exhibited high resistance to neonicotinoids. Exposure to neonicotinoids can induce oxidative damage in animals; however, it remains unclear whether antioxidant enzymes contribute to the innate immune response of neonicotinoid-resistant pests against high doses of insecticides. Treatment with sublethal doses of thiacloprid (LC<sub>10</sub> and LC<sub>25</sub>) for 3, 6, 12, 24, 48, and 72 h resulted in significantly increased reactive oxygen species (ROS), including H<sub>2</sub>O<sub>2</sub> content, in FZQ-F adults, indicating insecticide-induced oxidative stress. Additionally, the peroxidase activity in FZQ-F adults increased after thiacloprid exposure. Using comparative genomics, we identified 31 heme peroxidases in <i>M. persicae</i> with a typical “2Cys” structure, and phylogenetic analyses divided them into five groups. Comparative transcriptomes revealed that MpPxd2 and MpPxd4 were significantly upregulated in thiacloprid-treated aphids. Thiacloprid exposure significantly induced MpPxd2 and MpPxd4 expression levels, consistent with high H<sub>2</sub>O<sub>2</sub> content and peroxidase activity. The knockdown of MpPxd2 or MpPxd4 in FZQ-F increased their susceptibility to imidacloprid, thiacloprid, and thiamethoxam, verifying the protective role of the heme peroxidases against neonicotinoids in aphids. The knockdown of MpPxd2 or MpPxd4 also led to shorter longevity and a low fecundity of adult aphids at 31 °C compared to controls. The results show that MpPxd2 or MpPxd4 is important in how cells respond to oxidative stress and may help resistant <i>M. persicae</i> pests to handle neonicotinoids.https://www.mdpi.com/2076-3921/13/12/1453<i>Myzus persicae</i>antioxidant defenseheme peroxidasethiacloprid exposureneonicotinoid resistance |
| spellingShingle | Wenhua Rao Feng Chen Xianzhi Zhou Jun Wang Lei Lin Guocheng Fan Jinfeng Hu Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) Antioxidants <i>Myzus persicae</i> antioxidant defense heme peroxidase thiacloprid exposure neonicotinoid resistance |
| title | Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) |
| title_full | Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) |
| title_fullStr | Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) |
| title_full_unstemmed | Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) |
| title_short | Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant <i>Myzus persicae</i> (Sulzer) |
| title_sort | function analysis of heme peroxidase genes mppxd2 and mppxd4 under thiacloprid exposure in the neonicotinoid resistant i myzus persicae i sulzer |
| topic | <i>Myzus persicae</i> antioxidant defense heme peroxidase thiacloprid exposure neonicotinoid resistance |
| url | https://www.mdpi.com/2076-3921/13/12/1453 |
| work_keys_str_mv | AT wenhuarao functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT fengchen functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT xianzhizhou functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT junwang functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT leilin functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT guochengfan functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer AT jinfenghu functionanalysisofhemeperoxidasegenesmppxd2andmppxd4underthiaclopridexposureintheneonicotinoidresistantimyzuspersicaeisulzer |